Gene Expression of Cytokine Receptors in HL60 Cells Exposed to a 50 Hz Magnetic Field

Jiliang Zhou, Changlin Li, Gengdong Yao, Huai Chiang, Zongliang Chang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The effects of a 50 Hz extremely low frequency (ELF) sinusoidal magnetic field (MF) on the expression of genes relating to cytokine receptors were studied in HL60 cells. Transcription levels of tumor necrosis factor receptor (TNFR) p55 and p75, interleukin-6 receptor-α (IL-6Rα) and transforming growth factor-β receptor 1 (TGFβR1) were quantified in cells exposed to an intensity of 0.1 or 0.8 mT for periods ranging from 30 min to 72 h. Cells treated with 10 nM of phorbol 12-myristate 13-acetate (PMA) for 8 h served as a positive control. Gene expression values were assessed by the ribonuclease protection assay (RPA) and normalized to those of the noninducible gene GAPDH. The results showed that MF exposure at 0.1 and 0.8 mT for 72 h increased TNFR p75 and IL-6Rα mRNA expression in HL60 cells. No significant change in gene expression levels of TNFR p55 and TGFβR1 was observed under any of the exposure conditions. In addition, we report here for the first time that IL-6Rα mRNA expression can be suppressed by PMA in HL60 cells.

Original languageEnglish (US)
Pages (from-to)339-346
Number of pages8
JournalBioelectromagnetics
Volume23
Issue number5
DOIs
StatePublished - Jul 1 2002
Externally publishedYes

Fingerprint

Interleukin-6 Receptors
Cytokine Receptors
Tumor Necrosis Factor Receptors
HL-60 Cells
Magnetic Fields
Growth Factor Receptors
Transforming Growth Factors
Gene Expression
Acetates
Messenger RNA
Ribonucleases
Genes
phorbol-12-myristate

Keywords

  • IL-6 receptor
  • Interleukin-6
  • TGFβ receptor
  • TNF receptor
  • Transforming growth factor
  • Tumor necrosis factor

ASJC Scopus subject areas

  • Biophysics
  • Physiology
  • Radiology Nuclear Medicine and imaging

Cite this

Gene Expression of Cytokine Receptors in HL60 Cells Exposed to a 50 Hz Magnetic Field. / Zhou, Jiliang; Li, Changlin; Yao, Gengdong; Chiang, Huai; Chang, Zongliang.

In: Bioelectromagnetics, Vol. 23, No. 5, 01.07.2002, p. 339-346.

Research output: Contribution to journalArticle

Zhou, Jiliang ; Li, Changlin ; Yao, Gengdong ; Chiang, Huai ; Chang, Zongliang. / Gene Expression of Cytokine Receptors in HL60 Cells Exposed to a 50 Hz Magnetic Field. In: Bioelectromagnetics. 2002 ; Vol. 23, No. 5. pp. 339-346.
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