Gender-specific differential expression of exosomal miRNA in synovial fluid of patients with osteoarthritis

Ravindra Bharat Kolhe, David M Hunter, Siyang Liu, Ravirajsinh N. Jadeja, Chetan Pundkar, Ashis K. Mondal, Bharati Mendhe, Michelle Drewry, Mumtaz V Rojiani, Yutao Liu, Carlos M Isales, Robert E. Guldberg, Mark W Hamrick, Sadanand T Fulzele

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The pathogenesis of osteoarthritis (OA) is poorly understood, and therapeutic approaches are limited to preventing progression of the disease. Recent studies have shown that exosomes play a vital role in cell-to-cell communication, and pathogenesis of many age-related diseases. Molecular profiling of synovial fluid derived exosomal miRNAs may increase our understanding of OA progression and may lead to the discovery of novel biomarkers and therapeutic targets. In this article we report the first characterization of exosomes miRNAs from human synovial fluid. The synovial fluid exosomes share similar characteristics (size, surface marker, miRNA content) with previously described exosomes in other body fluids. MiRNA microarray analysis showed OA specific exosomal miRNA of male and female OA. Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis identified gender-specific target genes/signaling pathways. These pathway analyses showed that female OA specific miRNAs are estrogen responsive and target TLR (toll-like receptor) signaling pathways. Furthermore, articular chondrocytes treated with OA derived extracellular vesicles had decreased expression of anabolic genes and elevated expression of catabolic and inflammatory genes. In conclusion, synovial fluid exosomal miRNA content is altered in patients with OA and these changes are gender specific.

Original languageEnglish (US)
Article number2029
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

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Synovial Fluid
MicroRNAs
Osteoarthritis
Exosomes
Encyclopedias
Genes
Gene Ontology
Toll-Like Receptors
Body Fluids
Microarray Analysis
Chondrocytes
Cell Communication
Disease Progression
Estrogens
Joints
Biomarkers
Genome
Gene Expression
Therapeutics

ASJC Scopus subject areas

  • General

Cite this

Gender-specific differential expression of exosomal miRNA in synovial fluid of patients with osteoarthritis. / Kolhe, Ravindra Bharat; Hunter, David M; Liu, Siyang; Jadeja, Ravirajsinh N.; Pundkar, Chetan; Mondal, Ashis K.; Mendhe, Bharati; Drewry, Michelle; Rojiani, Mumtaz V; Liu, Yutao; Isales, Carlos M; Guldberg, Robert E.; Hamrick, Mark W; Fulzele, Sadanand T.

In: Scientific Reports, Vol. 7, No. 1, 2029, 01.12.2017.

Research output: Contribution to journalArticle

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