GM1 ganglioside is involved in epigenetic activation loci of neuronal cells

Yi Tzang Tsai, Yutaka Itokazu, Robert K Yu

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Gangliosides are sialic acid-containing glycosphingolipids that are most abundant in the nerve tissues. The quantity and expression pattern of gangliosides in brain change drastically throughout development and are mainly regulated through stage-specific expression of glycosyltransferase (ganglioside synthase) genes. We previously demonstrated that acetylation of histones H3 and H4 on the N-acetylgalactosaminyltransferase I (GalNAcT, GA2/GM2/GD2/GT2-synthase) gene promoter resulted in recruitment of trans-activation factors. In addition, we reported that epigenetic activation of the GalNAcT gene was also detected as accompanied by an apparent induction of neuronal differentiation in neural stem cells responding to an exogenous supplement of ganglioside GM1. Here, we present evidence supporting the concept that nuclear GM1 is associated with gene regulation in neuronal cells. We found that nuclear GM1 binds acetylated histones on the promoters of the GalNAcT and NeuroD1 genes in differentiated neurons. Our study demonstrates for the first time that GM1 interacts with chromatin via acetylated histones at the nuclear periphery of neuronal cells.

Original languageEnglish (US)
Pages (from-to)107-115
Number of pages9
JournalNeurochemical Research
Volume41
Issue number1-2
DOIs
StatePublished - Oct 24 2016

Fingerprint

G(M1) Ganglioside
Epigenomics
Histones
Gangliosides
Genes
Chemical activation
(N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase
N-Acetylgalactosaminyltransferases
Nerve Tissue
Acetylation
Glycosphingolipids
Glycosyltransferases
Neural Stem Cells
N-Acetylneuraminic Acid
Stem cells
Gene expression
Transcriptional Activation
Neurons
Chromatin
Brain

Keywords

  • Brain development
  • Epigenetic regulation
  • GM1 ganglioside
  • Glycosyltransferase
  • Histone

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

GM1 ganglioside is involved in epigenetic activation loci of neuronal cells. / Tsai, Yi Tzang; Itokazu, Yutaka; Yu, Robert K.

In: Neurochemical Research, Vol. 41, No. 1-2, 24.10.2016, p. 107-115.

Research output: Contribution to journalArticle

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