Abstract
Background/Aims: To investigate the inhibitory effect of green tea extract, epigallocatechin gallate (EGCG), on wild-type human leiomyoma (WT-HuLM) cells and its potential action via catechol-o-methyltransferase (COMT) activity. Methods: Cell proliferation of WT-HuLM and COMT gene-silenced HuLM (COMT-shRNA-HuLM) cells treated with 0 or 100 μM EGCG for 7 days was measured using the MTT method. Total RNA and protein were extracted from cells treated with 0 or 100 μM of EGCG for 48 h. Gene expression profiling was performed using Human Signal Transduction PathwayFinder. Proliferation cell nuclear antigen (PCNA), cyclin-dependent kinase 4 (Cdk4) and COMT protein levels were detected by Western blot analyses. COMT enzyme activity was evaluated by HPLC. Results: EGCG-treated WT-HuLM cells showed significantly decreased COMT expression (p < 0.001) and enzyme activity (p < 0.05) compared to untreated WT-HuLM cells, while COMT-shRNA-HuLM cells showed no significant change. At 100 μM of EGCG, survival of WT-HuLM cells was significantly lower (p < 0.05) compared to COMT-shRNA-HuLM cells. EGCG treatment modulated multiple signaling pathways in WT-HuLM compared to untreated control, while changes were minimal or reversed in COMT-shRNA-HuLM cells. EGCG significantly decreased PCNA, Cdk4 and soluble COMT protein levels (p < 0.001) in WT-HuLM, but not in COMT-shRNA-HuLM cells. Conclusions: The antiproliferative and gene-modulating effects of EGCG on HuLM cells are mediated, at least partially, via its effect on COMT expression and enzyme activity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 109-118 |
| Number of pages | 10 |
| Journal | Gynecologic and Obstetric Investigation |
| Volume | 78 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 1 2014 |
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Keywords
- Catechol-o-methyltransferase
- Epigallocatechin gallate
- Green tea
- Leiomyoma
- Proliferation inhibition
- Uterine fibroids
ASJC Scopus subject areas
- Reproductive Medicine
- Obstetrics and Gynecology
Cite this
Green tea extract inhibition of human leiomyoma cell proliferation is mediated via catechol-o-methyltransferase. / Zhang, Dong; Rajaratnam, Veera; Al-Hendy, Omar; Halder, Sunil Krishna; Al-Hendy, Ayman.
In: Gynecologic and Obstetric Investigation, Vol. 78, No. 2, 01.01.2014, p. 109-118.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Green tea extract inhibition of human leiomyoma cell proliferation is mediated via catechol-o-methyltransferase
AU - Zhang, Dong
AU - Rajaratnam, Veera
AU - Al-Hendy, Omar
AU - Halder, Sunil Krishna
AU - Al-Hendy, Ayman
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Background/Aims: To investigate the inhibitory effect of green tea extract, epigallocatechin gallate (EGCG), on wild-type human leiomyoma (WT-HuLM) cells and its potential action via catechol-o-methyltransferase (COMT) activity. Methods: Cell proliferation of WT-HuLM and COMT gene-silenced HuLM (COMT-shRNA-HuLM) cells treated with 0 or 100 μM EGCG for 7 days was measured using the MTT method. Total RNA and protein were extracted from cells treated with 0 or 100 μM of EGCG for 48 h. Gene expression profiling was performed using Human Signal Transduction PathwayFinder. Proliferation cell nuclear antigen (PCNA), cyclin-dependent kinase 4 (Cdk4) and COMT protein levels were detected by Western blot analyses. COMT enzyme activity was evaluated by HPLC. Results: EGCG-treated WT-HuLM cells showed significantly decreased COMT expression (p < 0.001) and enzyme activity (p < 0.05) compared to untreated WT-HuLM cells, while COMT-shRNA-HuLM cells showed no significant change. At 100 μM of EGCG, survival of WT-HuLM cells was significantly lower (p < 0.05) compared to COMT-shRNA-HuLM cells. EGCG treatment modulated multiple signaling pathways in WT-HuLM compared to untreated control, while changes were minimal or reversed in COMT-shRNA-HuLM cells. EGCG significantly decreased PCNA, Cdk4 and soluble COMT protein levels (p < 0.001) in WT-HuLM, but not in COMT-shRNA-HuLM cells. Conclusions: The antiproliferative and gene-modulating effects of EGCG on HuLM cells are mediated, at least partially, via its effect on COMT expression and enzyme activity.
AB - Background/Aims: To investigate the inhibitory effect of green tea extract, epigallocatechin gallate (EGCG), on wild-type human leiomyoma (WT-HuLM) cells and its potential action via catechol-o-methyltransferase (COMT) activity. Methods: Cell proliferation of WT-HuLM and COMT gene-silenced HuLM (COMT-shRNA-HuLM) cells treated with 0 or 100 μM EGCG for 7 days was measured using the MTT method. Total RNA and protein were extracted from cells treated with 0 or 100 μM of EGCG for 48 h. Gene expression profiling was performed using Human Signal Transduction PathwayFinder. Proliferation cell nuclear antigen (PCNA), cyclin-dependent kinase 4 (Cdk4) and COMT protein levels were detected by Western blot analyses. COMT enzyme activity was evaluated by HPLC. Results: EGCG-treated WT-HuLM cells showed significantly decreased COMT expression (p < 0.001) and enzyme activity (p < 0.05) compared to untreated WT-HuLM cells, while COMT-shRNA-HuLM cells showed no significant change. At 100 μM of EGCG, survival of WT-HuLM cells was significantly lower (p < 0.05) compared to COMT-shRNA-HuLM cells. EGCG treatment modulated multiple signaling pathways in WT-HuLM compared to untreated control, while changes were minimal or reversed in COMT-shRNA-HuLM cells. EGCG significantly decreased PCNA, Cdk4 and soluble COMT protein levels (p < 0.001) in WT-HuLM, but not in COMT-shRNA-HuLM cells. Conclusions: The antiproliferative and gene-modulating effects of EGCG on HuLM cells are mediated, at least partially, via its effect on COMT expression and enzyme activity.
KW - Catechol-o-methyltransferase
KW - Epigallocatechin gallate
KW - Green tea
KW - Leiomyoma
KW - Proliferation inhibition
KW - Uterine fibroids
UR - http://www.scopus.com/inward/record.url?scp=84908356609&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84908356609&partnerID=8YFLogxK
U2 - 10.1159/000363410
DO - 10.1159/000363410
M3 - Article
C2 - 24942317
AN - SCOPUS:84908356609
VL - 78
SP - 109
EP - 118
JO - Gynecologic and Obstetric Investigation
JF - Gynecologic and Obstetric Investigation
SN - 0378-7346
IS - 2
ER -