Gangliosides of normal and neoplastic human melanocytes

John M. Carubia, Robert K. Yu, Larry J. Macala, John M. Kirkwood, Janos M. Varga

Research output: Contribution to journalArticlepeer-review

120 Scopus citations

Abstract

The major ganglioside component isolated from diploid human melanocytes is sialosyllactosylceramide (GM3 86-91% of total sialic acid). The corresponding disialo derivative (GD3) is found as a minor component (2-6% of total sialic acid) in the membranes of these cells. In human melanoma cells, grown in tissue culture, GD3 is the predominant ganglioside component (48-63% of total sialic acid). Withdrawal of TPA from the culture medium of normal melanocytes or addition of TPA to the medium of melanoma cells had no significant effect on GM3 GD3 ratios. We conclude that the difference between the composition of gangliosides is related to the normal vs transformed phenotypes of melanocytes.

Original languageEnglish (US)
Pages (from-to)500-504
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume120
Issue number2
DOIs
StatePublished - Apr 30 1984
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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