Generation of T cell hybridomas from naturally occurring FoxP3+ regulatory T cells.

Nagendra Singh, Rafal W Pacholczyk, Makio Iwashima, Leszek Ignatowicz

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Generation of regulatory T cells (or Treg) derived hybridomas offers a tool to study their antigen specificity. T cells hybridomas are produced by fusing TCR α-β-thymoma BW5147 with highly dividing T cell population. In vitro anergy of Tregs is an obstacle in generation of highly dividing Treg population for their fusion. In this chapter, we describe a simple and efficient method to generate large number of blasting Treg and their successful fusion with thymoma BW5147. The resultant hybridomas lose Treg-specific transcription factor FoxP3, respond to antigenic stimulation by producing IL-2, and thus allow the evaluation of antigen specific, Tregs-derived TCRs.

Original languageEnglish (US)
Pages (from-to)39-44
Number of pages6
JournalMethods in molecular biology (Clifton, N.J.)
Volume707
DOIs
StatePublished - May 11 2011

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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