GPR30 mediates estrogen rapid signaling and neuroprotection

Hui Tang, Quanguang Zhang, Licai Yang, Yan Dong, Mohammad Badruzzaman Khan, Fang Yang, Darrell W Brann, Ruimin Wang

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

G-protein-coupled estrogen receptor-30 (GPR30), also known as G-protein estrogen receptor-1 (GPER1), is a putative extranuclear estrogen receptor whose precise functions in the brain are poorly understood. Studies using exogenous administration of the GPR30 agonist, G1 suggests that GPR30 may have a neuroprotective role in cerebral ischemia. However, the physiological role of GPR30 in mediating estrogen (E2)-induced neuroprotection in cerebral ischemia remains unclear. Also unclear is whether GPR30 has a role in mediating rapid signaling by E2 after cerebral ischemia, which is thought to underlie its neuroprotective actions. To address these deficits in our knowledge, the current study examined the effect of antisense oligonucleotide (AS) knockdown of GPR30 in the hippocampal CA1 region upon E2-BSA-induced neuroprotection and rapid kinase signaling in a rat model of global cerebral ischemia (GCI). Immunohistochemistry demonstrated that GPR30 is strongly expressed in the hippocampal CA1 region and dentate gyrus, with less expression in the CA3 region. E2-BSA exerted robust neuroprotection of hippocampal CA1 neurons against GCI, an effect abrogated by AS knockdown of GPR30. Missense control oligonucleotides had no effect upon E2-BSA-induced neuroprotection, indicating specificity of the effect. The GPR30 agonist, G1 also exerted significant neuroprotection against GCI. E2-BSA and G1 also rapidly enhanced activation of the prosurvival kinases, Akt and ERK, while decreasing proapototic JNK activation. Importantly, AS knockdown of GPR30 markedly attenuated these rapid kinase signaling effects of E2-BSA. As a whole, the studies provide evidence of an important role of GPR30 in mediating the rapid signaling and neuroprotective actions of E2 in the hippocampus.

Original languageEnglish (US)
Pages (from-to)52-58
Number of pages7
JournalMolecular and Cellular Endocrinology
Volume387
Issue number1-2
DOIs
StatePublished - Apr 25 2014

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G-Protein-Coupled Receptors
GTP-Binding Proteins
Estrogen Receptors
Estrogens
Brain Ischemia
Antisense Oligonucleotides
Hippocampal CA1 Region
Phosphotransferases
Neuroprotection
Chemical activation
Estrogen Receptor alpha
Dentate Gyrus
Oligonucleotides
Hippocampus
Neurons
Rats
Brain
Immunohistochemistry
estradiol-bovine serum albumin

Keywords

  • Estradiol
  • Estrogen
  • GPER1
  • GPR30
  • Neuroprotection

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

GPR30 mediates estrogen rapid signaling and neuroprotection. / Tang, Hui; Zhang, Quanguang; Yang, Licai; Dong, Yan; Khan, Mohammad Badruzzaman; Yang, Fang; Brann, Darrell W; Wang, Ruimin.

In: Molecular and Cellular Endocrinology, Vol. 387, No. 1-2, 25.04.2014, p. 52-58.

Research output: Contribution to journalArticle

Tang, Hui ; Zhang, Quanguang ; Yang, Licai ; Dong, Yan ; Khan, Mohammad Badruzzaman ; Yang, Fang ; Brann, Darrell W ; Wang, Ruimin. / GPR30 mediates estrogen rapid signaling and neuroprotection. In: Molecular and Cellular Endocrinology. 2014 ; Vol. 387, No. 1-2. pp. 52-58.
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AU - Zhang, Quanguang

AU - Yang, Licai

AU - Dong, Yan

AU - Khan, Mohammad Badruzzaman

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AU - Brann, Darrell W

AU - Wang, Ruimin

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KW - Estrogen

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