Granulocyte colony-stimulating factor administration alters femoral biomechanical properties in C57BL/6 mice

Yii Der Wu, Chi Hui Chien, Yuh J. Chao, Mark W Hamrick, William D. Hill, Jack C Yu, Xiaodong Li

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The clinical application of G-CSF is broadening. In addition to treating neutropenia and in bone marrow transplants, it is now being considered for functional recovery after myocardial infarction and stroke. It is thus very important that the effects of extended G-CSF administration on the skeleton are investigated. To simulate this potential clinical use of G-CSF in postmyocardial infarction or cerebral vascular accident, a 2-week course of administration was selected. Ten C57BL/6 mice at 22 weeks of age were given intraperitoneal injection of saline, and another nine of the same age were given G-CSF. Four weeks later, femurs were harvested and three-point-bend tests were performed until fracture. From the load-displacement curve recorded during the test, the stiffness, Young's modulus, fracture strength of the bone, fracture energy, and the total energy to break the femur were determined. The test data show that mice treated with G-CSF have significantly lower modulus in their femurs when compared to the controlled mice treated with saline. The stiffness demonstrates the largest decrease, by as much as 25%. As its clinical use increases, G-CSF effects on the mechanical properties of the skeleton become increasingly more important because many of these diseases occur in older patients with already compromised skeleton by osteopenia or osteoporosis. How G-CSF administration achieves these alterations in skeletal biomechanical properties is unclear. Although the current findings confirm its known temporary catabolic effects on bone homeostasis, it also suggests that a transient state of higher bone compliance following the end of G-CSF administration can be achieved that may have clinical benefits.

Original languageEnglish (US)
Pages (from-to)972-979
Number of pages8
JournalJournal of Biomedical Materials Research - Part A
Volume87
Issue number4
DOIs
StatePublished - Dec 15 2008

Fingerprint

Granulocyte Colony-Stimulating Factor
Thigh
Inbred C57BL Mouse
Bone
Stiffness
Skeleton
Femur
Transplants
Fracture energy
Fracture toughness
Loads (forces)
Accidents
Elastic moduli
Bone and Bones
Recovery
Mechanical properties
Metabolic Bone Diseases
Elastic Modulus
Bone Fractures
Cerebral Infarction

Keywords

  • Bone strength
  • C57BL/6 mice
  • G-CSF
  • Hind limb

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

Granulocyte colony-stimulating factor administration alters femoral biomechanical properties in C57BL/6 mice. / Wu, Yii Der; Chien, Chi Hui; Chao, Yuh J.; Hamrick, Mark W; Hill, William D.; Yu, Jack C; Li, Xiaodong.

In: Journal of Biomedical Materials Research - Part A, Vol. 87, No. 4, 15.12.2008, p. 972-979.

Research output: Contribution to journalArticle

@article{abe85c8d4b024e56a950b77d36094905,
title = "Granulocyte colony-stimulating factor administration alters femoral biomechanical properties in C57BL/6 mice",
abstract = "The clinical application of G-CSF is broadening. In addition to treating neutropenia and in bone marrow transplants, it is now being considered for functional recovery after myocardial infarction and stroke. It is thus very important that the effects of extended G-CSF administration on the skeleton are investigated. To simulate this potential clinical use of G-CSF in postmyocardial infarction or cerebral vascular accident, a 2-week course of administration was selected. Ten C57BL/6 mice at 22 weeks of age were given intraperitoneal injection of saline, and another nine of the same age were given G-CSF. Four weeks later, femurs were harvested and three-point-bend tests were performed until fracture. From the load-displacement curve recorded during the test, the stiffness, Young's modulus, fracture strength of the bone, fracture energy, and the total energy to break the femur were determined. The test data show that mice treated with G-CSF have significantly lower modulus in their femurs when compared to the controlled mice treated with saline. The stiffness demonstrates the largest decrease, by as much as 25{\%}. As its clinical use increases, G-CSF effects on the mechanical properties of the skeleton become increasingly more important because many of these diseases occur in older patients with already compromised skeleton by osteopenia or osteoporosis. How G-CSF administration achieves these alterations in skeletal biomechanical properties is unclear. Although the current findings confirm its known temporary catabolic effects on bone homeostasis, it also suggests that a transient state of higher bone compliance following the end of G-CSF administration can be achieved that may have clinical benefits.",
keywords = "Bone strength, C57BL/6 mice, G-CSF, Hind limb",
author = "Wu, {Yii Der} and Chien, {Chi Hui} and Chao, {Yuh J.} and Hamrick, {Mark W} and Hill, {William D.} and Yu, {Jack C} and Xiaodong Li",
year = "2008",
month = "12",
day = "15",
doi = "10.1002/jbm.a.31840",
language = "English (US)",
volume = "87",
pages = "972--979",
journal = "Journal of Biomedical Materials Research - Part A",
issn = "0021-9304",
publisher = "Heterocorporation",
number = "4",

