Genetic characterization of kanamycin resistance in Campylobacter coli

D. E. Taylor, W. Yan, L. K. Ng, E. K. Manavathu, P. Courvalin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The restriction map of plasmid pIP1433 from Campylobacter coli BM2509, isolated in France, was constructed and the location of the kanamycin (Km) and tetracycline (Tc) resistance markers determined using probes for aphA-3 and tetO genes, respectively. These DNA probes were used to study a second Km- and Tc-resistant strain C. coli UA696, isolated in Canada. In this strain, the Km resistance determinant was located in the chromosome. Like pIP1433, the Km resistance determinant in C. coliUA696 specified a 3'-aminoglycoside phosphotransferase of type III whereas the tetO gene was located on a non-transmissible plasmid.

Original languageEnglish (US)
Pages (from-to)665-676
Number of pages12
JournalAnnales de l'Institut Pasteur. Microbiologie
Volume139
Issue number6
DOIs
StatePublished - Nov 1 1988

Fingerprint

Campylobacter coli
Kanamycin Resistance
Plasmids
Kanamycin Kinase
Tetracycline Resistance
Kanamycin
DNA Probes
Tetracycline
Genes
France
Canada
Chromosomes

Keywords

  • 3'-Aminoglycoside phosphotransferase
  • Campylobacter coli
  • DNA probe
  • Kanamycin
  • Plasmid
  • Resistance
  • Tetracycline

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Genetic characterization of kanamycin resistance in Campylobacter coli. / Taylor, D. E.; Yan, W.; Ng, L. K.; Manavathu, E. K.; Courvalin, P.

In: Annales de l'Institut Pasteur. Microbiologie, Vol. 139, No. 6, 01.11.1988, p. 665-676.

Research output: Contribution to journalArticle

Taylor, D. E. ; Yan, W. ; Ng, L. K. ; Manavathu, E. K. ; Courvalin, P. / Genetic characterization of kanamycin resistance in Campylobacter coli. In: Annales de l'Institut Pasteur. Microbiologie. 1988 ; Vol. 139, No. 6. pp. 665-676.
@article{74aa273117cf4dc89528b78625e8f7fe,
title = "Genetic characterization of kanamycin resistance in Campylobacter coli",
abstract = "The restriction map of plasmid pIP1433 from Campylobacter coli BM2509, isolated in France, was constructed and the location of the kanamycin (Km) and tetracycline (Tc) resistance markers determined using probes for aphA-3 and tetO genes, respectively. These DNA probes were used to study a second Km- and Tc-resistant strain C. coli UA696, isolated in Canada. In this strain, the Km resistance determinant was located in the chromosome. Like pIP1433, the Km resistance determinant in C. coliUA696 specified a 3'-aminoglycoside phosphotransferase of type III whereas the tetO gene was located on a non-transmissible plasmid.",
keywords = "3'-Aminoglycoside phosphotransferase, Campylobacter coli, DNA probe, Kanamycin, Plasmid, Resistance, Tetracycline",
author = "Taylor, {D. E.} and W. Yan and Ng, {L. K.} and Manavathu, {E. K.} and P. Courvalin",
year = "1988",
month = "11",
day = "1",
doi = "10.1016/0769-2609(88)90071-3",
language = "English (US)",
volume = "139",
pages = "665--676",
journal = "Annales de l'Institut Pasteur Microbiology",
issn = "0769-2609",
publisher = "Elsevier BV",
number = "6",

}

TY - JOUR

T1 - Genetic characterization of kanamycin resistance in Campylobacter coli

AU - Taylor, D. E.

AU - Yan, W.

AU - Ng, L. K.

AU - Manavathu, E. K.

AU - Courvalin, P.

PY - 1988/11/1

Y1 - 1988/11/1

N2 - The restriction map of plasmid pIP1433 from Campylobacter coli BM2509, isolated in France, was constructed and the location of the kanamycin (Km) and tetracycline (Tc) resistance markers determined using probes for aphA-3 and tetO genes, respectively. These DNA probes were used to study a second Km- and Tc-resistant strain C. coli UA696, isolated in Canada. In this strain, the Km resistance determinant was located in the chromosome. Like pIP1433, the Km resistance determinant in C. coliUA696 specified a 3'-aminoglycoside phosphotransferase of type III whereas the tetO gene was located on a non-transmissible plasmid.

AB - The restriction map of plasmid pIP1433 from Campylobacter coli BM2509, isolated in France, was constructed and the location of the kanamycin (Km) and tetracycline (Tc) resistance markers determined using probes for aphA-3 and tetO genes, respectively. These DNA probes were used to study a second Km- and Tc-resistant strain C. coli UA696, isolated in Canada. In this strain, the Km resistance determinant was located in the chromosome. Like pIP1433, the Km resistance determinant in C. coliUA696 specified a 3'-aminoglycoside phosphotransferase of type III whereas the tetO gene was located on a non-transmissible plasmid.

KW - 3'-Aminoglycoside phosphotransferase

KW - Campylobacter coli

KW - DNA probe

KW - Kanamycin

KW - Plasmid

KW - Resistance

KW - Tetracycline

UR - http://www.scopus.com/inward/record.url?scp=0024242268&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024242268&partnerID=8YFLogxK

U2 - 10.1016/0769-2609(88)90071-3

DO - 10.1016/0769-2609(88)90071-3

M3 - Article

C2 - 2855308

AN - SCOPUS:0024242268

VL - 139

SP - 665

EP - 676

JO - Annales de l'Institut Pasteur Microbiology

JF - Annales de l'Institut Pasteur Microbiology

SN - 0769-2609

IS - 6

ER -