Granulosa cell expression of G1/S phase cyclins and cyclin-dependent kinases in PMSG-induced follicle growth

Jennifer D Cannon, Mary Cherian-Shaw, Tara Lovekamp-Swan, Charles L. Chaffin

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Follicular development involves a complex orchestration of granulosa cell proliferation and differentiation. It is becoming increasingly apparent that the rate of granulosa cell proliferation declines as follicles reach the large antral status, prior to an ovulatory gonadotropin stimulus, although a precise time course and mechanism for this decline has not been described. The goal of the present study was to characterize granulosa cell proliferation following the onset of antral follicle growth in PMSG-primed immature rats, with emphasis on G1/S phase cyclins and cyclin-dependent kinases. Flow cytometric analysis demonstrated that the percentage of granulosa cells in S phase peaked 24-30 h post-PMSG and declined to control levels 48 h after PMSG administration. Expression of both Cyclin D2 and Cdk 4 was highest 12 h post-PMSG and decreased to control levels by 48 h. In addition, Cdk 2 protein increased transiently 12-24 h after PMSG. Cyclin E expression increased significantly by 12 h but remained elevated through 48 h, and multiple isoforms of Cyclin E were observed with increased proliferation. Both Cdk 4 and Cdk 2 activity parallel protein expression, although, changes in Cdk 2 were more marked. Levels of mRNA for the cell cycle inhibitors p21CIP1 and p27KIP1 increased significantly by 48 h post-PMSG. These results demonstrate that PMSG-stimulated movement of granulosa cells across the G1/S boundary during follicle growth is transient. In addition, the control of granulosa cell proliferation may reside through the regulation of both Cdk 2 and Cdk 4.

Original languageEnglish (US)
Pages (from-to)6-15
Number of pages10
JournalMolecular and Cellular Endocrinology
Volume264
Issue number1-2
DOIs
StatePublished - Jan 29 2007

Fingerprint

Cyclins
Cyclin-Dependent Kinases
Granulosa Cells
Cell proliferation
G1 Phase
S Phase
Cyclin E
Level control
Growth
Cell Proliferation
Cyclin D2
Gonadotropins
Rats
Protein Isoforms
Proteins
Cells
Messenger RNA
Cell Differentiation
Cell Cycle
Antral

Keywords

  • Cyclin
  • Follicle
  • Granulosa cell
  • Proliferation

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Granulosa cell expression of G1/S phase cyclins and cyclin-dependent kinases in PMSG-induced follicle growth. / Cannon, Jennifer D; Cherian-Shaw, Mary; Lovekamp-Swan, Tara; Chaffin, Charles L.

In: Molecular and Cellular Endocrinology, Vol. 264, No. 1-2, 29.01.2007, p. 6-15.

Research output: Contribution to journalArticle

Cannon, Jennifer D ; Cherian-Shaw, Mary ; Lovekamp-Swan, Tara ; Chaffin, Charles L. / Granulosa cell expression of G1/S phase cyclins and cyclin-dependent kinases in PMSG-induced follicle growth. In: Molecular and Cellular Endocrinology. 2007 ; Vol. 264, No. 1-2. pp. 6-15.
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