Gene expression profile at the G1/S transition of liver regeneration after partial hepatectomy in mice

Satyanarayana Ande, Robert Geffers, Michael P. Manns, Jan Buer, K. Lenhard Rudolph

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Liver is a quiescent organ with >90% of the cells present in the G 0 stage of the cell cycle. However, adult hepatocytes have enormous ability to proliferate in response to liver injury. After 70% liver resection hepatocytes enter the cell cycle in a highly synchronized manner and undergo 1 to 2 rounds of cell division to restore the lost organ mass, thus, representing one of the most reliable model systems to study cell cycle progression in vivo. Using high density oligonucleotide micro-array we analyzed the expression patterns of genes at the G1/S transition of liver regeneration in comparison to quiescent livers. The G1/S boundary was identified by in vivo BrdU pulse labeling and we observed 199 genes/ESTs which were either up/down regulated at this time point. These differentially regulated genes have a wide range of functions including transcriptional regulation, signal transduction, cell cycle regulation, chromatin reorganization, protein targeting, metabolism, transport, surface receptors, circadian rhythms, xenobiotic metabolism, inflammation and acute phase response. The functions of most of the genes identified in this screen are not known in the process of liver regeneration and cell cycle control at G1/S transition.

Original languageEnglish (US)
Pages (from-to)1405-1417
Number of pages13
JournalCell Cycle
Volume3
Issue number11
DOIs
StatePublished - Jan 1 2004

Fingerprint

Liver Regeneration
Hepatectomy
Transcriptome
Cell Cycle
Liver
Hepatocytes
Genes
Acute-Phase Reaction
Expressed Sequence Tags
Xenobiotics
Protein Transport
Bromodeoxyuridine
Circadian Rhythm
Cell Cycle Checkpoints
Oligonucleotide Array Sequence Analysis
Cell Division
Chromatin
Signal Transduction
Inflammation
Gene Expression

Keywords

  • Affymetrix
  • Cell cycle
  • G/S transition
  • Liver regeneration
  • Microarray
  • PH

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Gene expression profile at the G1/S transition of liver regeneration after partial hepatectomy in mice. / Ande, Satyanarayana; Geffers, Robert; Manns, Michael P.; Buer, Jan; Rudolph, K. Lenhard.

In: Cell Cycle, Vol. 3, No. 11, 01.01.2004, p. 1405-1417.

Research output: Contribution to journalArticle

Ande, Satyanarayana ; Geffers, Robert ; Manns, Michael P. ; Buer, Jan ; Rudolph, K. Lenhard. / Gene expression profile at the G1/S transition of liver regeneration after partial hepatectomy in mice. In: Cell Cycle. 2004 ; Vol. 3, No. 11. pp. 1405-1417.
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