Glutathione S-transferases of female A/J mouse liver and forestomach and their differential induction by anti-carcinogenic organosulfides from garlic

Xun Hu, Patrick J. Benson, Sanjay K. Srivastava, Lisa M. Mack, Hong Xia, Vicram Gupta, Howard A. Zaren, Shivendra V. Singh

Research output: Contribution to journalArticle

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Abstract

This study characterizes glutathione (GSH) S-transferase (GST) isoenzymes of the liver and forestomach of the female A/J mouse and compares their specificities in catalyzing the conjugation of GSH with 7β,8α- dihydroxy-9α,10α-oxy-7,8,9,10-tetrahydrobenzo[a] pyrene (anti-BPDE), the ultimate carcinogenic metabolite of benzo[a]pyrene (BP). The GST activity in female A/J mouse liver was expressed by a minimum of seven isoenzymes which arose from different homo- or heterodimeric combinations of at least two α class (designated as α1 and α4), four μ class (μ1 to μ4), and one π class GST subunit. The GST isoenzyme composition of A/J mouse forestomach appeared to be different from that of the liver. For example, while GST isoenzymes containing μ3 and μ4 type subunits were selectively expressed in the liver, an α class heterodimeric GST isoenzyme (containing α2 and α3 subunits) was expressed in the forestomach but could not be detected in the liver. The (+)-anti-BPDE appeared to be a better substrate than the (-)- enantiomer for all GSTs, except for isoenzymes containing the α4 type GST subunit. The murine π class GST isoenzyme displayed relativey higher specific activity toward (+)-anti-BPDE compared to other GSTs. The specific activities of mouse GSTs toward (+)-anti-BPDE were in the order of π > μ > α. These results suggest that the π class GST isoenzyme may play an important role in providing protection against BP-induced cancer. Therefore, it seems logical to postulate that the ability of a chemoprotector to increase the expression of GST π may be an important determinant of its effectiveness against BP-induced cancer. To test the validity of this contention, we have determined the effects on hepatic and forestomach GST isoenzyme/subunit expression of three naturally occurring organosulfides (OSCs) from garlic, which significantly differ in their effectiveness against BP-induced forestomach cancer. Treatment of mice with diallyl sulfide (DAS) and diallyl trisulfide (DATS), which are potent inhibitors of BP-induced forestomach cancer in mice, resulted in a significant increase in hepatic and forestomach GST activity toward anti-BPDE. On the contrary, this activity was not increased in either organ by dipropyl sulfide (DPS), which is ineffective against BP-induced forestomach cancer. The chemopreventive efficacy of these OSCs correlated with their ability to increase the expression of GST π. For example, DAS treatment resulted in approximate increases of 1.7- and 2.2- fold in hepatic and forestomach GST π expression, respectively, over the control. Treatment of mice with DATS, which is a relatively more potent inhibitor of BP-induced forestomach cancer than DAS, resulted in about 3.8- and 3.2-fold increases, respectively, in hepatic and forestomach GST π expression over the control. On the contrary, the expression of hepatic and forestomach GST was increased only marginally (10-20%) upon DPS administration. In conclusion, the results of the present study suggest that induction of GST π can be used as a bioassay for screening potential inhibitors of BP-induced cancer.

Original languageEnglish (US)
Pages (from-to)199-214
Number of pages16
JournalArchives of Biochemistry and Biophysics
Volume336
Issue number2
DOIs
StatePublished - Dec 15 1996

Fingerprint

Garlic
Transferases
Glutathione Transferase
Liver
Isoenzymes
7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
Neoplasms
Benzo(a)pyrene
Enantiomers
Bioassay

Keywords

  • benzo(a)pyrene
  • carcinogenesis
  • chemoprevention
  • garlic
  • glutathione S- transferase
  • organosulfides

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Glutathione S-transferases of female A/J mouse liver and forestomach and their differential induction by anti-carcinogenic organosulfides from garlic. / Hu, Xun; Benson, Patrick J.; Srivastava, Sanjay K.; Mack, Lisa M.; Xia, Hong; Gupta, Vicram; Zaren, Howard A.; Singh, Shivendra V.

In: Archives of Biochemistry and Biophysics, Vol. 336, No. 2, 15.12.1996, p. 199-214.

Research output: Contribution to journalArticle

Hu, Xun ; Benson, Patrick J. ; Srivastava, Sanjay K. ; Mack, Lisa M. ; Xia, Hong ; Gupta, Vicram ; Zaren, Howard A. ; Singh, Shivendra V. / Glutathione S-transferases of female A/J mouse liver and forestomach and their differential induction by anti-carcinogenic organosulfides from garlic. In: Archives of Biochemistry and Biophysics. 1996 ; Vol. 336, No. 2. pp. 199-214.
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T1 - Glutathione S-transferases of female A/J mouse liver and forestomach and their differential induction by anti-carcinogenic organosulfides from garlic

AU - Hu, Xun

AU - Benson, Patrick J.

AU - Srivastava, Sanjay K.

AU - Mack, Lisa M.

AU - Xia, Hong

AU - Gupta, Vicram

AU - Zaren, Howard A.

AU - Singh, Shivendra V.

