Generation of C57BL/6 knockout mice using C3H x BALB/c blastocysts

Gabriela Racholczyk; Rupali Suhag; Magdalena Mazurek; Suzanne M. Dederscheck; Pandelakis A. Koni

doi:10.2144/000112706

Generation of C57BL/6 knockout mice using C3H x BALB/c blastocysts

Gabriela Racholczyk, Rupali Suhag, Magdalena Mazurek, Suzanne M. Dederscheck, Pandelakis A. Koni

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The International Mouse Knockout Consortium aims to generate a knockout mouse for every single gene on a C57BL/6 background. Our ability to generate such mice is hampered by the poor economics of producing blastocysts to achieve germline transmission of C57BL/6 embryonic stem (ES) cells. We demonstrate superior utility of (C3H x BALB/c)F₁ blastocysts compared with BALB/c blastocysts, with blastocyst numbers and germline transmission from subsequent chimeras at a rate 2- to 3-fold higher than that produced with BALB/c blastocysts.

Original language	English (US)
Pages (from-to)	413-416
Number of pages	4
Journal	BioTechniques
Volume	44
Issue number	3
DOIs	https://doi.org/10.2144/000112706
State	Published - Mar 2008
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
General Biochemistry, Genetics and Molecular Biology

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abstract = "The International Mouse Knockout Consortium aims to generate a knockout mouse for every single gene on a C57BL/6 background. Our ability to generate such mice is hampered by the poor economics of producing blastocysts to achieve germline transmission of C57BL/6 embryonic stem (ES) cells. We demonstrate superior utility of (C3H x BALB/c)F1 blastocysts compared with BALB/c blastocysts, with blastocyst numbers and germline transmission from subsequent chimeras at a rate 2- to 3-fold higher than that produced with BALB/c blastocysts.",

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AU - Suhag, Rupali

AU - Mazurek, Magdalena

AU - Dederscheck, Suzanne M.

AU - Koni, Pandelakis A.

PY - 2008/3

Y1 - 2008/3

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AB - The International Mouse Knockout Consortium aims to generate a knockout mouse for every single gene on a C57BL/6 background. Our ability to generate such mice is hampered by the poor economics of producing blastocysts to achieve germline transmission of C57BL/6 embryonic stem (ES) cells. We demonstrate superior utility of (C3H x BALB/c)F1 blastocysts compared with BALB/c blastocysts, with blastocyst numbers and germline transmission from subsequent chimeras at a rate 2- to 3-fold higher than that produced with BALB/c blastocysts.

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Generation of C57BL/6 knockout mice using C3H x BALB/c blastocysts

Abstract

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