Generation of C57BL/6 knockout mice using C3H x BALB/c blastocysts

Gabriela Racholczyk, Rupali Suhag, Magdalena Mazurek, Suzanne M. Dederscheck, Pandelakis A. Koni

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The International Mouse Knockout Consortium aims to generate a knockout mouse for every single gene on a C57BL/6 background. Our ability to generate such mice is hampered by the poor economics of producing blastocysts to achieve germline transmission of C57BL/6 embryonic stem (ES) cells. We demonstrate superior utility of (C3H x BALB/c)F1 blastocysts compared with BALB/c blastocysts, with blastocyst numbers and germline transmission from subsequent chimeras at a rate 2- to 3-fold higher than that produced with BALB/c blastocysts.

Original languageEnglish (US)
Pages (from-to)413-416
Number of pages4
JournalBioTechniques
Volume44
Issue number3
DOIs
StatePublished - Mar 1 2008

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Generation of C57BL/6 knockout mice using C3H x BALB/c blastocysts'. Together they form a unique fingerprint.

  • Cite this

    Racholczyk, G., Suhag, R., Mazurek, M., Dederscheck, S. M., & Koni, P. A. (2008). Generation of C57BL/6 knockout mice using C3H x BALB/c blastocysts. BioTechniques, 44(3), 413-416. https://doi.org/10.2144/000112706