Gastroesophageal reflux-associated aspiration alters the immune response in asthma

Anitra D. Thomas, Kuei Ying Su, Jui Chih Chang, Jason H. Leung, Sean M. Lee, Zoie E. Holzknecht, Mary Lou Everett, William Parker, R. Duane Davis, Shu S. Lin

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background A large number of studies point toward chronic aspiration associated with gastroesophageal reflux disease (GERD) as an important factor involved in the development of asthma, the incidence of which has increased dramatically in industrially developed countries. Recent work suggests that medical intervention aimed at acid blockade is not sufficient to relieve the effects of chronic aspiration on asthma pathology, leaving surgical treatment of the disease as one of the few remaining options. This study examined the effect of chronic aspiration on the airway-associated immune response to allergens using a model of experimentally induced airway hypersensitivity in Balb/c mice. Methods The mice received aspiration of gastric fluid on days 1, 8, 15, 22, 29, 36, 43, and 50 and were sensitized to ovalbumin by intraperitoneal (IP) injection on days 33 and 47, challenged with aerosolized ovalbumin on day 54, and killed on day 56. Control mice received sham gastric fluid aspirations, sham induction of airway hypersensitivity, or both. Results Chronic aspiration of 50 μl murine gastric fluid once per week for 8 weeks had a profound effect on the immune system in the lung, with upregulation of the macrophage/monocyte- associated cytokines tumor necrosis factor-α (TNF-α) and interleukin-12 (IL-12) and profound downregulation of a broad array of T-cell-associated cytokines including interleukins 2, 4, 5, 6, 10, 13, and 23, as well as interferon-γ. The aspiration-induced depression of IL-5 production in particular was found only in mice with airway hypersensitivity and not in control mice without airway hypersensitivity. Conclusions The results indicate that chronic aspiration of gastric fluid has a profound effect on the nature of the allergic response to aerosolized allergens, suggesting that the aspiration may be an important factor affecting the pathogenesis of asthma.

Original languageEnglish (US)
Pages (from-to)1066-1074
Number of pages9
JournalSurgical Endoscopy and Other Interventional Techniques
Volume24
Issue number5
DOIs
StatePublished - May 1 2010

Fingerprint

Gastroesophageal Reflux
Asthma
Stomach
Hypersensitivity
Ovalbumin
Allergens
Cytokines
Surgical Pathology
Interleukin-5
Interleukin-12
Intraperitoneal Injections
Developed Countries
Interleukin-4
Interferons
Interleukin-2
Monocytes
Immune System
Up-Regulation
Down-Regulation
Tumor Necrosis Factor-alpha

Keywords

  • Aspiration
  • Asthma
  • Experimental model
  • Gastroesophageal reflux

ASJC Scopus subject areas

  • Surgery

Cite this

Thomas, A. D., Su, K. Y., Chang, J. C., Leung, J. H., Lee, S. M., Holzknecht, Z. E., ... Lin, S. S. (2010). Gastroesophageal reflux-associated aspiration alters the immune response in asthma. Surgical Endoscopy and Other Interventional Techniques, 24(5), 1066-1074. https://doi.org/10.1007/s00464-009-0727-5

Gastroesophageal reflux-associated aspiration alters the immune response in asthma. / Thomas, Anitra D.; Su, Kuei Ying; Chang, Jui Chih; Leung, Jason H.; Lee, Sean M.; Holzknecht, Zoie E.; Everett, Mary Lou; Parker, William; Davis, R. Duane; Lin, Shu S.

In: Surgical Endoscopy and Other Interventional Techniques, Vol. 24, No. 5, 01.05.2010, p. 1066-1074.

Research output: Contribution to journalArticle

Thomas, AD, Su, KY, Chang, JC, Leung, JH, Lee, SM, Holzknecht, ZE, Everett, ML, Parker, W, Davis, RD & Lin, SS 2010, 'Gastroesophageal reflux-associated aspiration alters the immune response in asthma', Surgical Endoscopy and Other Interventional Techniques, vol. 24, no. 5, pp. 1066-1074. https://doi.org/10.1007/s00464-009-0727-5
Thomas, Anitra D. ; Su, Kuei Ying ; Chang, Jui Chih ; Leung, Jason H. ; Lee, Sean M. ; Holzknecht, Zoie E. ; Everett, Mary Lou ; Parker, William ; Davis, R. Duane ; Lin, Shu S. / Gastroesophageal reflux-associated aspiration alters the immune response in asthma. In: Surgical Endoscopy and Other Interventional Techniques. 2010 ; Vol. 24, No. 5. pp. 1066-1074.
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abstract = "Background A large number of studies point toward chronic aspiration associated with gastroesophageal reflux disease (GERD) as an important factor involved in the development of asthma, the incidence of which has increased dramatically in industrially developed countries. Recent work suggests that medical intervention aimed at acid blockade is not sufficient to relieve the effects of chronic aspiration on asthma pathology, leaving surgical treatment of the disease as one of the few remaining options. This study examined the effect of chronic aspiration on the airway-associated immune response to allergens using a model of experimentally induced airway hypersensitivity in Balb/c mice. Methods The mice received aspiration of gastric fluid on days 1, 8, 15, 22, 29, 36, 43, and 50 and were sensitized to ovalbumin by intraperitoneal (IP) injection on days 33 and 47, challenged with aerosolized ovalbumin on day 54, and killed on day 56. Control mice received sham gastric fluid aspirations, sham induction of airway hypersensitivity, or both. Results Chronic aspiration of 50 μl murine gastric fluid once per week for 8 weeks had a profound effect on the immune system in the lung, with upregulation of the macrophage/monocyte- associated cytokines tumor necrosis factor-α (TNF-α) and interleukin-12 (IL-12) and profound downregulation of a broad array of T-cell-associated cytokines including interleukins 2, 4, 5, 6, 10, 13, and 23, as well as interferon-γ. The aspiration-induced depression of IL-5 production in particular was found only in mice with airway hypersensitivity and not in control mice without airway hypersensitivity. Conclusions The results indicate that chronic aspiration of gastric fluid has a profound effect on the nature of the allergic response to aerosolized allergens, suggesting that the aspiration may be an important factor affecting the pathogenesis of asthma.",
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AU - Lee, Sean M.

AU - Holzknecht, Zoie E.

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