Gender differences in the expression of endothelin receptors in human saphenous veins in vitro

Adviye Ergul, Kanili Shoemaker, David Puett, Randall L. Tackett

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

The contractile response to endothelin-1 (ET-1) appears to be modulated by the relative density of ET(A) and ET(B) receptors. To determine the effects of gender on the distribution of ET receptors, we analyzed the endothelin receptor subtypes on membrane fractions prepared from saphenous vein samples obtained from patients of different genders undergoing coronary artery bypass graft surgery. The contractile response to ET-1 in the presence and absence of 1 μM of the ET(A) receptor antagonist BQ-123 was also investigated. Similar studies were repeated with endothelium-denuded samples to study the role of endothelium- and smooth muscle-derived ET(B) receptors. Competitive binding experiments were performed on membrane fractions using [125I]ET-1 and unlabeled ligands ET-1, ET-3, sarafatoxin 6c and BQ-123. Analysis of the binding data with endothelium-intact samples yielded two classes of binding sites in both women and men. In women, the maximum binding capacities were 83 ± 6 and 97 ± 10 fmol/mg protein for ET(A) and ET(B) receptors, respectively; the corresponding values in men were 618 ± 121 and 201 ± 10 fmol/mg protein. In addition, ET-1-induced contractions were 2- fold greater in men than in women at high ET-1 concentrations. Competitive binding studies with endothelium-denuded saphenous veins demonstrated the presence of only ETA receptors in both female and male tissue. These results indicate that the ratio and the density of ET receptors are different in men and women, which might be an important factor in the regulation of the contractile response.

Original languageEnglish (US)
Pages (from-to)511-517
Number of pages7
JournalJournal of Pharmacology and Experimental Therapeutics
Volume285
Issue number2
StatePublished - Jan 1 1998

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Endothelin Receptors
Saphenous Vein
Endothelin-1
Endothelium
Competitive Binding
Specific Gravity
Membranes
Coronary Artery Bypass
Smooth Muscle
In Vitro Techniques
Proteins
Binding Sites
Ligands
Transplants

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

Cite this

Gender differences in the expression of endothelin receptors in human saphenous veins in vitro. / Ergul, Adviye; Shoemaker, Kanili; Puett, David; Tackett, Randall L.

In: Journal of Pharmacology and Experimental Therapeutics, Vol. 285, No. 2, 01.01.1998, p. 511-517.

Research output: Contribution to journalArticle

Ergul, Adviye ; Shoemaker, Kanili ; Puett, David ; Tackett, Randall L. / Gender differences in the expression of endothelin receptors in human saphenous veins in vitro. In: Journal of Pharmacology and Experimental Therapeutics. 1998 ; Vol. 285, No. 2. pp. 511-517.
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