Garlic extract attenuates immobilization stress-induced alterations in plasma antioxidant/oxidant parameters and hepatic function in rats

Syed Kashif Zaidi, Shakeel Ahmed Ansari, Shams Tabrez, MD Nasrul Hoda, Ghulam Md Ashraf, Mohd Shahnawaz Khan, Sultan Alnohair, Naheed Banu, Mohammed Hussain Al-Qahtani

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objective: To investigate the oxidative stress induced by 6 h of immobilization stress in Albino Wistar rats. Further, the pre- and post-treatment of aqueous garlic extract was studied to evaluate its preventive and curative efficacy on stress-induced altered oxidative parameters in rats. Methods: Albino Wistar rats were exposed to 6 h of immobilization stress, and received garlic extract (100 mg/kg body weight) treatment pre- or post-stress exposure. The oxidative status of plasma after various treatments were evaluated by determining the levels of reduced glutathione, glucose, uric acid, thiobarbituric acid reactive substances, aspartate aminotransferase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and the activities of superoxide dismutase, catalase and glutathione-S-transferase by standardized procedures. Results: Immobilization of rats generated oxidative stress in rat plasma, by decreasing the activities of antioxidant enzymes, glutathione levels and glucose, while increasing the lipid peroxidation, serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, ALP and uric acid compared to the non-stressed controls (P<0.01). The garlic extract administration both pre- and post-stress exposure significantly prevented the rise in the diagnostic liver enzymes and reverted the decrease of antioxidant enzymatic activities compared to the stressed group (P<0.05 or P <0.01). Post-stress treatment of extract was found more effective than pre-stress treatment in reverting the values back to normal (P<0.01). Conclusion: Garlic extract seems promising as a nutritional supplement for scavenging free radicals generated in the plasma and to prevent resulting oxidative stress.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalChinese Journal of Integrative Medicine
DOIs
StateAccepted/In press - Feb 15 2017

Fingerprint

Garlic
Oxidants
Immobilization
Antioxidants
Oxidative Stress
Liver
Aspartate Aminotransferases
Uric Acid
Alanine Transaminase
Glutathione
Alkaline Phosphatase
Wistar Rats
Glucose
Thiobarbituric Acid Reactive Substances
Enzymes
Glutathione Transferase
Catalase
Lipid Peroxidation
Superoxide Dismutase
Free Radicals

Keywords

  • alanine transaminase
  • antioxidant enzymes
  • aspartate aminotransferase
  • garlic
  • malondialdehyde
  • stress

ASJC Scopus subject areas

  • Complementary and alternative medicine
  • Pharmacology (medical)

Cite this

Garlic extract attenuates immobilization stress-induced alterations in plasma antioxidant/oxidant parameters and hepatic function in rats. / Zaidi, Syed Kashif; Ansari, Shakeel Ahmed; Tabrez, Shams; Hoda, MD Nasrul; Ashraf, Ghulam Md; Khan, Mohd Shahnawaz; Alnohair, Sultan; Banu, Naheed; Al-Qahtani, Mohammed Hussain.

In: Chinese Journal of Integrative Medicine, 15.02.2017, p. 1-7.

Research output: Contribution to journalArticle

Zaidi, Syed Kashif ; Ansari, Shakeel Ahmed ; Tabrez, Shams ; Hoda, MD Nasrul ; Ashraf, Ghulam Md ; Khan, Mohd Shahnawaz ; Alnohair, Sultan ; Banu, Naheed ; Al-Qahtani, Mohammed Hussain. / Garlic extract attenuates immobilization stress-induced alterations in plasma antioxidant/oxidant parameters and hepatic function in rats. In: Chinese Journal of Integrative Medicine. 2017 ; pp. 1-7.
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abstract = "Objective: To investigate the oxidative stress induced by 6 h of immobilization stress in Albino Wistar rats. Further, the pre- and post-treatment of aqueous garlic extract was studied to evaluate its preventive and curative efficacy on stress-induced altered oxidative parameters in rats. Methods: Albino Wistar rats were exposed to 6 h of immobilization stress, and received garlic extract (100 mg/kg body weight) treatment pre- or post-stress exposure. The oxidative status of plasma after various treatments were evaluated by determining the levels of reduced glutathione, glucose, uric acid, thiobarbituric acid reactive substances, aspartate aminotransferase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and the activities of superoxide dismutase, catalase and glutathione-S-transferase by standardized procedures. Results: Immobilization of rats generated oxidative stress in rat plasma, by decreasing the activities of antioxidant enzymes, glutathione levels and glucose, while increasing the lipid peroxidation, serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, ALP and uric acid compared to the non-stressed controls (P<0.01). The garlic extract administration both pre- and post-stress exposure significantly prevented the rise in the diagnostic liver enzymes and reverted the decrease of antioxidant enzymatic activities compared to the stressed group (P<0.05 or P <0.01). Post-stress treatment of extract was found more effective than pre-stress treatment in reverting the values back to normal (P<0.01). Conclusion: Garlic extract seems promising as a nutritional supplement for scavenging free radicals generated in the plasma and to prevent resulting oxidative stress.",
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T1 - Garlic extract attenuates immobilization stress-induced alterations in plasma antioxidant/oxidant parameters and hepatic function in rats

