OBJECTIVE: To determine the influence of pituitary gonadotropins, which increase dramatically in concentration at ovulation and in the early years of the postmenopausal transition, on inflammatory cytokine production. DESIGN: Cross-sectional population sampling, in vitro experimentation. PARTICIPANTS: Healthy subjects, including six men, five women between the ages of 18 and 35 years, and four women who were four to 20 years past menopause. METHOD: Isolated peripheral blood mononuclear cells were incubated with physiological concentrations of follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The concentrations of interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6) secreted into the supernatants were measured by enzyme-linked immunosorbent assays. RESULTS: Under basal conditions, FSH stimulated IL-1 beta secretion by cells isolated from women in the follicular phase. Under conditions of cellular activation with bacterial lipopolysaccharide, FSH and LH interacted to inhibit IL-1 beta secretion by cells isolated from all groups. The gonadotropins had no significant influence on IL-6 secretion regardless of donor group or cellular activation state. CONCLUSION: The results of this study support the concept that gonadotropins may contribute to the changes in IL-1 beta secretion that occur at the periovulatory and postmenopausal periods.
|Original language||English (US)|
|Number of pages||5|
|Journal||The journal of gender-specific medicine : JGSM : the official journal of the Partnership for Women's Health at Columbia|
|State||Published - 1999|
ASJC Scopus subject areas
- Pathology and Forensic Medicine