Genistein decreases food intake, body weight, and fat pad weight and causes adipose tissue apoptosis in ovariectomized female mice

Hye Kyeong Kim, Cassandra Nelson-Dooley, Mary Anne Della-Fera, Jeong Yeh Yang, Wei Zhang, Jiuhua Duan, Diane L. Hartzell, Mark W Hamrick, Clifton A. Baile

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

Genistein, an isoflavone in soybean products, has estrogenic activity and is used as a natural substitute for estrogen replacement therapy in postmenopausal women. Genistein was also shown to decrease fat pad weight in female mice. The primary objective of this study was to determine the effect of genistein on adipose tissue apoptosis in vitro and in vivo. 3T3-L1 preadipocytes and mature adipocytes were treated with 0, 1, 10, 100, and 400 μmol/L genistein and then assayed for apoptosis, whereas only mature adipocytes were assayed for viability. Mature adipocytes treated with genistein demonstrated a dose-related increase in apoptosis. Ovariectomized female mice (9 mo old) were given 0, 150, or 1500 mg/kg genistein in the semipurified phytoestrogen-free casein-based diet for 3 wk (n = 10). After mice were killed, body composition was determined by dual-energy X-ray absorptiometry analysis, and parametrial (PM), inguinal (ING), and retroperitoneal (RP) fat pads were weighed and assayed for apoptosis (% DNA fragmentation). Genistein (1500 mg/kg) reduced food intake (FI) by 14% (P < 0.01) and body weight (BW) by 9% (P < 0.01). Body composition was not significantly affected, but PM and ING weights were decreased 22% (P < 0.05) and 19% (P < 0.07), respectively, by 1500 mg/kg genistein. Apoptosis in ING fat was increased 290% (P < 0.05) by 1500 mg/kg genistein. These findings show that oral genistein treatment can reduce BW, mobilize body fat, and induce apoptosis of adipose tissue in ovariectomized female mice. Thus, genistein may be useful in treating or preventing increased adiposity after menopause.

Original languageEnglish (US)
Pages (from-to)409-414
Number of pages6
JournalJournal of Nutrition
Volume136
Issue number2
StatePublished - Feb 1 2006

Fingerprint

Genistein
Adipose Tissue
Eating
Body Weight
Apoptosis
Weights and Measures
Groin
Adipocytes
Body Composition
Phytoestrogens
Estrogen Replacement Therapy
Isoflavones
Intra-Abdominal Fat
Photon Absorptiometry
Adiposity
DNA Fragmentation
Menopause
Caseins
Soybeans
Fats

Keywords

  • 3T3-L1 adipocytes
  • Adipose tissue
  • Apoptosis
  • Isoflavone
  • Weight loss

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Kim, H. K., Nelson-Dooley, C., Della-Fera, M. A., Yang, J. Y., Zhang, W., Duan, J., ... Baile, C. A. (2006). Genistein decreases food intake, body weight, and fat pad weight and causes adipose tissue apoptosis in ovariectomized female mice. Journal of Nutrition, 136(2), 409-414.

Genistein decreases food intake, body weight, and fat pad weight and causes adipose tissue apoptosis in ovariectomized female mice. / Kim, Hye Kyeong; Nelson-Dooley, Cassandra; Della-Fera, Mary Anne; Yang, Jeong Yeh; Zhang, Wei; Duan, Jiuhua; Hartzell, Diane L.; Hamrick, Mark W; Baile, Clifton A.

In: Journal of Nutrition, Vol. 136, No. 2, 01.02.2006, p. 409-414.

Research output: Contribution to journalArticle

Kim, HK, Nelson-Dooley, C, Della-Fera, MA, Yang, JY, Zhang, W, Duan, J, Hartzell, DL, Hamrick, MW & Baile, CA 2006, 'Genistein decreases food intake, body weight, and fat pad weight and causes adipose tissue apoptosis in ovariectomized female mice', Journal of Nutrition, vol. 136, no. 2, pp. 409-414.
Kim HK, Nelson-Dooley C, Della-Fera MA, Yang JY, Zhang W, Duan J et al. Genistein decreases food intake, body weight, and fat pad weight and causes adipose tissue apoptosis in ovariectomized female mice. Journal of Nutrition. 2006 Feb 1;136(2):409-414.
Kim, Hye Kyeong ; Nelson-Dooley, Cassandra ; Della-Fera, Mary Anne ; Yang, Jeong Yeh ; Zhang, Wei ; Duan, Jiuhua ; Hartzell, Diane L. ; Hamrick, Mark W ; Baile, Clifton A. / Genistein decreases food intake, body weight, and fat pad weight and causes adipose tissue apoptosis in ovariectomized female mice. In: Journal of Nutrition. 2006 ; Vol. 136, No. 2. pp. 409-414.
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abstract = "Genistein, an isoflavone in soybean products, has estrogenic activity and is used as a natural substitute for estrogen replacement therapy in postmenopausal women. Genistein was also shown to decrease fat pad weight in female mice. The primary objective of this study was to determine the effect of genistein on adipose tissue apoptosis in vitro and in vivo. 3T3-L1 preadipocytes and mature adipocytes were treated with 0, 1, 10, 100, and 400 μmol/L genistein and then assayed for apoptosis, whereas only mature adipocytes were assayed for viability. Mature adipocytes treated with genistein demonstrated a dose-related increase in apoptosis. Ovariectomized female mice (9 mo old) were given 0, 150, or 1500 mg/kg genistein in the semipurified phytoestrogen-free casein-based diet for 3 wk (n = 10). After mice were killed, body composition was determined by dual-energy X-ray absorptiometry analysis, and parametrial (PM), inguinal (ING), and retroperitoneal (RP) fat pads were weighed and assayed for apoptosis ({\%} DNA fragmentation). Genistein (1500 mg/kg) reduced food intake (FI) by 14{\%} (P < 0.01) and body weight (BW) by 9{\%} (P < 0.01). Body composition was not significantly affected, but PM and ING weights were decreased 22{\%} (P < 0.05) and 19{\%} (P < 0.07), respectively, by 1500 mg/kg genistein. Apoptosis in ING fat was increased 290{\%} (P < 0.05) by 1500 mg/kg genistein. These findings show that oral genistein treatment can reduce BW, mobilize body fat, and induce apoptosis of adipose tissue in ovariectomized female mice. Thus, genistein may be useful in treating or preventing increased adiposity after menopause.",
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