Generation and characterization of Lhx9-GFPCreERT2 knock-in mouse line

Revathi Balasubramanian, Andrew Bui, Xiaoling Xie, Min Deng, Lin Gan

Research output: Contribution to journalArticle

Abstract

LHX9 is a LIM-homeodomain transcription factor essential for the development of gonads, spinal cord interneurons, and thalamic neurons to name a few. We recently reported the expression of LHX9 in retinal amacrine cells during development. In this study, we generated an Lhx9-GFPCreERT2 (GCE) knock-in mouse line by knocking-in a GCE cassette at the Lhx9 locus, thus inactivating endogenous Lhx9. Lhx9GCE/+ mice were viable, fertile, and displayed no overt phenotypical characteristics. Lhx9GCE/GCE mice were all phenotypically female, smaller in size, viable, but infertile. The specificity and efficacy of the Lhx9-GCE mouse line was verified by crossing it to a Rosa26-tdTomato reporter mouse line, which reveals the Cre recombinase activities in retinal amacrine cells, developing limbs, testis, hippocampal neurons, thalamic neurons, and cerebellar neurons. Taken together, the Lhx9-GCE mouse line could serve as a beneficial tool for lineage tracing and gene manipulation experiments.

Original languageEnglish (US)
Pages (from-to)827-832
Number of pages6
JournalGenesis
Volume52
Issue number9
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

Fingerprint

Amacrine Cells
Neurons
Gonads
Interneurons
Names
Testis
Spinal Cord
Transcription Factors
Extremities
Genes
Cre recombinase

Keywords

  • Amacrine cells
  • Cre recombinase
  • Lhx9
  • LIM-homeodomain
  • Retina
  • Transcription factor

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

Cite this

Balasubramanian, R., Bui, A., Xie, X., Deng, M., & Gan, L. (2014). Generation and characterization of Lhx9-GFPCreERT2 knock-in mouse line. Genesis, 52(9), 827-832. https://doi.org/10.1002/dvg.22805

Generation and characterization of Lhx9-GFPCreERT2 knock-in mouse line. / Balasubramanian, Revathi; Bui, Andrew; Xie, Xiaoling; Deng, Min; Gan, Lin.

In: Genesis, Vol. 52, No. 9, 01.01.2014, p. 827-832.

Research output: Contribution to journalArticle

Balasubramanian, R, Bui, A, Xie, X, Deng, M & Gan, L 2014, 'Generation and characterization of Lhx9-GFPCreERT2 knock-in mouse line', Genesis, vol. 52, no. 9, pp. 827-832. https://doi.org/10.1002/dvg.22805
Balasubramanian R, Bui A, Xie X, Deng M, Gan L. Generation and characterization of Lhx9-GFPCreERT2 knock-in mouse line. Genesis. 2014 Jan 1;52(9):827-832. https://doi.org/10.1002/dvg.22805
Balasubramanian, Revathi ; Bui, Andrew ; Xie, Xiaoling ; Deng, Min ; Gan, Lin. / Generation and characterization of Lhx9-GFPCreERT2 knock-in mouse line. In: Genesis. 2014 ; Vol. 52, No. 9. pp. 827-832.
@article{b1463c9c7e144e7fb845403b641cd482,
title = "Generation and characterization of Lhx9-GFPCreERT2 knock-in mouse line",
abstract = "LHX9 is a LIM-homeodomain transcription factor essential for the development of gonads, spinal cord interneurons, and thalamic neurons to name a few. We recently reported the expression of LHX9 in retinal amacrine cells during development. In this study, we generated an Lhx9-GFPCreERT2 (GCE) knock-in mouse line by knocking-in a GCE cassette at the Lhx9 locus, thus inactivating endogenous Lhx9. Lhx9GCE/+ mice were viable, fertile, and displayed no overt phenotypical characteristics. Lhx9GCE/GCE mice were all phenotypically female, smaller in size, viable, but infertile. The specificity and efficacy of the Lhx9-GCE mouse line was verified by crossing it to a Rosa26-tdTomato reporter mouse line, which reveals the Cre recombinase activities in retinal amacrine cells, developing limbs, testis, hippocampal neurons, thalamic neurons, and cerebellar neurons. Taken together, the Lhx9-GCE mouse line could serve as a beneficial tool for lineage tracing and gene manipulation experiments.",
keywords = "Amacrine cells, Cre recombinase, Lhx9, LIM-homeodomain, Retina, Transcription factor",
author = "Revathi Balasubramanian and Andrew Bui and Xiaoling Xie and Min Deng and Lin Gan",
year = "2014",
month = "1",
day = "1",
doi = "10.1002/dvg.22805",
language = "English (US)",
volume = "52",
pages = "827--832",
journal = "Genesis",
issn = "1526-954X",
publisher = "Wiley-Liss Inc.",
number = "9",

