TY - JOUR
T1 - Generation of T cell hybridomas from naturally occurring FoxP3+ regulatory T cells.
AU - Singh, Nagendra
AU - Pacholczyk, Rafal W
AU - Iwashima, Makio
AU - Ignatowicz, Leszek
PY - 2011
Y1 - 2011
N2 - Generation of regulatory T cells (or Treg) derived hybridomas offers a tool to study their antigen specificity. T cells hybridomas are produced by fusing TCR α-β-thymoma BW5147 with highly dividing T cell population. In vitro anergy of Tregs is an obstacle in generation of highly dividing Treg population for their fusion. In this chapter, we describe a simple and efficient method to generate large number of blasting Treg and their successful fusion with thymoma BW5147. The resultant hybridomas lose Treg-specific transcription factor FoxP3, respond to antigenic stimulation by producing IL-2, and thus allow the evaluation of antigen specific, Tregs-derived TCRs.
AB - Generation of regulatory T cells (or Treg) derived hybridomas offers a tool to study their antigen specificity. T cells hybridomas are produced by fusing TCR α-β-thymoma BW5147 with highly dividing T cell population. In vitro anergy of Tregs is an obstacle in generation of highly dividing Treg population for their fusion. In this chapter, we describe a simple and efficient method to generate large number of blasting Treg and their successful fusion with thymoma BW5147. The resultant hybridomas lose Treg-specific transcription factor FoxP3, respond to antigenic stimulation by producing IL-2, and thus allow the evaluation of antigen specific, Tregs-derived TCRs.
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U2 - 10.1007/978-1-61737-979-6_3
DO - 10.1007/978-1-61737-979-6_3
M3 - Article
C2 - 21287327
AN - SCOPUS:79955677665
SN - 1064-3745
VL - 707
SP - 39
EP - 44
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -