Generation of skeletal, smooth and low molecular weight non-muscle tropomyosin isoforms from the chicken tropomyosin 1 gene

Suzanne Forry-Schaudies, Nita Jane Maihle, Stephen H. Hughes

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We have determined the organization of the chicken tropomyosin 1 gene by sequencing the cloned genomic DNA. The single-copy gene spans approximately 11,000 bases and includes 12 exons. Comparison of cDNA and genomic sequences demonstrates that three tissuespecific tropomyosins are encoded by the gene: a 284 amino acid skeletal muscle β-tropomyosin, a 284 amino acid smooth muscle tropomyosin, and a 248 amino acid nonmuscle (fibroblast) β-tropomyosin. Skeletal and smooth muscle transcripts use the same putative promoter and transcription initiation site. However, they are alternatively spliced to generate mRNAs that differ in the region giving rise to amino acids 188 to 213 and 258 through the poly(A) site. The fibroblast transcript uses a promoter, initiation site and first exon that is distinct from that used for both the smooth and the skeletal muscle transcripts. However, beyond the first exon the fibroblast transcript undergoes splicing and polyadenylation that is identical with the smooth muscle transcript.

Original languageEnglish (US)
Pages (from-to)321-330
Number of pages10
JournalJournal of Molecular Biology
Volume211
Issue number2
DOIs
StatePublished - Jan 20 1990
Externally publishedYes

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Tropomyosin
Chickens
Protein Isoforms
Molecular Weight
Smooth Muscle
Amino Acids
Exons
Skeletal Muscle
Fibroblasts
Genes
Polyadenylation
Poly A
Transcription Initiation Site
Complementary DNA
Messenger RNA
DNA

ASJC Scopus subject areas

  • Virology

Cite this

Generation of skeletal, smooth and low molecular weight non-muscle tropomyosin isoforms from the chicken tropomyosin 1 gene. / Forry-Schaudies, Suzanne; Maihle, Nita Jane; Hughes, Stephen H.

In: Journal of Molecular Biology, Vol. 211, No. 2, 20.01.1990, p. 321-330.

Research output: Contribution to journalArticle

Forry-Schaudies, Suzanne ; Maihle, Nita Jane ; Hughes, Stephen H. / Generation of skeletal, smooth and low molecular weight non-muscle tropomyosin isoforms from the chicken tropomyosin 1 gene. In: Journal of Molecular Biology. 1990 ; Vol. 211, No. 2. pp. 321-330.
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