Generation of C57BL/6 knockout mice using C3H x BALB/c blastocysts

Gabriela Racholczyk, Rupali Suhag, Magdalena Mazurek, Suzanne M. Dederscheck, Pandelakis Koni

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The International Mouse Knockout Consortium aims to generate a knockout mouse for every single gene on a C57BL/6 background. Our ability to generate such mice is hampered by the poor economics of producing blastocysts to achieve germline transmission of C57BL/6 embryonic stem (ES) cells. We demonstrate superior utility of (C3H x BALB/c)F1 blastocysts compared with BALB/c blastocysts, with blastocyst numbers and germline transmission from subsequent chimeras at a rate 2- to 3-fold higher than that produced with BALB/c blastocysts.

Original languageEnglish (US)
Pages (from-to)413-416
Number of pages4
JournalBioTechniques
Volume44
Issue number3
DOIs
StatePublished - Mar 1 2008

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Blastocyst
Knockout Mice
Stem cells
Genes
Economics
Embryonic Stem Cells

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Racholczyk, G., Suhag, R., Mazurek, M., Dederscheck, S. M., & Koni, P. (2008). Generation of C57BL/6 knockout mice using C3H x BALB/c blastocysts. BioTechniques, 44(3), 413-416. https://doi.org/10.2144/000112706

Generation of C57BL/6 knockout mice using C3H x BALB/c blastocysts. / Racholczyk, Gabriela; Suhag, Rupali; Mazurek, Magdalena; Dederscheck, Suzanne M.; Koni, Pandelakis.

In: BioTechniques, Vol. 44, No. 3, 01.03.2008, p. 413-416.

Research output: Contribution to journalArticle

Racholczyk, G, Suhag, R, Mazurek, M, Dederscheck, SM & Koni, P 2008, 'Generation of C57BL/6 knockout mice using C3H x BALB/c blastocysts', BioTechniques, vol. 44, no. 3, pp. 413-416. https://doi.org/10.2144/000112706
Racholczyk G, Suhag R, Mazurek M, Dederscheck SM, Koni P. Generation of C57BL/6 knockout mice using C3H x BALB/c blastocysts. BioTechniques. 2008 Mar 1;44(3):413-416. https://doi.org/10.2144/000112706
Racholczyk, Gabriela ; Suhag, Rupali ; Mazurek, Magdalena ; Dederscheck, Suzanne M. ; Koni, Pandelakis. / Generation of C57BL/6 knockout mice using C3H x BALB/c blastocysts. In: BioTechniques. 2008 ; Vol. 44, No. 3. pp. 413-416.
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