Genomic expression analysis by single-cell mRNA differential display of quiescent CD8 T cells from tumour-infiltrating lymphocytes obtained from in vivo liver tumours

Wei Zhang, Jianqing Ding, Yan Qu, Hongliang Hu, Meihua Lin, Amit Datta, Alan Larson, George E. Liu, Biaoru Li

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

We performed a genomic study combining single-cell mRNA differential display and RNA subtractive hybridization to elucidate CD8 T-cell quiescence/ignorance. By comparing actively maintained quiescent CD8 T cells from liver tumour tumour-infiltrating lymphocytes (TILs) with quiescent T cells at the single-cell level, we identified differentially expressed candidate genes by high-throughput screening and comparative analysis of expressed sequence tags (ESTs). While genes for the T-cell receptor, tumour necrosis factor (TNF) receptor, TNF-related apoptpsis inducing ligand (TRAIL) and perforin were down-regulated, key genes such as Tob, transforming growth factor (TGF)-β, lung Krüpple-like factor (LKLF), Sno-A, Ski, Myc, Ets-2 repressor factor (ERF) and RE1-silencing transcription factor (REST/NRSF) complex were highly expressed in the quiescent TIL CD8 cells. Real-time polymerase chain reaction (PCR) further confirmed these results. A regulation model is proposed for actively maintained quiescence in CD8 T cells, including three components: up-regulation of the TGF-β pathway, a shift in the MYC web and inhibition of the cell cycle.

Original languageEnglish (US)
Pages (from-to)83-90
Number of pages8
JournalImmunology
Volume127
Issue number1
DOIs
Publication statusPublished - May 1 2009

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Keywords

  • CD8
  • Gene expression profiling
  • Quiescence
  • T cells
  • Tumour-infiltrating lymphocytes (TILs)

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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