TY - JOUR
T1 - Glycosyltransferase activities in cultured endothelial cells of bovine brain microvascular origin
AU - Kanda, Takashi
AU - Ariga, Toshio
AU - Yamawaki, Masanaga
AU - Yoshino, Hiide
AU - Gu, Xin Bin
AU - Yu, Robert K.
N1 - Funding Information:
This work was supported by USPHS grants NS 11853, NS 26994 and a grant (RG 2690A6/2) from the National Multiple Sclerosis Society.
PY - 1997
Y1 - 1997
N2 - Bovine brain microvascular endothelial cells (BMECs) express GM3 (NeuAc) and GM3 (NeuGc) as the major gangliosides, and GM1, GD1a, GD1b, GT1b as well as sialosylparagloboside and sialosyllactosaminylparagloboside as the minor species. To investigate the metabolic basis of this ganglioside pattern, the activities of eight glycosyltransferases (GM3-, GD1a-, GD3-, LM1-, GM2 (NeuAc)-, GM2 (NeuGc)-, LacCer-, and GM1-synthases) in cultured BMECs were studied. It was found that BMECs possessed high activities of GM3- and GDla- synthases, and low activities of GM2-, GM1-, and GD3-synthases. Thus, the present study provides evidence that endothelial cells are capable of synthesizing gangliosides in situ and that the high content of GM3 in BMEC is closely associated with high activities of GM3-synthase and low activities of GM2-, GM1-, and GD3-synthases.
AB - Bovine brain microvascular endothelial cells (BMECs) express GM3 (NeuAc) and GM3 (NeuGc) as the major gangliosides, and GM1, GD1a, GD1b, GT1b as well as sialosylparagloboside and sialosyllactosaminylparagloboside as the minor species. To investigate the metabolic basis of this ganglioside pattern, the activities of eight glycosyltransferases (GM3-, GD1a-, GD3-, LM1-, GM2 (NeuAc)-, GM2 (NeuGc)-, LacCer-, and GM1-synthases) in cultured BMECs were studied. It was found that BMECs possessed high activities of GM3- and GDla- synthases, and low activities of GM2-, GM1-, and GD3-synthases. Thus, the present study provides evidence that endothelial cells are capable of synthesizing gangliosides in situ and that the high content of GM3 in BMEC is closely associated with high activities of GM3-synthase and low activities of GM2-, GM1-, and GD3-synthases.
KW - Glycosyltransferase
KW - endothelial cells
KW - gangliosides
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U2 - 10.1023/A:1027363828172
DO - 10.1023/A:1027363828172
M3 - Article
C2 - 9130257
AN - SCOPUS:0030999130
SN - 0364-3190
VL - 22
SP - 463
EP - 466
JO - Neurochemical Research
JF - Neurochemical Research
IS - 4
ER -