Glucose-dependent insulinotropic peptide stimulates proliferation and TGF-β release from MG-63 cells

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Glucose-dependent insulinotropic peptide (GIP) is known to modulate alkaline phosphatase activity and collagen type I message in osteoblastic-like cells. GIP effects on cell proliferation are not known. We report that GIP dose dependently stimulated 3 H-thymidine incorporation in the osteoblastic-like cell line MG-63. Furthermore, GIP increased message and secretion of transforming growth factor beta (TGF-β), an agent known to regulate osteoblastic proliferation and differentiation. However, when GIP was added to MG-63 cells concurrently with a TGF-β neutralizing antibody, there was no effect on 3 H-thymidine incorporation in these cells. These data demonstrate that GIP stimulates osteoblastic-like cell proliferation but that this effect is not mediated by TGF-β.

Original languageEnglish (US)
Pages (from-to)611-616
Number of pages6
JournalPeptides
Volume24
Issue number4
DOIs
StatePublished - Apr 1 2003

Fingerprint

Gastric Inhibitory Polypeptide
Transforming Growth Factor beta
Cell proliferation
Thymidine
Cell Proliferation
Collagen Type I
Neutralizing Antibodies
Alkaline Phosphatase
Cells
Cell Line

Keywords

  • GIP
  • Incretin
  • Osteoblast
  • TGF-β

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

Cite this

@article{54fb9644596f46b79498b3a3476f89ba,
title = "Glucose-dependent insulinotropic peptide stimulates proliferation and TGF-β release from MG-63 cells",
abstract = "Glucose-dependent insulinotropic peptide (GIP) is known to modulate alkaline phosphatase activity and collagen type I message in osteoblastic-like cells. GIP effects on cell proliferation are not known. We report that GIP dose dependently stimulated 3 H-thymidine incorporation in the osteoblastic-like cell line MG-63. Furthermore, GIP increased message and secretion of transforming growth factor beta (TGF-β), an agent known to regulate osteoblastic proliferation and differentiation. However, when GIP was added to MG-63 cells concurrently with a TGF-β neutralizing antibody, there was no effect on 3 H-thymidine incorporation in these cells. These data demonstrate that GIP stimulates osteoblastic-like cell proliferation but that this effect is not mediated by TGF-β.",
keywords = "GIP, Incretin, Osteoblast, TGF-β",
author = "Qing Zhong and Ding, {Ke Hong} and Mulloy, {Anthony L.} and Bollag, {Roni J.} and Isales, {Carlos M.}",
year = "2003",
month = "4",
day = "1",
doi = "10.1016/S0196-9781(03)00103-7",
language = "English (US)",
volume = "24",
pages = "611--616",
journal = "Peptides",
issn = "0196-9781",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Glucose-dependent insulinotropic peptide stimulates proliferation and TGF-β release from MG-63 cells

AU - Zhong, Qing

AU - Ding, Ke Hong

AU - Mulloy, Anthony L.

AU - Bollag, Roni J.

AU - Isales, Carlos M.

PY - 2003/4/1

Y1 - 2003/4/1

N2 - Glucose-dependent insulinotropic peptide (GIP) is known to modulate alkaline phosphatase activity and collagen type I message in osteoblastic-like cells. GIP effects on cell proliferation are not known. We report that GIP dose dependently stimulated 3 H-thymidine incorporation in the osteoblastic-like cell line MG-63. Furthermore, GIP increased message and secretion of transforming growth factor beta (TGF-β), an agent known to regulate osteoblastic proliferation and differentiation. However, when GIP was added to MG-63 cells concurrently with a TGF-β neutralizing antibody, there was no effect on 3 H-thymidine incorporation in these cells. These data demonstrate that GIP stimulates osteoblastic-like cell proliferation but that this effect is not mediated by TGF-β.

AB - Glucose-dependent insulinotropic peptide (GIP) is known to modulate alkaline phosphatase activity and collagen type I message in osteoblastic-like cells. GIP effects on cell proliferation are not known. We report that GIP dose dependently stimulated 3 H-thymidine incorporation in the osteoblastic-like cell line MG-63. Furthermore, GIP increased message and secretion of transforming growth factor beta (TGF-β), an agent known to regulate osteoblastic proliferation and differentiation. However, when GIP was added to MG-63 cells concurrently with a TGF-β neutralizing antibody, there was no effect on 3 H-thymidine incorporation in these cells. These data demonstrate that GIP stimulates osteoblastic-like cell proliferation but that this effect is not mediated by TGF-β.

KW - GIP

KW - Incretin

KW - Osteoblast

KW - TGF-β

UR - http://www.scopus.com/inward/record.url?scp=0037629452&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037629452&partnerID=8YFLogxK

U2 - 10.1016/S0196-9781(03)00103-7

DO - 10.1016/S0196-9781(03)00103-7

M3 - Article

VL - 24

SP - 611

EP - 616

JO - Peptides

JF - Peptides

SN - 0196-9781

IS - 4

ER -