Genetic and physical mapping of a type 1 susceptibility gene (IDDM12) to a 100-kb phagemid artificial chromosome clone containing D2S72-CTLA4- D2S105 on chromosome 2q33

Michele P. Marron, Adina Zeidler, Leslie J. Raffel, Sarah E. Eckenrode, James J. Yang, Diane I. Hopkins, Henri Jean Garchon, Chaim O. Jacob, Manuel Serrano-Rios, Maria T.Martinez Larrad, Yongsoo Park, Jean Francois Bach, Jerome I. Rotter, Mark C.K. Yang, Jin-Xiong She

Research output: Contribution to journalArticle

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Abstract

Polymorphic markers within the CTLA4 gene on chromosome 2q33 have been shown to be associated with type 1 diabetes. Therefore, a gene responsible for the disease (IDDM12) most likely lies within a region of <1-2 cM of CTLA4. To define more precisely the IDDM12 interval, we genotyped a multiethnic (U.S. Caucasian, Mexican-American, French, Spanish, Korean, and Chinese) collection of 178 simplex and 350 multiplex families for 10 polymorphic markers within a genomic interval of ~300 kb, which contains the candidate genes CTLA4 and CD28. The order of these markers (D2S346, CD28, GGAA19E07, D2S307, D2S72, CTLA4, D2S105, and GATA52A04) was determined by sequence tagged site content mapping of bacterial artificial chromosome (BAC) and yeast artificial chromosome (YAC) clones. The transmission disequilibrium test (TDT) analyses of our data revealed significant association/linkage with three markers within CTLA4 and two immediate flanking markers (D2S72 and D2S105) on each side of CTLA4 but not with more distant markers including the candidate gene CD28. Tsp analyses revealed significant association only with the three polymorphic markers within the CTLA4 gene. The markers linked and associated with type 1 diabetes are contained within a phagemid artificial chromosome clone of 100 kb, suggesting that the IDDM12 locus is either CTLA4 or an unknown gene in very close proximity.

Original languageEnglish (US)
Pages (from-to)492-499
Number of pages8
JournalDiabetes
Volume49
Issue number3
DOIs
StatePublished - Jan 1 2000
Externally publishedYes

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Artificial Chromosomes
Physical Chromosome Mapping
Clone Cells
Chromosomes
Genes
Type 1 Diabetes Mellitus
Sequence Tagged Sites
Yeast Artificial Chromosomes
Bacterial Artificial Chromosomes
Insulin-Dependent Diabetes Mellitus 12

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Genetic and physical mapping of a type 1 susceptibility gene (IDDM12) to a 100-kb phagemid artificial chromosome clone containing D2S72-CTLA4- D2S105 on chromosome 2q33. / Marron, Michele P.; Zeidler, Adina; Raffel, Leslie J.; Eckenrode, Sarah E.; Yang, James J.; Hopkins, Diane I.; Garchon, Henri Jean; Jacob, Chaim O.; Serrano-Rios, Manuel; Larrad, Maria T.Martinez; Park, Yongsoo; Bach, Jean Francois; Rotter, Jerome I.; Yang, Mark C.K.; She, Jin-Xiong.

In: Diabetes, Vol. 49, No. 3, 01.01.2000, p. 492-499.

Research output: Contribution to journalArticle

Marron, MP, Zeidler, A, Raffel, LJ, Eckenrode, SE, Yang, JJ, Hopkins, DI, Garchon, HJ, Jacob, CO, Serrano-Rios, M, Larrad, MTM, Park, Y, Bach, JF, Rotter, JI, Yang, MCK & She, J-X 2000, 'Genetic and physical mapping of a type 1 susceptibility gene (IDDM12) to a 100-kb phagemid artificial chromosome clone containing D2S72-CTLA4- D2S105 on chromosome 2q33', Diabetes, vol. 49, no. 3, pp. 492-499. https://doi.org/10.2337/diabetes.49.3.492
Marron, Michele P. ; Zeidler, Adina ; Raffel, Leslie J. ; Eckenrode, Sarah E. ; Yang, James J. ; Hopkins, Diane I. ; Garchon, Henri Jean ; Jacob, Chaim O. ; Serrano-Rios, Manuel ; Larrad, Maria T.Martinez ; Park, Yongsoo ; Bach, Jean Francois ; Rotter, Jerome I. ; Yang, Mark C.K. ; She, Jin-Xiong. / Genetic and physical mapping of a type 1 susceptibility gene (IDDM12) to a 100-kb phagemid artificial chromosome clone containing D2S72-CTLA4- D2S105 on chromosome 2q33. In: Diabetes. 2000 ; Vol. 49, No. 3. pp. 492-499.
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abstract = "Polymorphic markers within the CTLA4 gene on chromosome 2q33 have been shown to be associated with type 1 diabetes. Therefore, a gene responsible for the disease (IDDM12) most likely lies within a region of <1-2 cM of CTLA4. To define more precisely the IDDM12 interval, we genotyped a multiethnic (U.S. Caucasian, Mexican-American, French, Spanish, Korean, and Chinese) collection of 178 simplex and 350 multiplex families for 10 polymorphic markers within a genomic interval of ~300 kb, which contains the candidate genes CTLA4 and CD28. The order of these markers (D2S346, CD28, GGAA19E07, D2S307, D2S72, CTLA4, D2S105, and GATA52A04) was determined by sequence tagged site content mapping of bacterial artificial chromosome (BAC) and yeast artificial chromosome (YAC) clones. The transmission disequilibrium test (TDT) analyses of our data revealed significant association/linkage with three markers within CTLA4 and two immediate flanking markers (D2S72 and D2S105) on each side of CTLA4 but not with more distant markers including the candidate gene CD28. Tsp analyses revealed significant association only with the three polymorphic markers within the CTLA4 gene. The markers linked and associated with type 1 diabetes are contained within a phagemid artificial chromosome clone of 100 kb, suggesting that the IDDM12 locus is either CTLA4 or an unknown gene in very close proximity.",
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AU - Yang, James J.

AU - Hopkins, Diane I.

AU - Garchon, Henri Jean

AU - Jacob, Chaim O.

AU - Serrano-Rios, Manuel

AU - Larrad, Maria T.Martinez

AU - Park, Yongsoo

AU - Bach, Jean Francois

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