}

TY - JOUR

T1 - Generation and characterization of Lhx9-GFPCreERT2 knock-in mouse line

AU - Balasubramanian, Revathi

AU - Bui, Andrew

AU - Xie, Xiaoling

AU - Deng, Min

AU - Gan, Lin

PY - 2014/1/1

Y1 - 2014/1/1

N2 - LHX9 is a LIM-homeodomain transcription factor essential for the development of gonads, spinal cord interneurons, and thalamic neurons to name a few. We recently reported the expression of LHX9 in retinal amacrine cells during development. In this study, we generated an Lhx9-GFPCreERT2 (GCE) knock-in mouse line by knocking-in a GCE cassette at the Lhx9 locus, thus inactivating endogenous Lhx9. Lhx9GCE/+ mice were viable, fertile, and displayed no overt phenotypical characteristics. Lhx9GCE/GCE mice were all phenotypically female, smaller in size, viable, but infertile. The specificity and efficacy of the Lhx9-GCE mouse line was verified by crossing it to a Rosa26-tdTomato reporter mouse line, which reveals the Cre recombinase activities in retinal amacrine cells, developing limbs, testis, hippocampal neurons, thalamic neurons, and cerebellar neurons. Taken together, the Lhx9-GCE mouse line could serve as a beneficial tool for lineage tracing and gene manipulation experiments.

AB - LHX9 is a LIM-homeodomain transcription factor essential for the development of gonads, spinal cord interneurons, and thalamic neurons to name a few. We recently reported the expression of LHX9 in retinal amacrine cells during development. In this study, we generated an Lhx9-GFPCreERT2 (GCE) knock-in mouse line by knocking-in a GCE cassette at the Lhx9 locus, thus inactivating endogenous Lhx9. Lhx9GCE/+ mice were viable, fertile, and displayed no overt phenotypical characteristics. Lhx9GCE/GCE mice were all phenotypically female, smaller in size, viable, but infertile. The specificity and efficacy of the Lhx9-GCE mouse line was verified by crossing it to a Rosa26-tdTomato reporter mouse line, which reveals the Cre recombinase activities in retinal amacrine cells, developing limbs, testis, hippocampal neurons, thalamic neurons, and cerebellar neurons. Taken together, the Lhx9-GCE mouse line could serve as a beneficial tool for lineage tracing and gene manipulation experiments.

KW - Amacrine cells

KW - Cre recombinase

KW - Lhx9

KW - LIM-homeodomain

KW - Retina

KW - Transcription factor

UR - http://www.scopus.com/inward/record.url?scp=84927574650&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84927574650&partnerID=8YFLogxK

U2 - 10.1002/dvg.22805

DO - 10.1002/dvg.22805

M3 - Article

C2 - 25112520

AN - SCOPUS:84927574650

VL - 52

SP - 827

EP - 832

JO - Genesis

JF - Genesis

SN - 1526-954X

IS - 9

ER -