TY - JOUR
T1 - Genetic labeling reveals novel cellular targets of schizophrenia susceptibility gene
T2 - Distribution of gaba and non-gaba erbb4-positive cells in adult mouse brain
AU - Bean, Jonathan C.
AU - Lin, Thiri W.
AU - Sathyamurthy, Anupama
AU - Liu, Fang
AU - Yin, Dong Min
AU - Xiong, Wen Cheng
AU - Mei, Lin
N1 - Publisher Copyright:
© 2014 the authors.
PY - 2014/10/1
Y1 - 2014/10/1
N2 - Neuregulin 1 (NRG1) and its receptor ErbB4 are schizophrenia risk genes. NRG1-ErbB4 signaling plays a critical role in neural development and regulates neurotransmission and synaptic plasticity. Nevertheless, its cellular targets remain controversial. ErbB4 was thought to express in excitatory neurons, although recent studies disputed this view. Using mice that express a fluorescent protein under the promoter of the ErbB4 gene, we determined in what cells ErbB4 is expressed and their identity. ErbB4 was widely expressed in the mouse brain, being highest in amygdala and cortex. Almost all ErbB4-positive cells were GABAergic in cortex, hippocampus, basal ganglia, and most of amygdala in neonatal and adult mice, suggesting GABAergic transmission as a major target of NRG1-ErbB4 signaling in these regions. Non-GABAergic, ErbB4-positive cells were present in thalamus, hypothalamus, midbrain, and hindbrain. In particular, ErbB4 is expressedinserotoninergicneurons of raphenuclei but not innorepinephrinergicneurons of thelocus ceruleus. Inhypothalamus, ErbB4 is present in neurons that express oxytocin. Finally, ErbB4 is expressed in a group of cells in the subcortical areas that are positive for S100 calcium binding protein β. These results identify novel cellular targets of NRG1-ErbB4 signaling.
AB - Neuregulin 1 (NRG1) and its receptor ErbB4 are schizophrenia risk genes. NRG1-ErbB4 signaling plays a critical role in neural development and regulates neurotransmission and synaptic plasticity. Nevertheless, its cellular targets remain controversial. ErbB4 was thought to express in excitatory neurons, although recent studies disputed this view. Using mice that express a fluorescent protein under the promoter of the ErbB4 gene, we determined in what cells ErbB4 is expressed and their identity. ErbB4 was widely expressed in the mouse brain, being highest in amygdala and cortex. Almost all ErbB4-positive cells were GABAergic in cortex, hippocampus, basal ganglia, and most of amygdala in neonatal and adult mice, suggesting GABAergic transmission as a major target of NRG1-ErbB4 signaling in these regions. Non-GABAergic, ErbB4-positive cells were present in thalamus, hypothalamus, midbrain, and hindbrain. In particular, ErbB4 is expressedinserotoninergicneurons of raphenuclei but not innorepinephrinergicneurons of thelocus ceruleus. Inhypothalamus, ErbB4 is present in neurons that express oxytocin. Finally, ErbB4 is expressed in a group of cells in the subcortical areas that are positive for S100 calcium binding protein β. These results identify novel cellular targets of NRG1-ErbB4 signaling.
KW - ErbB4
KW - NRG1
KW - Neuregulin
KW - Oxytocin
KW - S100
KW - Serotonin
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U2 - 10.1523/JNEUROSCI.2021-14.2014
DO - 10.1523/JNEUROSCI.2021-14.2014
M3 - Article
C2 - 25274830
AN - SCOPUS:84907481694
SN - 0270-6474
VL - 34
SP - 13549
EP - 13566
JO - Journal of Neuroscience
JF - Journal of Neuroscience
IS - 40
ER -