Greater fractalkine expression in mesenteric arteries of female spontaneously hypertensive rats compared with males

Jennifer C. Sullivan, Jennifer L. Pardieck, Derek Doran, Yan Zhang, Jin Xiong She, Jennifer S. Pollock

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A mircoarray analysis was performed to identify novel inflammatory genes that are differentially expressed in the mesenteric arteries of male and female spontaneously hypertensive rats (SHRs). Fractalkine was found to be the inflammatory gene with the greatest differential expression in mesenteric arteries, with the expression being greater in female SHRs compared with males. Greater inflammatory mediators in female SHRs were verified by measuring urinary monocyte chemoattractant protein-1, transforming growth factor-β, and tumor necrosis factor-α (TNF-α) excretion, all of which were greater in female SHRs compared with males. Real-time PCR, Western blot analysis, and ELISA verified greater soluble fractalkine in mesenteric arteries of female SHRs. Consistent with increased fractalkine expression, TNF-α-converting enzyme and TNF-α levels in mesenteric arteries were also greater in female SHRs. We next tested the hypothesis that mesenteric arteries from female SHRs will have greater fractalkine-induced dysfunction. Acetylcholine, sodium nitroprusside, phenylephrine, and KCl concentration-response curves were performed in third-order mesenteric arteries from male and female SHRs pretreated with either vehicle or fractalkine (1 μg/ml). Fractalkine decreased sensitivity to 1) acetylcholine in arteries from male SHRs, 2) phenylephrine in arteries from both sexes, and 3) KCl in arteries from female SHRs. In conclusion, urinary and vascular markers of inflammation are greater in female SHRs compared with males, although blood pressure and cardiovascular risk are less in females.

Original languageEnglish (US)
Pages (from-to)H1080-H1088
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume296
Issue number4
DOIs
StatePublished - Apr 1 2009

Fingerprint

Chemokine CX3CL1
Mesenteric Arteries
Inbred SHR Rats
Arteries
Tumor Necrosis Factor-alpha
Phenylephrine
Acetylcholine
Chemokine CCL2
Transforming Growth Factors
Nitroprusside
Genes
Blood Vessels
Real-Time Polymerase Chain Reaction

Keywords

  • Hypertension
  • Inflammation
  • Microarray
  • Sex difference
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Greater fractalkine expression in mesenteric arteries of female spontaneously hypertensive rats compared with males. / Sullivan, Jennifer C.; Pardieck, Jennifer L.; Doran, Derek; Zhang, Yan; She, Jin Xiong; Pollock, Jennifer S.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 296, No. 4, 01.04.2009, p. H1080-H1088.

Research output: Contribution to journalArticle

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