}

TY - JOUR

T1 - Granulocyte colony-stimulating factor administration alters femoral biomechanical properties in C57BL/6 mice

AU - Wu, Yii Der

AU - Chien, Chi Hui

AU - Chao, Yuh J.

AU - Hamrick, Mark W

AU - Hill, William D.

AU - Yu, Jack C

AU - Li, Xiaodong

PY - 2008/12/15

Y1 - 2008/12/15

N2 - The clinical application of G-CSF is broadening. In addition to treating neutropenia and in bone marrow transplants, it is now being considered for functional recovery after myocardial infarction and stroke. It is thus very important that the effects of extended G-CSF administration on the skeleton are investigated. To simulate this potential clinical use of G-CSF in postmyocardial infarction or cerebral vascular accident, a 2-week course of administration was selected. Ten C57BL/6 mice at 22 weeks of age were given intraperitoneal injection of saline, and another nine of the same age were given G-CSF. Four weeks later, femurs were harvested and three-point-bend tests were performed until fracture. From the load-displacement curve recorded during the test, the stiffness, Young's modulus, fracture strength of the bone, fracture energy, and the total energy to break the femur were determined. The test data show that mice treated with G-CSF have significantly lower modulus in their femurs when compared to the controlled mice treated with saline. The stiffness demonstrates the largest decrease, by as much as 25%. As its clinical use increases, G-CSF effects on the mechanical properties of the skeleton become increasingly more important because many of these diseases occur in older patients with already compromised skeleton by osteopenia or osteoporosis. How G-CSF administration achieves these alterations in skeletal biomechanical properties is unclear. Although the current findings confirm its known temporary catabolic effects on bone homeostasis, it also suggests that a transient state of higher bone compliance following the end of G-CSF administration can be achieved that may have clinical benefits.

AB - The clinical application of G-CSF is broadening. In addition to treating neutropenia and in bone marrow transplants, it is now being considered for functional recovery after myocardial infarction and stroke. It is thus very important that the effects of extended G-CSF administration on the skeleton are investigated. To simulate this potential clinical use of G-CSF in postmyocardial infarction or cerebral vascular accident, a 2-week course of administration was selected. Ten C57BL/6 mice at 22 weeks of age were given intraperitoneal injection of saline, and another nine of the same age were given G-CSF. Four weeks later, femurs were harvested and three-point-bend tests were performed until fracture. From the load-displacement curve recorded during the test, the stiffness, Young's modulus, fracture strength of the bone, fracture energy, and the total energy to break the femur were determined. The test data show that mice treated with G-CSF have significantly lower modulus in their femurs when compared to the controlled mice treated with saline. The stiffness demonstrates the largest decrease, by as much as 25%. As its clinical use increases, G-CSF effects on the mechanical properties of the skeleton become increasingly more important because many of these diseases occur in older patients with already compromised skeleton by osteopenia or osteoporosis. How G-CSF administration achieves these alterations in skeletal biomechanical properties is unclear. Although the current findings confirm its known temporary catabolic effects on bone homeostasis, it also suggests that a transient state of higher bone compliance following the end of G-CSF administration can be achieved that may have clinical benefits.

KW - Bone strength

KW - C57BL/6 mice

KW - G-CSF

KW - Hind limb

UR - http://www.scopus.com/inward/record.url?scp=56749185579&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=56749185579&partnerID=8YFLogxK

U2 - 10.1002/jbm.a.31840

DO - 10.1002/jbm.a.31840

M3 - Article

C2 - 18257084

AN - SCOPUS:56749185579

VL - 87

SP - 972

EP - 979

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

SN - 0021-9304

IS - 4

ER -