Ghrelin Inhibits Proinflammatory Responses and Nuclear Factor-κB Activation in Human Endothelial Cells

Wei Gen Li, Dan Gavrila, Xuebo Liu, Lixing Wang, Skuli Gunnlaugsson, Lynn L. Stoll, Michael L. McCormick, Curt D. Sigmund, Chaosu Tang, Neal Lee Weintraub

Research output: Contribution to journalArticle

407 Citations (Scopus)

Abstract

Background-Ghrelin is a novel growth hormone-releasing peptide that has been shown to improve cachexia in heart failure and cancer and to ameliorate the hemodynamic and metabolic disturbances in septic shock. Because cytokine-induced inflammation is critical in these pathological states and because the growth hormone secretagogue receptor has been identified in blood vessels, we examined whether ghrelin inhibits proinflammatory responses in human endothelial cells in vitro and after administration of endotoxin to rats in vivo. Methods and Results-Human umbilical vein endothelial cells (HUVECs) were treated with or without tumor necrosis factor-α (TNF-α), and induction of proinflammitory cytokines and mononuclear cell adhesion were determined. Ghrelin (0.1 to 1000 ng/mL) inhibited both basal and TNF-α-induced cytokine release and mononuclear cell binding. Intravenous administration of ghrelin also inhibited endotoxin-induced proinflammatory cytokine production in rats in vivo. Ghrelin inhibited H2O 2-induced cytokine release in HUVECs, suggesting that the peptide blocks redox-mediated cellular signaling. Moreover, ghrelin inhibited basal and TNF-α-induced activation of nuclear factor-κB. Des-acyl ghrelin had no effect on TNF-α-induced cytokine production in HUVECs, suggesting that the antiinflammatory effects of ghrelin require interaction with endothelial growth hormone secretagogue receptors. Conclusions-Ghrelin inhibits proinflammatory cytokine production, mononuclear cell binding, and nuclear factor-κB activation in human endothelial cells in vitro and endotoxin-induced cytokine production in vivo. These novel antiinflammatory actions of ghrelin suggest that the peptide could play a modulatory role in atherosclerosis, especially in obese patients, in whom ghrelin levels are reduced.

Original languageEnglish (US)
Pages (from-to)2221-2226
Number of pages6
JournalCirculation
Volume109
Issue number18
DOIs
StatePublished - May 11 2004
Externally publishedYes

Fingerprint

Ghrelin
Endothelial Cells
Cytokines
Human Umbilical Vein Endothelial Cells
Tumor Necrosis Factor-alpha
Endotoxins
Ghrelin Receptor
Anti-Inflammatory Agents
Peptides
Cachexia
Heart Neoplasms
Septic Shock
Cell Adhesion
Intravenous Administration
Oxidation-Reduction
Blood Vessels
Atherosclerosis
Heart Failure
Hemodynamics
Inflammation

Keywords

  • Endotoxins
  • Hormones, peptide
  • Inflammation
  • Monocytes
  • Nuclear factor-κB

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Ghrelin Inhibits Proinflammatory Responses and Nuclear Factor-κB Activation in Human Endothelial Cells. / Li, Wei Gen; Gavrila, Dan; Liu, Xuebo; Wang, Lixing; Gunnlaugsson, Skuli; Stoll, Lynn L.; McCormick, Michael L.; Sigmund, Curt D.; Tang, Chaosu; Weintraub, Neal Lee.

In: Circulation, Vol. 109, No. 18, 11.05.2004, p. 2221-2226.

Research output: Contribution to journalArticle

Li, WG, Gavrila, D, Liu, X, Wang, L, Gunnlaugsson, S, Stoll, LL, McCormick, ML, Sigmund, CD, Tang, C & Weintraub, NL 2004, 'Ghrelin Inhibits Proinflammatory Responses and Nuclear Factor-κB Activation in Human Endothelial Cells', Circulation, vol. 109, no. 18, pp. 2221-2226. https://doi.org/10.1161/01.CIR.0000127956.43874.F2
Li, Wei Gen ; Gavrila, Dan ; Liu, Xuebo ; Wang, Lixing ; Gunnlaugsson, Skuli ; Stoll, Lynn L. ; McCormick, Michael L. ; Sigmund, Curt D. ; Tang, Chaosu ; Weintraub, Neal Lee. / Ghrelin Inhibits Proinflammatory Responses and Nuclear Factor-κB Activation in Human Endothelial Cells. In: Circulation. 2004 ; Vol. 109, No. 18. pp. 2221-2226.
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abstract = "Background-Ghrelin is a novel growth hormone-releasing peptide that has been shown to improve cachexia in heart failure and cancer and to ameliorate the hemodynamic and metabolic disturbances in septic shock. Because cytokine-induced inflammation is critical in these pathological states and because the growth hormone secretagogue receptor has been identified in blood vessels, we examined whether ghrelin inhibits proinflammatory responses in human endothelial cells in vitro and after administration of endotoxin to rats in vivo. Methods and Results-Human umbilical vein endothelial cells (HUVECs) were treated with or without tumor necrosis factor-α (TNF-α), and induction of proinflammitory cytokines and mononuclear cell adhesion were determined. Ghrelin (0.1 to 1000 ng/mL) inhibited both basal and TNF-α-induced cytokine release and mononuclear cell binding. Intravenous administration of ghrelin also inhibited endotoxin-induced proinflammatory cytokine production in rats in vivo. Ghrelin inhibited H2O 2-induced cytokine release in HUVECs, suggesting that the peptide blocks redox-mediated cellular signaling. Moreover, ghrelin inhibited basal and TNF-α-induced activation of nuclear factor-κB. Des-acyl ghrelin had no effect on TNF-α-induced cytokine production in HUVECs, suggesting that the antiinflammatory effects of ghrelin require interaction with endothelial growth hormone secretagogue receptors. Conclusions-Ghrelin inhibits proinflammatory cytokine production, mononuclear cell binding, and nuclear factor-κB activation in human endothelial cells in vitro and endotoxin-induced cytokine production in vivo. These novel antiinflammatory actions of ghrelin suggest that the peptide could play a modulatory role in atherosclerosis, especially in obese patients, in whom ghrelin levels are reduced.",
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AU - Liu, Xuebo

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AU - Gunnlaugsson, Skuli

AU - Stoll, Lynn L.

AU - McCormick, Michael L.

AU - Sigmund, Curt D.

AU - Tang, Chaosu

AU - Weintraub, Neal Lee

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