Abstract
Liver is a quiescent organ with >90% of the cells present in the G 0 stage of the cell cycle. However, adult hepatocytes have enormous ability to proliferate in response to liver injury. After 70% liver resection hepatocytes enter the cell cycle in a highly synchronized manner and undergo 1 to 2 rounds of cell division to restore the lost organ mass, thus, representing one of the most reliable model systems to study cell cycle progression in vivo. Using high density oligonucleotide micro-array we analyzed the expression patterns of genes at the G1/S transition of liver regeneration in comparison to quiescent livers. The G1/S boundary was identified by in vivo BrdU pulse labeling and we observed 199 genes/ESTs which were either up/down regulated at this time point. These differentially regulated genes have a wide range of functions including transcriptional regulation, signal transduction, cell cycle regulation, chromatin reorganization, protein targeting, metabolism, transport, surface receptors, circadian rhythms, xenobiotic metabolism, inflammation and acute phase response. The functions of most of the genes identified in this screen are not known in the process of liver regeneration and cell cycle control at G1/S transition.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1405-1417 |
| Number of pages | 13 |
| Journal | Cell Cycle |
| Volume | 3 |
| Issue number | 11 |
| DOIs | |
| State | Published - Jan 1 2004 |
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Keywords
- Affymetrix
- Cell cycle
- G/S transition
- Liver regeneration
- Microarray
- PH
ASJC Scopus subject areas
- Molecular Biology
- Developmental Biology
- Cell Biology
Cite this
Gene expression profile at the G1/S transition of liver regeneration after partial hepatectomy in mice. / Ande, Satyanarayana; Geffers, Robert; Manns, Michael P.; Buer, Jan; Rudolph, K. Lenhard.
In: Cell Cycle, Vol. 3, No. 11, 01.01.2004, p. 1405-1417.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Gene expression profile at the G1/S transition of liver regeneration after partial hepatectomy in mice
AU - Ande, Satyanarayana
AU - Geffers, Robert
AU - Manns, Michael P.
AU - Buer, Jan
AU - Rudolph, K. Lenhard
PY - 2004/1/1
Y1 - 2004/1/1
N2 - Liver is a quiescent organ with >90% of the cells present in the G 0 stage of the cell cycle. However, adult hepatocytes have enormous ability to proliferate in response to liver injury. After 70% liver resection hepatocytes enter the cell cycle in a highly synchronized manner and undergo 1 to 2 rounds of cell division to restore the lost organ mass, thus, representing one of the most reliable model systems to study cell cycle progression in vivo. Using high density oligonucleotide micro-array we analyzed the expression patterns of genes at the G1/S transition of liver regeneration in comparison to quiescent livers. The G1/S boundary was identified by in vivo BrdU pulse labeling and we observed 199 genes/ESTs which were either up/down regulated at this time point. These differentially regulated genes have a wide range of functions including transcriptional regulation, signal transduction, cell cycle regulation, chromatin reorganization, protein targeting, metabolism, transport, surface receptors, circadian rhythms, xenobiotic metabolism, inflammation and acute phase response. The functions of most of the genes identified in this screen are not known in the process of liver regeneration and cell cycle control at G1/S transition.
AB - Liver is a quiescent organ with >90% of the cells present in the G 0 stage of the cell cycle. However, adult hepatocytes have enormous ability to proliferate in response to liver injury. After 70% liver resection hepatocytes enter the cell cycle in a highly synchronized manner and undergo 1 to 2 rounds of cell division to restore the lost organ mass, thus, representing one of the most reliable model systems to study cell cycle progression in vivo. Using high density oligonucleotide micro-array we analyzed the expression patterns of genes at the G1/S transition of liver regeneration in comparison to quiescent livers. The G1/S boundary was identified by in vivo BrdU pulse labeling and we observed 199 genes/ESTs which were either up/down regulated at this time point. These differentially regulated genes have a wide range of functions including transcriptional regulation, signal transduction, cell cycle regulation, chromatin reorganization, protein targeting, metabolism, transport, surface receptors, circadian rhythms, xenobiotic metabolism, inflammation and acute phase response. The functions of most of the genes identified in this screen are not known in the process of liver regeneration and cell cycle control at G1/S transition.
KW - Affymetrix
KW - Cell cycle
KW - G/S transition
KW - Liver regeneration
KW - Microarray
KW - PH
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UR - http://www.scopus.com/inward/citedby.url?scp=14644404862&partnerID=8YFLogxK
U2 - 10.4161/cc.3.11.1212
DO - 10.4161/cc.3.11.1212
M3 - Article
C2 - 15492508
AN - SCOPUS:14644404862
VL - 3
SP - 1405
EP - 1417
JO - Cell Cycle
JF - Cell Cycle
SN - 1538-4101
IS - 11
ER -