Genetic relationship among four indian breeds of sheep using rapd-pcr

K. Ganeshkumar, Pushpendra Kumar, T. K. Bhattacharya, B. Bhushan, A. K. Patel, Vivek Choudhary, Arjava Sharma

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The study was' carried out in four Indian sheep breeds namely, Marwari, Mandya, Madras Red and Muzaffarnagari maintained at different organised herds of the country. A total of six oligonucleotide primers were subjected to perform random amplified polymorphic DNA (RAPD) fingerprinting to estimate genetic relationship (relatedness) among different breeds of sheep. All the six primers showed polymorphism in producing bands. The average number of bands in different breeds ranged from 6 to 15 with size of 0.3 to 1.9 kb. Out of six primers, OPM-2 and ILO-17 produced comparatively large number of bands in agarose gel. The intrabreed relatedness in terms of band sharing ranged from 0.66 to 1.00, the highest being in Marwari, whereas, lowest in Muzaffarnagari breed. The interbreed relatedness in terms of mean average percentage difference (MAPD) was highest (9.44±1.66) between Marwari and Muzaffarnagari breed while lowestvalue (4.90±1.54) was found between Mandya and Mujaffarnagari. It may be concluded that the Mandya breed is closest to Marwari while Marwari breed is too distant from Muzaffarnagari breed.

Original languageEnglish (US)
Pages (from-to)177-183
Number of pages7
JournalJournal of Applied Animal Research
Volume24
Issue number2
DOIs
StatePublished - Jan 1 2003

Fingerprint

sheep breeds
genetic relationships
Sheep
breeds
DNA Primers
DNA Fingerprinting
Sepharose
Gels
random amplified polymorphic DNA technique
DNA primers
DNA fingerprinting
agarose
herds
gels
genetic polymorphism
India

Keywords

  • BSF
  • Genetic relationship
  • MAPD
  • RAPD
  • Sheep

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Ganeshkumar, K., Kumar, P., Bhattacharya, T. K., Bhushan, B., Patel, A. K., Choudhary, V., & Sharma, A. (2003). Genetic relationship among four indian breeds of sheep using rapd-pcr. Journal of Applied Animal Research, 24(2), 177-183. https://doi.org/10.1080/09712119.2003.9706454

Genetic relationship among four indian breeds of sheep using rapd-pcr. / Ganeshkumar, K.; Kumar, Pushpendra; Bhattacharya, T. K.; Bhushan, B.; Patel, A. K.; Choudhary, Vivek; Sharma, Arjava.

In: Journal of Applied Animal Research, Vol. 24, No. 2, 01.01.2003, p. 177-183.

Research output: Contribution to journalArticle

Ganeshkumar, K, Kumar, P, Bhattacharya, TK, Bhushan, B, Patel, AK, Choudhary, V & Sharma, A 2003, 'Genetic relationship among four indian breeds of sheep using rapd-pcr', Journal of Applied Animal Research, vol. 24, no. 2, pp. 177-183. https://doi.org/10.1080/09712119.2003.9706454
Ganeshkumar, K. ; Kumar, Pushpendra ; Bhattacharya, T. K. ; Bhushan, B. ; Patel, A. K. ; Choudhary, Vivek ; Sharma, Arjava. / Genetic relationship among four indian breeds of sheep using rapd-pcr. In: Journal of Applied Animal Research. 2003 ; Vol. 24, No. 2. pp. 177-183.
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