Genomic organization, 5′-flanking region, and chromosomal localization of the human RGS3 gene

Tapan Kumar Chatterjee, Alex Eapen, Adam B. Kanis, Rory A. Fisher

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

RGS3 is the largest member of a recently discovered family of proteins (RGS proteins) that appear to function as negative regulators of heterotrimeric G-protein signaling. Seventeen mammalian RGS proteins have been identified by cloning or by comparison to expressed sequence tags, and several of these proteins have been shown recently to function as GTPase-activating proteins for G-protein α subunits. Despite the intense interest in RGS proteins as physiological regulators of G-protein signaling, there is little understanding of the structure and regulation of mammalian RGS genes. Using long-distance PCR, we amplified and characterized the entire coding and 5′-untranslated region of the human RGS3 gene. The coding region of the human RGS3 gene spans 14.7 kb and contains six exons, and the 5′-untranslated region spans 3.2 kb and contains two exons. Mapping of the exons revealed that the RGS domain, conserved among all RGS proteins, was encoded by three exons, while the unique amino-terminal domain of RGS3 was encoded by a single exon. Comparison of the location of the intron-exon boundaries of the human RGS3 gene to that of the human RGS2 gene, the only mammalian RGS gene described previously, revealed a remarkable similarity, providing the first conceptual support for a common ancestral mammalian RGS gene. 5′-RACE analysis was used to map the transcription start site 517 bp upstream of the translation start site, and anchored PCR was performed to amplify 1.0 kb of genomic DNA upstream of the transcription start site. Analysis of the 5′-flanking region revealed the presence of many potential regulatory elements, the presence of an initiator (Inr) element overlapping the transcription start site, and the absence of a TATA or a CCAAT box at the usual positions. By radiation hybrid mapping, the RGS3 gene was assigned to human chromosome 9q31-q33. This study is the first to elucidate the structure, chromosomal location, and regulatory sequences of the RGS3 gene, and it establishes the genetic basis for RGS3 gene research in humans.

Original languageEnglish (US)
Article numberGE974929
Pages (from-to)429-433
Number of pages5
JournalGenomics
Volume45
Issue number2
DOIs
StatePublished - Jan 1 1997

Fingerprint

5' Flanking Region
RGS Proteins
Exons
Genes
Transcription Initiation Site
GTP-Binding Protein Regulators
5' Untranslated Regions
Radiation Hybrid Mapping
GTPase-Activating Proteins
Heterotrimeric GTP-Binding Proteins
Polymerase Chain Reaction
Expressed Sequence Tags
Protein Subunits
Human Chromosomes
GTP-Binding Proteins
Introns
Organism Cloning
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Chatterjee, T. K., Eapen, A., Kanis, A. B., & Fisher, R. A. (1997). Genomic organization, 5′-flanking region, and chromosomal localization of the human RGS3 gene. Genomics, 45(2), 429-433. [GE974929]. https://doi.org/10.1006/geno.1997.4929

Genomic organization, 5′-flanking region, and chromosomal localization of the human RGS3 gene. / Chatterjee, Tapan Kumar; Eapen, Alex; Kanis, Adam B.; Fisher, Rory A.

In: Genomics, Vol. 45, No. 2, GE974929, 01.01.1997, p. 429-433.

Research output: Contribution to journalArticle

Chatterjee, TK, Eapen, A, Kanis, AB & Fisher, RA 1997, 'Genomic organization, 5′-flanking region, and chromosomal localization of the human RGS3 gene', Genomics, vol. 45, no. 2, GE974929, pp. 429-433. https://doi.org/10.1006/geno.1997.4929
Chatterjee, Tapan Kumar ; Eapen, Alex ; Kanis, Adam B. ; Fisher, Rory A. / Genomic organization, 5′-flanking region, and chromosomal localization of the human RGS3 gene. In: Genomics. 1997 ; Vol. 45, No. 2. pp. 429-433.
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