TY - JOUR
T1 - Generation and characterization of Lhx9-GFPCreERT2 knock-in mouse line
AU - Balasubramanian, Revathi
AU - Bui, Andrew
AU - Xie, Xiaoling
AU - Deng, Min
AU - Gan, Lin
N1 - Publisher Copyright:
© 2014 Wiley Periodicals, Inc.
PY - 2014/9
Y1 - 2014/9
N2 - LHX9 is a LIM-homeodomain transcription factor essential for the development of gonads, spinal cord interneurons, and thalamic neurons to name a few. We recently reported the expression of LHX9 in retinal amacrine cells during development. In this study, we generated an Lhx9-GFPCreERT2 (GCE) knock-in mouse line by knocking-in a GCE cassette at the Lhx9 locus, thus inactivating endogenous Lhx9. Lhx9GCE/+ mice were viable, fertile, and displayed no overt phenotypical characteristics. Lhx9GCE/GCE mice were all phenotypically female, smaller in size, viable, but infertile. The specificity and efficacy of the Lhx9-GCE mouse line was verified by crossing it to a Rosa26-tdTomato reporter mouse line, which reveals the Cre recombinase activities in retinal amacrine cells, developing limbs, testis, hippocampal neurons, thalamic neurons, and cerebellar neurons. Taken together, the Lhx9-GCE mouse line could serve as a beneficial tool for lineage tracing and gene manipulation experiments.
AB - LHX9 is a LIM-homeodomain transcription factor essential for the development of gonads, spinal cord interneurons, and thalamic neurons to name a few. We recently reported the expression of LHX9 in retinal amacrine cells during development. In this study, we generated an Lhx9-GFPCreERT2 (GCE) knock-in mouse line by knocking-in a GCE cassette at the Lhx9 locus, thus inactivating endogenous Lhx9. Lhx9GCE/+ mice were viable, fertile, and displayed no overt phenotypical characteristics. Lhx9GCE/GCE mice were all phenotypically female, smaller in size, viable, but infertile. The specificity and efficacy of the Lhx9-GCE mouse line was verified by crossing it to a Rosa26-tdTomato reporter mouse line, which reveals the Cre recombinase activities in retinal amacrine cells, developing limbs, testis, hippocampal neurons, thalamic neurons, and cerebellar neurons. Taken together, the Lhx9-GCE mouse line could serve as a beneficial tool for lineage tracing and gene manipulation experiments.
KW - Amacrine cells
KW - Cre recombinase
KW - LIM-homeodomain
KW - Lhx9
KW - Retina
KW - Transcription factor
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U2 - 10.1002/dvg.22805
DO - 10.1002/dvg.22805
M3 - Article
C2 - 25112520
AN - SCOPUS:84927574650
SN - 1526-954X
VL - 52
SP - 827
EP - 832
JO - Genesis
JF - Genesis
IS - 9
ER -