Gene expression in the developing mouse retina by EST sequencing and microarray analysis

Xiuqian Mu, Sheng Zhao, Rashmi Pershad, Tzung Fu Hsieh, Ann Scarpa, Steven W. Wang, R. Allen White, Phillip D. Beremand, Terry L. Thomas, Lin Gan, William H. Klein

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

Retinal development occurs in mice between embryonic day E11.5 and post-natal day P8 as uncommitted neuroblasts assume retinal cell fates. The genetic pathways regulating retinal development are being identified but little is understood about the global networks that link these pathways together or the complexity of the expressed gene set required to form the retina. At E14.5, the retina contains mostly uncommitted neuroblasts and newly differentiated neurons. Here we report a sequence analysis of an E14.5 retinal cDNA library. To date, we have archived 15 268 ESTs and have annotated 9035, which represent 5288 genes. The fraction of singly occurring ESTs as a function of total EST accrual suggests that the total number of expressed genes in the library could approach 27 000. The 9035 ESTs were categorized by their known or putative functions. Representation of the genes involved in eye development was significantly higher in the retinal clone set compared with the NIA mouse 15K cDNA clone set. Screening with a microarray containing 864 cDNA clones using wild-type and brn-3b (-/-) retinal cDNA probes revealed a potential regulatory linkage between the transcription factor Brn-3b and expression of GAP-43, a protein associated with axon growth. The retinal EST database will be a valuable platform for gene expression profiling and a new source for gene discovery.

Original languageEnglish (US)
Pages (from-to)4983-4993
Number of pages11
JournalNucleic Acids Research
Volume29
Issue number24
DOIs
StatePublished - Dec 24 2001

ASJC Scopus subject areas

  • Genetics

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    Mu, X., Zhao, S., Pershad, R., Hsieh, T. F., Scarpa, A., Wang, S. W., White, R. A., Beremand, P. D., Thomas, T. L., Gan, L., & Klein, W. H. (2001). Gene expression in the developing mouse retina by EST sequencing and microarray analysis. Nucleic Acids Research, 29(24), 4983-4993. https://doi.org/10.1093/nar/29.24.4983