Genetic substitution of Cdk1 by Cdk2 leads to embryonic lethality and loss of meiotic function of Cdk2

Satyanarayana Ande, Cyril Berthet, Javier Lopez-Molina, Vincenzo Coppola, Lino Tessarollo, Philipp Kaldis

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

It was believed that Cdk2-cyclin E complexes are essential to drive cells through the G1-S phase transition. However, it was discovered recently that the mitotic kinase Cdk1 (Cdc2a) compensates for the loss of Cdk2. In the present study, we tested whether Cdk2 can compensate for the loss of Cdk1. We generated a knockin mouse in which the Cdk2 cDNA was knocked into the Cdk1 locus (Cdk1Cdk2KI). Substitution of both copies of Cdk1 by Cdk2 led to early embryonic lethality, even though Cdk2 was expressed from the Cdk1 locus. In addition, we generated Cdk2-/- Cdk1+/Cdk2KI mice in which one copy of Cdk2 and one copy of Cdk1 were expressed from the Cdk1 locus and the Cdk2 gene was deleted from the endogenous Cdk2 locus. We found that both male and female Cdk2-/- Cdk1+/Cdk2KI mice were sterile, similar to Cdk2-/- mice, even though they expressed the Cdk2 protein from the Cdk1 locus in testes. The translocational and cell cycle properties of knockin Cdk2 in Cdk2-/- Cdk1+/Cdk2KI cells were comparable to those of endogenous Cdk2, but we detected premature transcriptional activation of Cdk1 during liver regeneration in the absence of Cdk2. This study provides evidence of the molecular differences between Cdk2 and Cdk1 and highlights that the timing of transcriptional activation and the genetic locus play important roles in determining the function of Cdk proteins in vivo.

Original languageEnglish (US)
Pages (from-to)3389-3400
Number of pages12
JournalDevelopment
Volume135
Issue number20
DOIs
StatePublished - Dec 1 2008
Externally publishedYes

Fingerprint

Transcriptional Activation
CDC2 Protein Kinase
Cyclin E
Liver Regeneration
Genetic Loci
Cyclin-Dependent Kinases
Phase Transition
G1 Phase
S Phase
Testis
Cell Cycle
Complementary DNA
Genes
Proteins

Keywords

  • Cell cyde regulation
  • Cyclin
  • Cydin-dependent kinase (Cdk)
  • Meiosis
  • Mouse genetics

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology

Cite this

Ande, S., Berthet, C., Lopez-Molina, J., Coppola, V., Tessarollo, L., & Kaldis, P. (2008). Genetic substitution of Cdk1 by Cdk2 leads to embryonic lethality and loss of meiotic function of Cdk2. Development, 135(20), 3389-3400. https://doi.org/10.1242/dev.024919

Genetic substitution of Cdk1 by Cdk2 leads to embryonic lethality and loss of meiotic function of Cdk2. / Ande, Satyanarayana; Berthet, Cyril; Lopez-Molina, Javier; Coppola, Vincenzo; Tessarollo, Lino; Kaldis, Philipp.

In: Development, Vol. 135, No. 20, 01.12.2008, p. 3389-3400.

Research output: Contribution to journalArticle

Ande, S, Berthet, C, Lopez-Molina, J, Coppola, V, Tessarollo, L & Kaldis, P 2008, 'Genetic substitution of Cdk1 by Cdk2 leads to embryonic lethality and loss of meiotic function of Cdk2', Development, vol. 135, no. 20, pp. 3389-3400. https://doi.org/10.1242/dev.024919
Ande, Satyanarayana ; Berthet, Cyril ; Lopez-Molina, Javier ; Coppola, Vincenzo ; Tessarollo, Lino ; Kaldis, Philipp. / Genetic substitution of Cdk1 by Cdk2 leads to embryonic lethality and loss of meiotic function of Cdk2. In: Development. 2008 ; Vol. 135, No. 20. pp. 3389-3400.
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