GCSF receptor regulates antigen uptake and expression of cytokines and costimulatory molecules in dendritic cells

Degui Geng, Sunil K. Joshi, Robert Podolsky, Jin Xiong She

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

RNA interference (RNAi), a process that specifically silences target gene expression, is a powerful technique to modulate cellular functions. In this study, we identified two small interference RNA (siRNA) sequences that can specifically and efficiently silence the expression of the granulocyte colony-stimulating factor receptor (GCSF-R) gene and achieved stable knockdown of GCSF-R using pFIV lentivirus containing the GCSF-R siRNA. GCSF-R knockdown significantly reduces the expression of IL-lα, IL-lβ, IL-6, IL-10, H-2Kb, I-Ab, CD80 and CD86, and increases PDL1 and PDL2 expression, while IL-12p35, TGFβ, TNFα and CD40 expression is unaltered. Furthermore, GCSF-R knockdown significantly changes the endocytosis and micro-pinocytosis abilities as well as surface expression of antigens of DC2.4 cells.

Original languageEnglish (US)
Pages (from-to)521-529
Number of pages9
JournalMolecular Immunology
Volume44
Issue number4
DOIs
StatePublished - Jan 1 2007

Keywords

  • Antigen presentation
  • Antigen uptake
  • Dendritic cells
  • GCSF
  • GCSF-R
  • siRNA

ASJC Scopus subject areas

  • Immunology
  • Molecular Biology

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