Gonococcal transferrin-binding protein 1 is required for transferrin utilization and is homologous to tonB-dependent outer membrane receptors

C. N. Cornelissen, G. D. Biswas, J. Tsai, D. K. Paruchuri, Stuart A Thompson, P. F. Sparling

Research output: Contribution to journalArticle

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Abstract

The pathogenic Neisseria species are capable of utilizing transferrin as their sole source of iron. A neisserial transferrin receptor has been identified and its characteristics defined; however, the biochemical identities of proteins which are required for transferrin receptor function have not yet been determined. We identified two iron-repressible transferrin- binding proteins in Neisseria gonorrhoeae, TBP1 and TBP2. Two approaches were taken to clone genes required for gonococcal transferrin receptor function. First, polyclonal antiserum raised against TBP1 was used to identify clones expressing TBP1 epitopes. Second, a wild-type gene copy was cloned that repaired the defect in a transferrin receptor function (trf) mutant. The clones obtained by these two approaches were shown to overlap by DNA sequencing. Transposon mutagenesis of both clones and recombination of mutagenized fragments into the gonococcal chromosome generated mutants that showed reduced binding of transferrin to whole cells and that were incapable of growth on transferrin. No TBP1 was produced in these mutants, but TBP2 expression was normal. The DNA sequence of the gene encoding gonococcal TBP1 (tbpA) predicted a protein sequence homologous to the Escherichia coli and Pseudomonas putida TonB-dependent outer membrane receptors. Thus, both the function and the predicted protein sequence of TBP1 were consistent with this protein serving as a transferrin receptor.

Original languageEnglish (US)
Pages (from-to)5788-5797
Number of pages10
JournalJournal of Bacteriology
Volume174
Issue number18
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

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Transferrin-Binding Proteins
Transferrin Receptors
Transferrin
Clone Cells
Membranes
Proteins
Iron
Genes
Neisseria
Pseudomonas putida
Neisseria gonorrhoeae
Sequence Homology
DNA Sequence Analysis
Mutagenesis
Genetic Recombination
Immune Sera
Epitopes
Chromosomes
Escherichia coli
Growth

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Gonococcal transferrin-binding protein 1 is required for transferrin utilization and is homologous to tonB-dependent outer membrane receptors. / Cornelissen, C. N.; Biswas, G. D.; Tsai, J.; Paruchuri, D. K.; Thompson, Stuart A; Sparling, P. F.

In: Journal of Bacteriology, Vol. 174, No. 18, 01.01.1992, p. 5788-5797.

Research output: Contribution to journalArticle

Cornelissen, C. N. ; Biswas, G. D. ; Tsai, J. ; Paruchuri, D. K. ; Thompson, Stuart A ; Sparling, P. F. / Gonococcal transferrin-binding protein 1 is required for transferrin utilization and is homologous to tonB-dependent outer membrane receptors. In: Journal of Bacteriology. 1992 ; Vol. 174, No. 18. pp. 5788-5797.
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