Gene structure and chromosomal mapping of the rat smooth muscle calponin gene

Marcelo A. Nobrega, Masahide Shiozawa, George Koike, Howard J. Jacob, Joseph M. Miano

Research output: Contribution to journalArticle

Abstract

Smooth muscle cells (SMC) express a battery of lineage-restricted genes whose encoded proteins impart the unique contractile phenotype that characterizes this muscle type. While the encoded function of many SMC- restricted genes has been extensively analyzed, less is known about their position within the genome and the regulatory factors governing their transcription. In this report, we define the gene structure, 5' promoter analysis, and chromosomal mapping of the rat smooth muscle calponin (CnnI) gene. The rat CnnI gene is comprised of seven exons spanning approximately 8 kb of genomic sequence. The intron-exon boundaries of the rat CnnI gene match precisely those in human and mouse. Primer extension and RNase protection assays indicate two major transcription start positions (tsp). Comparative sequence analysis of the 5' promoter region reveals several conserved cis regulatory elements, including a TA-rich element within 30 nt of the tsp that could be a recognition site for TATA-binding protein and two CCAAT boxes. Transient and stable transfection studies support the hypothesis that distal regulatory elements confer SMC-restricted expression of CnnI. Finally, using an F2 intercross, we have mapped the rat CnnI gene to the telomeric end of Chromosome (Chr) 8. These studies provide additional information relating to the control of CnnI gene expression and provide a platform to begin assessing the potential linkage of CnnI to spontaneous and experimental disease phenotypes in rats.

Original languageEnglish (US)
Pages (from-to)115-119
Number of pages5
JournalMammalian Genome
Volume11
Issue number2
DOIs
StatePublished - Jan 29 2000
Externally publishedYes

Fingerprint

Smooth Muscle
Genes
Smooth Muscle Myocytes
Exons
TATA-Box Binding Protein
Phenotype
Chromosomes, Human, Pair 8
Ribonucleases
calponin
Genetic Promoter Regions
Introns
Transfection
Sequence Analysis
Genome
Gene Expression
Muscles
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Nobrega, M. A., Shiozawa, M., Koike, G., Jacob, H. J., & Miano, J. M. (2000). Gene structure and chromosomal mapping of the rat smooth muscle calponin gene. Mammalian Genome, 11(2), 115-119. https://doi.org/10.1007/s003350010023

Gene structure and chromosomal mapping of the rat smooth muscle calponin gene. / Nobrega, Marcelo A.; Shiozawa, Masahide; Koike, George; Jacob, Howard J.; Miano, Joseph M.

In: Mammalian Genome, Vol. 11, No. 2, 29.01.2000, p. 115-119.

Research output: Contribution to journalArticle

Nobrega, MA, Shiozawa, M, Koike, G, Jacob, HJ & Miano, JM 2000, 'Gene structure and chromosomal mapping of the rat smooth muscle calponin gene', Mammalian Genome, vol. 11, no. 2, pp. 115-119. https://doi.org/10.1007/s003350010023
Nobrega, Marcelo A. ; Shiozawa, Masahide ; Koike, George ; Jacob, Howard J. ; Miano, Joseph M. / Gene structure and chromosomal mapping of the rat smooth muscle calponin gene. In: Mammalian Genome. 2000 ; Vol. 11, No. 2. pp. 115-119.
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