AU - Zaidi, Syed Kashif

AU - Ansari, Shakeel Ahmed

AU - Tabrez, Shams

AU - Hoda, MD Nasrul

AU - Ashraf, Ghulam Md

AU - Khan, Mohd Shahnawaz

AU - Alnohair, Sultan

AU - Banu, Naheed

AU - Al-Qahtani, Mohammed Hussain

PY - 2017/2/15

Y1 - 2017/2/15

N2 - Objective: To investigate the oxidative stress induced by 6 h of immobilization stress in Albino Wistar rats. Further, the pre- and post-treatment of aqueous garlic extract was studied to evaluate its preventive and curative efficacy on stress-induced altered oxidative parameters in rats. Methods: Albino Wistar rats were exposed to 6 h of immobilization stress, and received garlic extract (100 mg/kg body weight) treatment pre- or post-stress exposure. The oxidative status of plasma after various treatments were evaluated by determining the levels of reduced glutathione, glucose, uric acid, thiobarbituric acid reactive substances, aspartate aminotransferase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and the activities of superoxide dismutase, catalase and glutathione-S-transferase by standardized procedures. Results: Immobilization of rats generated oxidative stress in rat plasma, by decreasing the activities of antioxidant enzymes, glutathione levels and glucose, while increasing the lipid peroxidation, serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, ALP and uric acid compared to the non-stressed controls (P<0.01). The garlic extract administration both pre- and post-stress exposure significantly prevented the rise in the diagnostic liver enzymes and reverted the decrease of antioxidant enzymatic activities compared to the stressed group (P<0.05 or P <0.01). Post-stress treatment of extract was found more effective than pre-stress treatment in reverting the values back to normal (P<0.01). Conclusion: Garlic extract seems promising as a nutritional supplement for scavenging free radicals generated in the plasma and to prevent resulting oxidative stress.

AB - Objective: To investigate the oxidative stress induced by 6 h of immobilization stress in Albino Wistar rats. Further, the pre- and post-treatment of aqueous garlic extract was studied to evaluate its preventive and curative efficacy on stress-induced altered oxidative parameters in rats. Methods: Albino Wistar rats were exposed to 6 h of immobilization stress, and received garlic extract (100 mg/kg body weight) treatment pre- or post-stress exposure. The oxidative status of plasma after various treatments were evaluated by determining the levels of reduced glutathione, glucose, uric acid, thiobarbituric acid reactive substances, aspartate aminotransferase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and the activities of superoxide dismutase, catalase and glutathione-S-transferase by standardized procedures. Results: Immobilization of rats generated oxidative stress in rat plasma, by decreasing the activities of antioxidant enzymes, glutathione levels and glucose, while increasing the lipid peroxidation, serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, ALP and uric acid compared to the non-stressed controls (P<0.01). The garlic extract administration both pre- and post-stress exposure significantly prevented the rise in the diagnostic liver enzymes and reverted the decrease of antioxidant enzymatic activities compared to the stressed group (P<0.05 or P <0.01). Post-stress treatment of extract was found more effective than pre-stress treatment in reverting the values back to normal (P<0.01). Conclusion: Garlic extract seems promising as a nutritional supplement for scavenging free radicals generated in the plasma and to prevent resulting oxidative stress.

KW - alanine transaminase

KW - antioxidant enzymes

KW - aspartate aminotransferase

KW - garlic

KW - malondialdehyde

KW - stress

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