Generation and characterization of Lhx3GFP reporter knockin and Lhx3loxP conditional knockout mice

Mei Xu, Xiaoling Xie, Xuhui Dong, Guoqing Liang, Lin Gan

Research output: Contribution to journalArticle

Abstract

LHX3, a LIM-homeodomain transcription factor, is broadly expressed in the developing pituitary, spinal cord, medulla, retina and inner ear, and plays essential roles during embryonic development. Mice with homozygous Lhx3 null mutation exhibit failure in the formation of pituitary gland and die perinatally. To facilitate the functional study of Lhx3 in mice, we engineered and characterized two novel Lhx3 mouse strains: Lhx3GFP reporter knock-in and Lhx3loxP conditional knockout mice. Coimmunolabeling of LHX3 and GFP shows that the expression pattern of the knock-in GFP reporter recapitulates that of endogenous LHX3 in cochlea, vestibule, retina, and spinal cord. By crossing Lhx3loxP mice with the ubiquitous CMV-Cre mice, we have demonstrated a high efficiency of Cre recombinase-mediated removal of exons 3 to 5 of Lhx3, which encode the second LIM-domain and the HD domain of LHX3, resulting global knockout of Lhx3. Thus, Lhx3GFP and Lhx3loxP mice serve as valuable genetic tools to dissect the tissue-specific roles of Lhx3 at late-gestation and postnatal stages in mice.

Original languageEnglish (US)
Article numbere23098
JournalGenesis
Volume56
Issue number4
DOIs
StatePublished - Apr 2018

Fingerprint

Knockout Mice
Retina
Spinal Cord
Cochlea
Inner Ear
Pituitary Gland
Embryonic Development
Exons
Transcription Factors
Pregnancy
Mutation

Keywords

  • LIM homeodomain
  • inner ear
  • pituitary
  • retina
  • spinal cord
  • transcription factor

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

Cite this

Generation and characterization of Lhx3GFP reporter knockin and Lhx3loxP conditional knockout mice. / Xu, Mei; Xie, Xiaoling; Dong, Xuhui; Liang, Guoqing; Gan, Lin.

In: Genesis, Vol. 56, No. 4, e23098, 04.2018.

Research output: Contribution to journalArticle

Xu, Mei ; Xie, Xiaoling ; Dong, Xuhui ; Liang, Guoqing ; Gan, Lin. / Generation and characterization of Lhx3GFP reporter knockin and Lhx3loxP conditional knockout mice. In: Genesis. 2018 ; Vol. 56, No. 4.
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