Genetic analysis of cerebellar lipids in mice susceptible to audiogenic seizures

T. N. Seyfried, D. Bernard, F. Mayeda, L. Macala, Robert K Yu

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The major neutral and acidic cerebellar lipids were studied in audiogenic seizure (AGS)-resistant C57BL 6 (B6) and AGS-susceptible DBA 2J (D2) mice. These cerebellar lipids were also studied in the D2.B6-Iasb congenic mice and in the B6D2F1 hybrids that are mostly AGS resistant. Except for the Iasb gene, which inhibits AGS susceptibility, the D2.B6-Iasb congenic mice are genetically similar to the D2 mice. Because subtle abnormalities in the chemical structure of membranes may underlie epilepsy, we wanted to determine if AGS susceptibility was associated with abnormalities in the distribution of cerebellar lipids. A new method for the analysis of total brain lipids was used in this study. Slight, but significant, differences were found between the B6 and D2 strains for the concentrations (μg/100 mg wet weight) of sphingomyelin, phosphatidylcholine, and cerebrosides. However, these differences may not be associated with differences in AGS susceptibility since the concentrations of these lipids in the AGS-resistant D2.B6-Iasb and B6D2F1 mice were more similar to the D2 than the B6 concentrations. The expression of Iasb is not responsible for the lipid differences found between the B6 and D2 mice.

Original languageEnglish (US)
Pages (from-to)590-595
Number of pages6
JournalExperimental Neurology
Volume84
Issue number3
DOIs
StatePublished - Jan 1 1984
Externally publishedYes

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Seizures
Lipids
Inbred DBA Mouse
Congenic Mice
Cerebrosides
Sphingomyelins
Phosphatidylcholines
Epilepsy
Weights and Measures
Membranes
Brain
Genes

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

Cite this

Genetic analysis of cerebellar lipids in mice susceptible to audiogenic seizures. / Seyfried, T. N.; Bernard, D.; Mayeda, F.; Macala, L.; Yu, Robert K.

In: Experimental Neurology, Vol. 84, No. 3, 01.01.1984, p. 590-595.

Research output: Contribution to journalArticle

Seyfried, T. N. ; Bernard, D. ; Mayeda, F. ; Macala, L. ; Yu, Robert K. / Genetic analysis of cerebellar lipids in mice susceptible to audiogenic seizures. In: Experimental Neurology. 1984 ; Vol. 84, No. 3. pp. 590-595.
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