Granzyme-b mediated cell death in the spinal cord-injured rat model

Ganta Vijay Chaitanya, Mayuri Kolli, Phanithi Prakash Babu

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Spinal cord injury initiates a complex series of inflammatory and immune responses including the influx of monocytes, macrophages, T-cells, NK cells and so on, into the injured area. In the present study, we found a significant increase in the levels of granzyme-b (gra-b) from the first day after the transection until the third day, with decrease in intensity thereafter. The chemokine IP-10/CXCL10 was also found to be elevated along with gra-b correlating with the infiltration of CD-8+ cytotoxic T lymphocytes (CTLs) into the injured spinal cord. We observed an increase in the levels of the 64 kDa poly ADP ribose polymerase fragment, known to be a signature fragment produced by gra-b. Localization of gra-b in TUNEL positive neurons indicates that gra-b might play a crucial role in neuronal death and contributes to the pathophysiology of spinal cord injury.

Original languageEnglish (US)
Pages (from-to)270-279
Number of pages10
JournalNeuropathology
Volume29
Issue number3
DOIs
StatePublished - Jun 2009
Externally publishedYes

Keywords

  • Cytotoxic T lymphocytes
  • Granzyme-b
  • IP-10/CXCL10
  • PARP
  • Spinal cord injury

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Clinical Neurology

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