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N2 - This study characterizes glutathione (GSH) S-transferase (GST) isoenzymes of the liver and forestomach of the female A/J mouse and compares their specificities in catalyzing the conjugation of GSH with 7β,8α- dihydroxy-9α,10α-oxy-7,8,9,10-tetrahydrobenzo[a] pyrene (anti-BPDE), the ultimate carcinogenic metabolite of benzo[a]pyrene (BP). The GST activity in female A/J mouse liver was expressed by a minimum of seven isoenzymes which arose from different homo- or heterodimeric combinations of at least two α class (designated as α1 and α4), four μ class (μ1 to μ4), and one π class GST subunit. The GST isoenzyme composition of A/J mouse forestomach appeared to be different from that of the liver. For example, while GST isoenzymes containing μ3 and μ4 type subunits were selectively expressed in the liver, an α class heterodimeric GST isoenzyme (containing α2 and α3 subunits) was expressed in the forestomach but could not be detected in the liver. The (+)-anti-BPDE appeared to be a better substrate than the (-)- enantiomer for all GSTs, except for isoenzymes containing the α4 type GST subunit. The murine π class GST isoenzyme displayed relativey higher specific activity toward (+)-anti-BPDE compared to other GSTs. The specific activities of mouse GSTs toward (+)-anti-BPDE were in the order of π > μ > α. These results suggest that the π class GST isoenzyme may play an important role in providing protection against BP-induced cancer. Therefore, it seems logical to postulate that the ability of a chemoprotector to increase the expression of GST π may be an important determinant of its effectiveness against BP-induced cancer. To test the validity of this contention, we have determined the effects on hepatic and forestomach GST isoenzyme/subunit expression of three naturally occurring organosulfides (OSCs) from garlic, which significantly differ in their effectiveness against BP-induced forestomach cancer. Treatment of mice with diallyl sulfide (DAS) and diallyl trisulfide (DATS), which are potent inhibitors of BP-induced forestomach cancer in mice, resulted in a significant increase in hepatic and forestomach GST activity toward anti-BPDE. On the contrary, this activity was not increased in either organ by dipropyl sulfide (DPS), which is ineffective against BP-induced forestomach cancer. The chemopreventive efficacy of these OSCs correlated with their ability to increase the expression of GST π. For example, DAS treatment resulted in approximate increases of 1.7- and 2.2- fold in hepatic and forestomach GST π expression, respectively, over the control. Treatment of mice with DATS, which is a relatively more potent inhibitor of BP-induced forestomach cancer than DAS, resulted in about 3.8- and 3.2-fold increases, respectively, in hepatic and forestomach GST π expression over the control. On the contrary, the expression of hepatic and forestomach GST was increased only marginally (10-20%) upon DPS administration. In conclusion, the results of the present study suggest that induction of GST π can be used as a bioassay for screening potential inhibitors of BP-induced cancer.

AB - This study characterizes glutathione (GSH) S-transferase (GST) isoenzymes of the liver and forestomach of the female A/J mouse and compares their specificities in catalyzing the conjugation of GSH with 7β,8α- dihydroxy-9α,10α-oxy-7,8,9,10-tetrahydrobenzo[a] pyrene (anti-BPDE), the ultimate carcinogenic metabolite of benzo[a]pyrene (BP). The GST activity in female A/J mouse liver was expressed by a minimum of seven isoenzymes which arose from different homo- or heterodimeric combinations of at least two α class (designated as α1 and α4), four μ class (μ1 to μ4), and one π class GST subunit. The GST isoenzyme composition of A/J mouse forestomach appeared to be different from that of the liver. For example, while GST isoenzymes containing μ3 and μ4 type subunits were selectively expressed in the liver, an α class heterodimeric GST isoenzyme (containing α2 and α3 subunits) was expressed in the forestomach but could not be detected in the liver. The (+)-anti-BPDE appeared to be a better substrate than the (-)- enantiomer for all GSTs, except for isoenzymes containing the α4 type GST subunit. The murine π class GST isoenzyme displayed relativey higher specific activity toward (+)-anti-BPDE compared to other GSTs. The specific activities of mouse GSTs toward (+)-anti-BPDE were in the order of π > μ > α. These results suggest that the π class GST isoenzyme may play an important role in providing protection against BP-induced cancer. Therefore, it seems logical to postulate that the ability of a chemoprotector to increase the expression of GST π may be an important determinant of its effectiveness against BP-induced cancer. To test the validity of this contention, we have determined the effects on hepatic and forestomach GST isoenzyme/subunit expression of three naturally occurring organosulfides (OSCs) from garlic, which significantly differ in their effectiveness against BP-induced forestomach cancer. Treatment of mice with diallyl sulfide (DAS) and diallyl trisulfide (DATS), which are potent inhibitors of BP-induced forestomach cancer in mice, resulted in a significant increase in hepatic and forestomach GST activity toward anti-BPDE. On the contrary, this activity was not increased in either organ by dipropyl sulfide (DPS), which is ineffective against BP-induced forestomach cancer. The chemopreventive efficacy of these OSCs correlated with their ability to increase the expression of GST π. For example, DAS treatment resulted in approximate increases of 1.7- and 2.2- fold in hepatic and forestomach GST π expression, respectively, over the control. Treatment of mice with DATS, which is a relatively more potent inhibitor of BP-induced forestomach cancer than DAS, resulted in about 3.8- and 3.2-fold increases, respectively, in hepatic and forestomach GST π expression over the control. On the contrary, the expression of hepatic and forestomach GST was increased only marginally (10-20%) upon DPS administration. In conclusion, the results of the present study suggest that induction of GST π can be used as a bioassay for screening potential inhibitors of BP-induced cancer.

KW - benzo(a)pyrene

KW - carcinogenesis

KW - chemoprevention

KW - garlic

KW - glutathione S- transferase

KW - organosulfides

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