Genistein suppresses adhesion-induced protein tyrosine phosphorylation and invasion of B16-BL6 melanoma cells

Chunhong Yan, Rui Han

Research output: Contribution to journalArticle

26 Scopus citations


Protein tyrosine phosphorylation occurs as one of the earlier events in cancer cell-extracellular matrix (ECM) interaction. With immunoblot analysis and immunofluorescence microscopy, genistein was found to suppress the tyrosine phosphorylation of proteins located at the cell periphery, including a 125 kDa protein, when B16-BL6 melanoma cells attached to and interacted with ECM. When accompanied by the suppression of adhesion-induced protein tyrosine phosphorylation, the invasive potential of B16-BL6 cells through reconstituted basement membrane was decreased significantly. However, neither adhesive capability nor cell growth was significantly affected by genistein. Therefore, the interruption of cancer cell-ECM interaction by suppression of protein tyrosine phosphorylation may contribute to invasion prevention of genistein.

Original languageEnglish (US)
Pages (from-to)117-124
Number of pages8
JournalCancer Letters
Issue number1
Publication statusPublished - Jul 3 1998
Externally publishedYes



  • Cell-extracellular matrix interaction
  • Genistein
  • Invasion
  • Protein tyrosine phosphorylation

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this