Gender differences of renal CYP-derived eicosanoid synthesis in rats fed a high-fat diet

Yiqiang Zhou, Songbai Lin, Hsin Hsin Chang, Juan Du, Zheng Dong, Anne M. Dorrance, Michael W. Brands, Mong Heng Wang

Research output: Contribution to journalArticle

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Abstract

Background: Renal cytochrome P450 (CYP)-derived eicosanoids, hydroxyeicosatetraenoic acids (HETEs), epoxyeicosatrienoic acids (EETs), and dihydroxyeicosatrienoic acids (DHETs), have been shown to affect renal function and blood pressure (BP). We recently reported that high fat (HF) diet treatment in male rats increases BP and decreases production of these eicosanoids in the kidneys. However, at what level the downregulation of renal eicosanoid synthesis occurs and whether the HF diet has any effects on the regulation of renal eicosanoid synthesis in female rats are not known. The purpose of this study was to determine renal CYP-derived eicosanoid synthesis and its association with BP regulation in HF male and female rats. Methods: In the first set of experiments, male and female rats were fed the HF or control diet for 10 weeks. In the second set of experiments, male and female rats were fed the HF diet for 10 days. In the third set of experiments, HF-fed and control female rats were treated with 5α-dihydrotestosterone for 4 weeks. After treatment, BP, urinary sodium, sodium balance, eicosanoid production, and CYP enzyme expression were determined. Results: An elevation of BP and a decrease of renal cortical eicosanoid production were found in HF male rats, but no BP and eicosanoid production changes were observed in HF female rats. The HF treatment also caused a significant decrease of eicosanoid production and a decrease of CYP4A and 2C23 expression in the proximal tubules of HF male rats. Moreover, the HF diet treatment in male rats caused an increase in cumulative sodium balance and an elevation of BP, whereas no change in cumulative sodium balance and BP was observed in female rats. The treatment of 5α-dihydrotestosterone increased BP and 20-HETE production in the renal microvessels, but had no effect on urinary sodium excretion and renal microvessel EET production in both control and HF-fed female rats. Conclusions: These results demonstrate that there are gender-specific differences in regulation of renal eicosanoid synthesis, sodium balance, and BP caused by HF treatment, and it appears that androgens play some role in upregulation of renal microvessel 20-HETE production in both HF and control female rats.

Original languageEnglish (US)
Pages (from-to)530-537
Number of pages8
JournalAmerican journal of hypertension
Volume18
Issue number4
DOIs
StatePublished - Apr 2005

Fingerprint

Eicosanoids
High Fat Diet
Cytochrome P-450 Enzyme System
Kidney
Fats
Blood Pressure
Sodium
Hydroxyeicosatetraenoic Acids
Microvessels
Dihydrotestosterone
Acids
Cytochrome P-450 CYP4A
Androgens
Up-Regulation
Down-Regulation

Keywords

  • 20- Hydroxyeicosatetraenoic acid
  • Arachidonic acid
  • Cytochrome P450
  • Eicosanoid
  • Epoxyeicosatrienoic acid
  • Fat
  • Gender
  • Kidney
  • Obesity

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Gender differences of renal CYP-derived eicosanoid synthesis in rats fed a high-fat diet. / Zhou, Yiqiang; Lin, Songbai; Chang, Hsin Hsin; Du, Juan; Dong, Zheng; Dorrance, Anne M.; Brands, Michael W.; Wang, Mong Heng.

In: American journal of hypertension, Vol. 18, No. 4, 04.2005, p. 530-537.

