Generation and characterization of Lhx4tdT reporter knock-in and Lhx4loxP conditional knockout mice

Xuhui Dong, Xiaoling Xie, Luming Guo, Jiadong Xu, Mei Xu, Guoqing Liang, Lin Gan

Research output: Contribution to journalArticle

Abstract

LHX4 is a LIM-homeodomain transcription factor essential for the development of spinal cord and pituitary gland. Mice with homozygous Lhx4-null mutation suffer early postnatal death from lung defect. In this study, to facilitate the research on Lhx4 function, we designed a targeting construct to generate two novel Lhx4 mouse lines: Lhx4loxP conditional knockout and Lhx4tdT reporter knock-in mice. Lhx4tdT/+, Lhx4loxP/+, and Lhx4loxP/loxP were viable, fertile, and did not display any gross abnormalities. By breeding Lhx4loxP line with Cre-expressing mice, the Exon 3 of Lhx4 was efficiently removed, resulting in a shift in the reading frame and the inactivation of Lhx4. The expression of tdTomato knock-in reporter recapitulated the endogenous LHX4 expression and was detected in the retina, spinal cord, pituitary gland, and hindbrain of Lhx4tdT mice. Thus, Lhx4tdT and Lhx4loxP mouse lines provide valuable tools for unraveling the tissue-specific role of Lhx4 at postnatal stages in mice.

Original languageEnglish (US)
Article numbere23328
JournalGenesis
Volume57
Issue number10
DOIs
StatePublished - Oct 1 2019

Fingerprint

Knockout Mice
Pituitary Gland
Spinal Cord
Reading Frames
Rhombencephalon
Breeding
Retina
Exons
Transcription Factors
Lung
Mutation
Research

Keywords

  • LIM-homeodomain
  • homeobox
  • pituitary gland
  • retina
  • spinal cord

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

Cite this

Dong, X., Xie, X., Guo, L., Xu, J., Xu, M., Liang, G., & Gan, L. (2019). Generation and characterization of Lhx4tdT reporter knock-in and Lhx4loxP conditional knockout mice. Genesis, 57(10), [e23328]. https://doi.org/10.1002/dvg.23328

Generation and characterization of Lhx4tdT reporter knock-in and Lhx4loxP conditional knockout mice. / Dong, Xuhui; Xie, Xiaoling; Guo, Luming; Xu, Jiadong; Xu, Mei; Liang, Guoqing; Gan, Lin.

In: Genesis, Vol. 57, No. 10, e23328, 01.10.2019.

Research output: Contribution to journalArticle

Dong, X, Xie, X, Guo, L, Xu, J, Xu, M, Liang, G & Gan, L 2019, 'Generation and characterization of Lhx4tdT reporter knock-in and Lhx4loxP conditional knockout mice', Genesis, vol. 57, no. 10, e23328. https://doi.org/10.1002/dvg.23328
Dong, Xuhui ; Xie, Xiaoling ; Guo, Luming ; Xu, Jiadong ; Xu, Mei ; Liang, Guoqing ; Gan, Lin. / Generation and characterization of Lhx4tdT reporter knock-in and Lhx4loxP conditional knockout mice. In: Genesis. 2019 ; Vol. 57, No. 10.
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