Genomic screen for expression pattern in adult Drosophila brains In vivo by enhancer trap method and establishment of expression database

Xiao Jun Liu, Xiao Jing Yuan, Kan Sun, Yi Chun Shuai, Qing Xuan Song, Lei Wang, Li Sha Shao, Xin Yu Zhao, Yi Sheng Lü, Yu Bing Lü, Lian Zhang Wang, Yi Zhong

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

"Enhancer trap" is an important technique to build and explore Drosophila brain gene expression pattern and its database. Large genome-wide screen for specific expression pattern in adult Drosophila brains by enhancer trap method provides fly tools for further understanding neural basis of learning and memory. Enhancer trap method was utilized to generate Drosophila P[Gal4] mutant library. Each mutant in library was crossed with GFP reporter flies (UAS-eGFP); heads of the adult off-springs were dissected to obtain the whole brain which were examined under the epifluorescence microscope and expression pattern of each mutant were further analyzed. JavaScript was utilized to establish an expression pattern library. A total of 2 677 mutant lines of Drosophila were obtained by P-element induced mutagenesis and subjected to expression pattern analysis. Most of mutated genes express in adult fly. 368 mutated genes specific express in the mushroom body, which was reported to involve in Drosophila olfactory learning and memory, and some others specific express in other neuron circuitries. Drosophila mutant libraries and genome-wide expression pattern database was built by enhancer trap method, which as a tool will be utilized to understand the function of genes and particular neural circuitries in Drosophila in the future.

Original languageEnglish (US)
Pages (from-to)645-649
Number of pages5
JournalProgress in Biochemistry and Biophysics
Volume35
Issue number6
StatePublished - Jun 1 2008

Keywords

  • Enhancer trap method
  • Expression pattern
  • Mushroom body
  • Mutant libraries
  • Reporter transgenic flies

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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    Liu, X. J., Yuan, X. J., Sun, K., Shuai, Y. C., Song, Q. X., Wang, L., Shao, L. S., Zhao, X. Y., Lü, Y. S., Lü, Y. B., Wang, L. Z., & Zhong, Y. (2008). Genomic screen for expression pattern in adult Drosophila brains In vivo by enhancer trap method and establishment of expression database. Progress in Biochemistry and Biophysics, 35(6), 645-649.