Genetic and molecular mechanisms of age regulation (homeostasis) of blood coagulation

Kotoku Kurachi, Kezhong Zhang, Afshin Ameri, Jeffery Huo, Hideko Atoda, Sumiko Kurachi

Research output: Contribution to journalReview article

2 Citations (Scopus)

Abstract

Blood coagulation plays a critical role not only in hemostasis but also in many physiological and pathological conditions. Epidemiological studies have shown that blood coagulation capacity in humans increases with age. Towards understanding the underlying mechanisms, the age regulation of factor IX, a key blood coagulation factor, was extensively studied. A series of human factor IX minigenes, consisting of various components of the human factor IX gene, were constructed and subjected to systematic analyses with HepG2 cells in culture and over the entire life span of transgenic mice. These studies identified critical gene structures that are essential for the unique age-dependent expression patterns of the human factor IX gene - one acting by stabilizing gene transcription and another increasing the amount of mRNA present, presumably by augmenting mRNA stability. These studies have set the stage for analyzing the overall age-based regulatory mechanisms of blood coagulation.

Original languageEnglish (US)
Pages (from-to)189-196
Number of pages8
JournalIUBMB Life
Volume49
Issue number3
DOIs
StatePublished - Jan 1 2000
Externally publishedYes

Fingerprint

Factor IX
Blood Coagulation
Coagulation
Molecular Biology
Homeostasis
Blood
Human engineering
Genes
Messenger RNA
Blood Coagulation Factors
Age Factors
Essential Genes
RNA Stability
Hep G2 Cells
Transcription
Hemostasis
Transgenic Mice
Epidemiologic Studies
Cell Culture Techniques

Keywords

  • Aging
  • Cardiovascular disease
  • Factor IX
  • Hemophilia
  • Homeostasis
  • Thrombosis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Clinical Biochemistry
  • Cell Biology

Cite this

Genetic and molecular mechanisms of age regulation (homeostasis) of blood coagulation. / Kurachi, Kotoku; Zhang, Kezhong; Ameri, Afshin; Huo, Jeffery; Atoda, Hideko; Kurachi, Sumiko.

In: IUBMB Life, Vol. 49, No. 3, 01.01.2000, p. 189-196.

Research output: Contribution to journalReview article

Kurachi, Kotoku ; Zhang, Kezhong ; Ameri, Afshin ; Huo, Jeffery ; Atoda, Hideko ; Kurachi, Sumiko. / Genetic and molecular mechanisms of age regulation (homeostasis) of blood coagulation. In: IUBMB Life. 2000 ; Vol. 49, No. 3. pp. 189-196.
@article{2689c53bc93a468fa5cb809b4d65ba12,
title = "Genetic and molecular mechanisms of age regulation (homeostasis) of blood coagulation",
abstract = "Blood coagulation plays a critical role not only in hemostasis but also in many physiological and pathological conditions. Epidemiological studies have shown that blood coagulation capacity in humans increases with age. Towards understanding the underlying mechanisms, the age regulation of factor IX, a key blood coagulation factor, was extensively studied. A series of human factor IX minigenes, consisting of various components of the human factor IX gene, were constructed and subjected to systematic analyses with HepG2 cells in culture and over the entire life span of transgenic mice. These studies identified critical gene structures that are essential for the unique age-dependent expression patterns of the human factor IX gene - one acting by stabilizing gene transcription and another increasing the amount of mRNA present, presumably by augmenting mRNA stability. These studies have set the stage for analyzing the overall age-based regulatory mechanisms of blood coagulation.",
keywords = "Aging, Cardiovascular disease, Factor IX, Hemophilia, Homeostasis, Thrombosis",
author = "Kotoku Kurachi and Kezhong Zhang and Afshin Ameri and Jeffery Huo and Hideko Atoda and Sumiko Kurachi",
year = "2000",
month = "1",
day = "1",
doi = "10.1080/152165400306197",
language = "English (US)",
volume = "49",
pages = "189--196",
journal = "IUBMB Life",
issn = "1521-6543",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Genetic and molecular mechanisms of age regulation (homeostasis) of blood coagulation

AU - Kurachi, Kotoku

AU - Zhang, Kezhong

AU - Ameri, Afshin

AU - Huo, Jeffery

AU - Atoda, Hideko

AU - Kurachi, Sumiko

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Blood coagulation plays a critical role not only in hemostasis but also in many physiological and pathological conditions. Epidemiological studies have shown that blood coagulation capacity in humans increases with age. Towards understanding the underlying mechanisms, the age regulation of factor IX, a key blood coagulation factor, was extensively studied. A series of human factor IX minigenes, consisting of various components of the human factor IX gene, were constructed and subjected to systematic analyses with HepG2 cells in culture and over the entire life span of transgenic mice. These studies identified critical gene structures that are essential for the unique age-dependent expression patterns of the human factor IX gene - one acting by stabilizing gene transcription and another increasing the amount of mRNA present, presumably by augmenting mRNA stability. These studies have set the stage for analyzing the overall age-based regulatory mechanisms of blood coagulation.

AB - Blood coagulation plays a critical role not only in hemostasis but also in many physiological and pathological conditions. Epidemiological studies have shown that blood coagulation capacity in humans increases with age. Towards understanding the underlying mechanisms, the age regulation of factor IX, a key blood coagulation factor, was extensively studied. A series of human factor IX minigenes, consisting of various components of the human factor IX gene, were constructed and subjected to systematic analyses with HepG2 cells in culture and over the entire life span of transgenic mice. These studies identified critical gene structures that are essential for the unique age-dependent expression patterns of the human factor IX gene - one acting by stabilizing gene transcription and another increasing the amount of mRNA present, presumably by augmenting mRNA stability. These studies have set the stage for analyzing the overall age-based regulatory mechanisms of blood coagulation.

KW - Aging

KW - Cardiovascular disease

KW - Factor IX

KW - Hemophilia

KW - Homeostasis

KW - Thrombosis

UR - http://www.scopus.com/inward/record.url?scp=0034121896&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034121896&partnerID=8YFLogxK

U2 - 10.1080/152165400306197

DO - 10.1080/152165400306197

M3 - Review article

C2 - 10868909

AN - SCOPUS:0034121896

VL - 49

SP - 189

EP - 196

JO - IUBMB Life

JF - IUBMB Life

SN - 1521-6543

IS - 3

ER -