Gene expression profiling during all-trans retinoic acid-induced cell differentiation of acute promyelocytic leukemia cells

Lijun Yang, Hongshan Zhao, Shi Wu Li, Kim Ahrens, Christine Collins, Sarah Eckenrode, Qing Guo Ruan, Richard A McIndoe, Jin-Xiong She

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Using cDNA microarrays we determined the gene expression patterns in the human acute promyelocytic leukemia (APL) cell line NB4 during all-trans retinoic acid (ATRA)-induced differentiation. We analyzed the expression of 12,288 genes in the NB4 cells after 12 hours, 24 hours, 48 hours, 72 hours, and 96 hours of ATRA exposure. During this time course, we found 168 up-regulated and more than 179 down-regulated genes, most of which have not been reported before. Many of the altered genes encode products that participate in signaling pathways, cell differentiation, programmed cell death, transcription regulation, and production of cytokines and chemokines. Of interest, the CD52 and protein kinase A regulatory subunit α (PKA-R1α) genes, whose products are being used as therapeutic targets for certain human neoplasias in currently ongoing clinical trials, were among the genes observed to be markedly up-regulated after ATRA treatment. The present study provides valuable data to further understand the mechanism of ATRA-induced APL cell differentiation and suggests potential therapeutic alternatives for this leukemia.

Original languageEnglish (US)
Pages (from-to)212-221
Number of pages10
JournalJournal of Molecular Diagnostics
Volume5
Issue number4
DOIs
StatePublished - Jan 1 2003

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Acute Promyelocytic Leukemia
Gene Expression Profiling
Tretinoin
Cell Differentiation
Genes
Cyclic AMP-Dependent Protein Kinases
Oligonucleotide Array Sequence Analysis
Chemokines
Leukemia
Cell Death
Clinical Trials
Cytokines
Gene Expression
Cell Line
Therapeutics
Neoplasms

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Medicine

Cite this

Gene expression profiling during all-trans retinoic acid-induced cell differentiation of acute promyelocytic leukemia cells. / Yang, Lijun; Zhao, Hongshan; Li, Shi Wu; Ahrens, Kim; Collins, Christine; Eckenrode, Sarah; Ruan, Qing Guo; McIndoe, Richard A; She, Jin-Xiong.

In: Journal of Molecular Diagnostics, Vol. 5, No. 4, 01.01.2003, p. 212-221.

Research output: Contribution to journalArticle

Yang, Lijun ; Zhao, Hongshan ; Li, Shi Wu ; Ahrens, Kim ; Collins, Christine ; Eckenrode, Sarah ; Ruan, Qing Guo ; McIndoe, Richard A ; She, Jin-Xiong. / Gene expression profiling during all-trans retinoic acid-induced cell differentiation of acute promyelocytic leukemia cells. In: Journal of Molecular Diagnostics. 2003 ; Vol. 5, No. 4. pp. 212-221.
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