Gliosarcoma cell death after radiosurgery in a rat model

Tom G. Psarros, B. Mickey, J. Gilio, J. Drees, K. Gall, D. Carlson, Cole A. Giller, M. S. Willis

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Therapeutic radiation and subsequent detection of tumor cell death has been performed mainly in vitro systems, making it difficult to accurately characterize the mechanisms of tumor cell death after radiosurgery. To better characterize what occurs to glioma cells after radiation therapy, we developed a rat model using the 9L gliosarcoma cell line implanted reproducibly to the caudate nucleus in rats. After 1 Gy radiation, 9L tumors in vivo induced mainly necrosis (determined by trypan blue exclusion) of 10-74% at 6-72 hours post-radiation. This is in contrast to a previous in vitro study which demonstrated that 18 Gy of radiation induces considerably less cell death as determined by trypan blue exclusion (approximately 20-25% at 6-72 hours post-radiation). However, significant amounts of apoptosis were detected as early as 6 hours after radiation. Apoptosis determination was by annexin V (marker of early apoptosis) and propidium iodide (marker of membrane stability) staining followed by flow cytometry detection. When caspase 3 and caspase 8 enzymatic activities (mediators of apoptosis) were measured from freshly explanted tumor cells, peak activity was found 6 hours after 1 Gy radiation (p < 0.01). Taken together, these data indicate the presence of apoptosis early after radiation therapy (1 Gy) which progressed to necrosis in a unique in vivo model of gliosarcoma that may prove useful in determining new therapeutic approaches to radiation therapy and tumor cell biology.

Original languageEnglish (US)
Pages (from-to)142-148
Number of pages7
JournalMinimally Invasive Neurosurgery
Volume48
Issue number3
DOIs
StatePublished - Jun 1 2005
Externally publishedYes

Fingerprint

Gliosarcoma
Radiosurgery
Cell Death
Radiation Dosage
Apoptosis
Radiation
Radiotherapy
Trypan Blue
Neoplasms
Necrosis
Caspase 8
Propidium
Caudate Nucleus
Annexin A5
Glioma
Caspase 3
Cell Biology
Flow Cytometry
Staining and Labeling
Cell Line

Keywords

  • 9L gliosarcoma
  • Apoptosis
  • Cell death
  • Radiosurgery

ASJC Scopus subject areas

  • Surgery
  • Clinical Neurology

Cite this

Psarros, T. G., Mickey, B., Gilio, J., Drees, J., Gall, K., Carlson, D., ... Willis, M. S. (2005). Gliosarcoma cell death after radiosurgery in a rat model. Minimally Invasive Neurosurgery, 48(3), 142-148. https://doi.org/10.1055/s-2004-830266

Gliosarcoma cell death after radiosurgery in a rat model. / Psarros, Tom G.; Mickey, B.; Gilio, J.; Drees, J.; Gall, K.; Carlson, D.; Giller, Cole A.; Willis, M. S.

In: Minimally Invasive Neurosurgery, Vol. 48, No. 3, 01.06.2005, p. 142-148.

Research output: Contribution to journalArticle

Psarros, TG, Mickey, B, Gilio, J, Drees, J, Gall, K, Carlson, D, Giller, CA & Willis, MS 2005, 'Gliosarcoma cell death after radiosurgery in a rat model', Minimally Invasive Neurosurgery, vol. 48, no. 3, pp. 142-148. https://doi.org/10.1055/s-2004-830266
Psarros TG, Mickey B, Gilio J, Drees J, Gall K, Carlson D et al. Gliosarcoma cell death after radiosurgery in a rat model. Minimally Invasive Neurosurgery. 2005 Jun 1;48(3):142-148. https://doi.org/10.1055/s-2004-830266
Psarros, Tom G. ; Mickey, B. ; Gilio, J. ; Drees, J. ; Gall, K. ; Carlson, D. ; Giller, Cole A. ; Willis, M. S. / Gliosarcoma cell death after radiosurgery in a rat model. In: Minimally Invasive Neurosurgery. 2005 ; Vol. 48, No. 3. pp. 142-148.
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