Generation and characterization of Lhx9-GFPCreERT2 knock-in mouse line

Revathi Balasubramanian, Andrew Bui, Xiaoling Xie, Min Deng, Lin Gan

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

LHX9 is a LIM-homeodomain transcription factor essential for the development of gonads, spinal cord interneurons, and thalamic neurons to name a few. We recently reported the expression of LHX9 in retinal amacrine cells during development. In this study, we generated an Lhx9-GFPCreERT2 (GCE) knock-in mouse line by knocking-in a GCE cassette at the Lhx9 locus, thus inactivating endogenous Lhx9. Lhx9GCE/+ mice were viable, fertile, and displayed no overt phenotypical characteristics. Lhx9GCE/GCE mice were all phenotypically female, smaller in size, viable, but infertile. The specificity and efficacy of the Lhx9-GCE mouse line was verified by crossing it to a Rosa26-tdTomato reporter mouse line, which reveals the Cre recombinase activities in retinal amacrine cells, developing limbs, testis, hippocampal neurons, thalamic neurons, and cerebellar neurons. Taken together, the Lhx9-GCE mouse line could serve as a beneficial tool for lineage tracing and gene manipulation experiments.

Original languageEnglish (US)
Pages (from-to)827-832
Number of pages6
JournalGenesis
Volume52
Issue number9
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

Keywords

  • Amacrine cells
  • Cre recombinase
  • LIM-homeodomain
  • Lhx9
  • Retina
  • Transcription factor

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

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