Research output: Contribution to journalArticle

Zhou, Yiqiang ; Lin, Songbai ; Chang, Hsin Hsin ; Du, Juan ; Dong, Zheng ; Dorrance, Anne M. ; Brands, Michael W. ; Wang, Mong Heng. / Gender differences of renal CYP-derived eicosanoid synthesis in rats fed a high-fat diet. In: American journal of hypertension. 2005 ; Vol. 18, No. 4. pp. 530-537.
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abstract = "Background: Renal cytochrome P450 (CYP)-derived eicosanoids, hydroxyeicosatetraenoic acids (HETEs), epoxyeicosatrienoic acids (EETs), and dihydroxyeicosatrienoic acids (DHETs), have been shown to affect renal function and blood pressure (BP). We recently reported that high fat (HF) diet treatment in male rats increases BP and decreases production of these eicosanoids in the kidneys. However, at what level the downregulation of renal eicosanoid synthesis occurs and whether the HF diet has any effects on the regulation of renal eicosanoid synthesis in female rats are not known. The purpose of this study was to determine renal CYP-derived eicosanoid synthesis and its association with BP regulation in HF male and female rats. Methods: In the first set of experiments, male and female rats were fed the HF or control diet for 10 weeks. In the second set of experiments, male and female rats were fed the HF diet for 10 days. In the third set of experiments, HF-fed and control female rats were treated with 5α-dihydrotestosterone for 4 weeks. After treatment, BP, urinary sodium, sodium balance, eicosanoid production, and CYP enzyme expression were determined. Results: An elevation of BP and a decrease of renal cortical eicosanoid production were found in HF male rats, but no BP and eicosanoid production changes were observed in HF female rats. The HF treatment also caused a significant decrease of eicosanoid production and a decrease of CYP4A and 2C23 expression in the proximal tubules of HF male rats. Moreover, the HF diet treatment in male rats caused an increase in cumulative sodium balance and an elevation of BP, whereas no change in cumulative sodium balance and BP was observed in female rats. The treatment of 5α-dihydrotestosterone increased BP and 20-HETE production in the renal microvessels, but had no effect on urinary sodium excretion and renal microvessel EET production in both control and HF-fed female rats. Conclusions: These results demonstrate that there are gender-specific differences in regulation of renal eicosanoid synthesis, sodium balance, and BP caused by HF treatment, and it appears that androgens play some role in upregulation of renal microvessel 20-HETE production in both HF and control female rats.",
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AU - Zhou, Yiqiang

AU - Lin, Songbai

AU - Chang, Hsin Hsin

AU - Du, Juan

AU - Dong, Zheng

AU - Dorrance, Anne M.

AU - Brands, Michael W.

AU - Wang, Mong Heng

PY - 2005/4

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N2 - Background: Renal cytochrome P450 (CYP)-derived eicosanoids, hydroxyeicosatetraenoic acids (HETEs), epoxyeicosatrienoic acids (EETs), and dihydroxyeicosatrienoic acids (DHETs), have been shown to affect renal function and blood pressure (BP). We recently reported that high fat (HF) diet treatment in male rats increases BP and decreases production of these eicosanoids in the kidneys. However, at what level the downregulation of renal eicosanoid synthesis occurs and whether the HF diet has any effects on the regulation of renal eicosanoid synthesis in female rats are not known. The purpose of this study was to determine renal CYP-derived eicosanoid synthesis and its association with BP regulation in HF male and female rats. Methods: In the first set of experiments, male and female rats were fed the HF or control diet for 10 weeks. In the second set of experiments, male and female rats were fed the HF diet for 10 days. In the third set of experiments, HF-fed and control female rats were treated with 5α-dihydrotestosterone for 4 weeks. After treatment, BP, urinary sodium, sodium balance, eicosanoid production, and CYP enzyme expression were determined. Results: An elevation of BP and a decrease of renal cortical eicosanoid production were found in HF male rats, but no BP and eicosanoid production changes were observed in HF female rats. The HF treatment also caused a significant decrease of eicosanoid production and a decrease of CYP4A and 2C23 expression in the proximal tubules of HF male rats. Moreover, the HF diet treatment in male rats caused an increase in cumulative sodium balance and an elevation of BP, whereas no change in cumulative sodium balance and BP was observed in female rats. The treatment of 5α-dihydrotestosterone increased BP and 20-HETE production in the renal microvessels, but had no effect on urinary sodium excretion and renal microvessel EET production in both control and HF-fed female rats. Conclusions: These results demonstrate that there are gender-specific differences in regulation of renal eicosanoid synthesis, sodium balance, and BP caused by HF treatment, and it appears that androgens play some role in upregulation of renal microvessel 20-HETE production in both HF and control female rats.

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KW - Arachidonic acid

KW - Cytochrome P450

KW - Eicosanoid

KW - Epoxyeicosatrienoic acid

KW - Fat

KW - Gender

KW - Kidney

KW - Obesity

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