Germ cell transplantation in infertility mouse

Xiayang Xie, Yong Teng, Lianghong Ma, Lijun Liu, Xiang Wang, Yingching Song, Ping Xu

Research output: Contribution to journalArticle

Abstract

This work investigated the spermatogenesis in an infertility BALB/c-nu mouse model by reinfusing germline stem cells into seminiferous tubules. Donor germ cells were isolated from male FVB/NJ-GFP transgenic mice. Seminiferous tubule microinjection was applied to achieve intratubular germ cell transfer. The germ cells were injected into exposed testes of the infertility mice. We used green fluorescence and DNA analysis of donor cells from GFP transgenic mice as genetic marker. The natural mating and Southern blot methods were applied to analyze the effect of sperm cell transplantation and the sperm function after seminiferous tubule microinjection. The spermatogenesis was morphologically observed from the seminiferous tubules in 41/60 (68.33%) of the injected recipient mice using allogeneic donor cells. In the colonized testes, matured spermatozoa were seen in the lumen of the seminiferous tubules. In this research, BALB/c-nu infertility mouse model, the recipient animal, was used to avoid immunological rejection of donor cells, and germ cell transplantation was applied to overcome infertility caused by busulfan treatment. These results demonstrate that this technique of germ cell transplantation is of great use. Germ cell transplantation could be potentially valuable to oncological patients.

Original languageEnglish (US)
Pages (from-to)70-75
Number of pages6
JournalChinese Science Bulletin
Volume53
Issue number1
DOIs
StatePublished - Jan 1 2008

Keywords

  • GFP
  • Germ cell
  • Infertility
  • Microinjection
  • Transplantation

ASJC Scopus subject areas

  • General

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  • Cite this

    Xie, X., Teng, Y., Ma, L., Liu, L., Wang, X., Song, Y., & Xu, P. (2008). Germ cell transplantation in infertility mouse. Chinese Science Bulletin, 53(1), 70-75. https://doi.org/10.1007/s11434-008-0027-9