GANGLIOSIDES OF HUMAN MYELIN: SIALOSYLGALACTOSYLCERAMIDE (G7) AS A MAJOR COMPONENT

R. W. Ledeen, Robert K Yu, L. F. Eng

Research output: Contribution to journalArticle

463 Citations (Scopus)

Abstract

Abstract— Gangliosides were isolated from purified human myelin in a yield of 62 μg of lipid‐bound sialic acid per 100 mg of dry myelin. Sialosylgalactosyl ceramide (G7) was found to be a major component of the ganglioside fraction, amounting to 15 per cent of the total sialic acid. It accounted for 10 per cent of lipid‐bound sialic acid in adult human white matter, making it the third most abundant ganglioside on a molar basis. These results were obtained with an improved method for isolating total gangliosides in high yield, by employing DEAE‐Sephadex column chromatography. Myelin from other mammalian species had considerably less G7, and there were also indications of maturational changes. Both 2‐hydroxy and unsubstituted fatty acids were components of the ceramide unit, in a ratio of 3:2, respectively. The overall fatty acid pattern was very similar to that for myelin cerebroside and sulphatide. Long‐chain bases included only C18 species, with sphingosine predominating (>90 per cent). These observations suggest a metabolic relationship between G7 and either cerebroside or sulphatide.

Original languageEnglish (US)
Pages (from-to)829-839
Number of pages11
JournalJournal of Neurochemistry
Volume21
Issue number4
DOIs
StatePublished - Jan 1 1973
Externally publishedYes

Fingerprint

Gangliosides
Myelin Sheath
N-Acetylneuraminic Acid
Cerebrosides
Sulfoglycosphingolipids
Ceramides
Fatty Acids
Column chromatography
Sphingosine
Chromatography

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

GANGLIOSIDES OF HUMAN MYELIN : SIALOSYLGALACTOSYLCERAMIDE (G7) AS A MAJOR COMPONENT. / Ledeen, R. W.; Yu, Robert K; Eng, L. F.

In: Journal of Neurochemistry, Vol. 21, No. 4, 01.01.1973, p. 829-839.

Research output: Contribution to journalArticle

@article{c276229d2d004e05a3f60dd0c7ed40c8,
title = "GANGLIOSIDES OF HUMAN MYELIN: SIALOSYLGALACTOSYLCERAMIDE (G7) AS A MAJOR COMPONENT",
abstract = "Abstract— Gangliosides were isolated from purified human myelin in a yield of 62 μg of lipid‐bound sialic acid per 100 mg of dry myelin. Sialosylgalactosyl ceramide (G7) was found to be a major component of the ganglioside fraction, amounting to 15 per cent of the total sialic acid. It accounted for 10 per cent of lipid‐bound sialic acid in adult human white matter, making it the third most abundant ganglioside on a molar basis. These results were obtained with an improved method for isolating total gangliosides in high yield, by employing DEAE‐Sephadex column chromatography. Myelin from other mammalian species had considerably less G7, and there were also indications of maturational changes. Both 2‐hydroxy and unsubstituted fatty acids were components of the ceramide unit, in a ratio of 3:2, respectively. The overall fatty acid pattern was very similar to that for myelin cerebroside and sulphatide. Long‐chain bases included only C18 species, with sphingosine predominating (>90 per cent). These observations suggest a metabolic relationship between G7 and either cerebroside or sulphatide.",
author = "Ledeen, {R. W.} and Yu, {Robert K} and Eng, {L. F.}",
year = "1973",
month = "1",
day = "1",
doi = "10.1111/j.1471-4159.1973.tb07527.x",
language = "English (US)",
volume = "21",
pages = "829--839",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - GANGLIOSIDES OF HUMAN MYELIN

T2 - SIALOSYLGALACTOSYLCERAMIDE (G7) AS A MAJOR COMPONENT

AU - Ledeen, R. W.

AU - Yu, Robert K

AU - Eng, L. F.

PY - 1973/1/1

Y1 - 1973/1/1

N2 - Abstract— Gangliosides were isolated from purified human myelin in a yield of 62 μg of lipid‐bound sialic acid per 100 mg of dry myelin. Sialosylgalactosyl ceramide (G7) was found to be a major component of the ganglioside fraction, amounting to 15 per cent of the total sialic acid. It accounted for 10 per cent of lipid‐bound sialic acid in adult human white matter, making it the third most abundant ganglioside on a molar basis. These results were obtained with an improved method for isolating total gangliosides in high yield, by employing DEAE‐Sephadex column chromatography. Myelin from other mammalian species had considerably less G7, and there were also indications of maturational changes. Both 2‐hydroxy and unsubstituted fatty acids were components of the ceramide unit, in a ratio of 3:2, respectively. The overall fatty acid pattern was very similar to that for myelin cerebroside and sulphatide. Long‐chain bases included only C18 species, with sphingosine predominating (>90 per cent). These observations suggest a metabolic relationship between G7 and either cerebroside or sulphatide.

AB - Abstract— Gangliosides were isolated from purified human myelin in a yield of 62 μg of lipid‐bound sialic acid per 100 mg of dry myelin. Sialosylgalactosyl ceramide (G7) was found to be a major component of the ganglioside fraction, amounting to 15 per cent of the total sialic acid. It accounted for 10 per cent of lipid‐bound sialic acid in adult human white matter, making it the third most abundant ganglioside on a molar basis. These results were obtained with an improved method for isolating total gangliosides in high yield, by employing DEAE‐Sephadex column chromatography. Myelin from other mammalian species had considerably less G7, and there were also indications of maturational changes. Both 2‐hydroxy and unsubstituted fatty acids were components of the ceramide unit, in a ratio of 3:2, respectively. The overall fatty acid pattern was very similar to that for myelin cerebroside and sulphatide. Long‐chain bases included only C18 species, with sphingosine predominating (>90 per cent). These observations suggest a metabolic relationship between G7 and either cerebroside or sulphatide.

UR - http://www.scopus.com/inward/record.url?scp=0015822234&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015822234&partnerID=8YFLogxK

U2 - 10.1111/j.1471-4159.1973.tb07527.x

DO - 10.1111/j.1471-4159.1973.tb07527.x

M3 - Article

C2 - 4754859

AN - SCOPUS:0015822234

VL - 21

SP - 829

EP - 839

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 4

ER -