Green tea polyphenol induces caspase 14 in epidermal keratinocytes via MAPK pathways and reduces psoriasiform lesions in the flaky skin mouse model

Stephen Hsu, Douglas Dickinson, James Borke, Douglas S. Walsh, Joseph Wood, Haiyan Qin, Julia Winger, Henna Pearl, George Schuster, Wendy B Bollag

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Psoriasiform lesions are characterized by hyperproliferation and aberrant differentiation of epidermal keratinocytes, accompanied by inflammation, leading to a disrupted skin barrier with an abnormal stratum corneum. The expression and proteolytic processing of caspase 14, a member of the caspase family which is associated with epithelial cell differentiation, planned cell death, and barrier formation, is altered in psoriatic epidermis. We recently reported that human psoriatic tissues lack normal expression of caspase 14 [ J Dermatol Sci 37 (2005) 61], and caspase 14 is induced by EGCG, a green tea polyphenol (GTP), in exponentially growing normal human epidermal keratinocytes (NHEK) [ J Pharmacol Exp Ther 315 (2005) 805]. This suggests that GTPs may have beneficial effects on psoriasiform lesions. The current study aimed to determine whether MAPK pathways are required for GTP-induced caspase 14 expression in NHEK and if GTPs can modulate the expression of pathological markers in the psoriasiform lesions that develop in the flaky skin mouse. The results indicate that the p38 and JNK MAPK pathways are required for EGCG-induced expression of caspase 14 in NHEK. Importantly, topical application of 0.5% GTPs significantly reduced the symptoms of epidermal pathology in the flaky skin mice, associated with efficient caspase 14 processing and reduction in proliferating cell nuclear antigen levels. This suggests that GTP-activated pathways may be potential targets for novel therapeutic approaches to the treatment of some psoriasiform skin disorders.

Original languageEnglish (US)
Pages (from-to)678-684
Number of pages7
JournalExperimental Dermatology
Volume16
Issue number8
DOIs
StatePublished - Aug 1 2007

Fingerprint

Caspase 14
Polyphenols
Tea
Keratinocytes
Skin
Guanosine Triphosphate
MAP Kinase Signaling System
Proliferating Cell Nuclear Antigen
p38 Mitogen-Activated Protein Kinases
Pathology
Cell death
Caspases
Processing
Epidermis
Cornea
Cell Differentiation
Cell Death
Epithelial Cells
Tissue
Inflammation

Keywords

  • Caspase 14
  • EGCG
  • Epidermal
  • Green tea
  • Keratinocyte polyphenols
  • Psoriasiform lesions

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

Green tea polyphenol induces caspase 14 in epidermal keratinocytes via MAPK pathways and reduces psoriasiform lesions in the flaky skin mouse model. / Hsu, Stephen; Dickinson, Douglas; Borke, James; Walsh, Douglas S.; Wood, Joseph; Qin, Haiyan; Winger, Julia; Pearl, Henna; Schuster, George; Bollag, Wendy B.

In: Experimental Dermatology, Vol. 16, No. 8, 01.08.2007, p. 678-684.

Research output: Contribution to journalArticle

Hsu, Stephen ; Dickinson, Douglas ; Borke, James ; Walsh, Douglas S. ; Wood, Joseph ; Qin, Haiyan ; Winger, Julia ; Pearl, Henna ; Schuster, George ; Bollag, Wendy B. / Green tea polyphenol induces caspase 14 in epidermal keratinocytes via MAPK pathways and reduces psoriasiform lesions in the flaky skin mouse model. In: Experimental Dermatology. 2007 ; Vol. 16, No. 8. pp. 678-684.
@article{8b60229ff0f64cd08fbf51303687b52f,
title = "Green tea polyphenol induces caspase 14 in epidermal keratinocytes via MAPK pathways and reduces psoriasiform lesions in the flaky skin mouse model",
abstract = "Psoriasiform lesions are characterized by hyperproliferation and aberrant differentiation of epidermal keratinocytes, accompanied by inflammation, leading to a disrupted skin barrier with an abnormal stratum corneum. The expression and proteolytic processing of caspase 14, a member of the caspase family which is associated with epithelial cell differentiation, planned cell death, and barrier formation, is altered in psoriatic epidermis. We recently reported that human psoriatic tissues lack normal expression of caspase 14 [ J Dermatol Sci 37 (2005) 61], and caspase 14 is induced by EGCG, a green tea polyphenol (GTP), in exponentially growing normal human epidermal keratinocytes (NHEK) [ J Pharmacol Exp Ther 315 (2005) 805]. This suggests that GTPs may have beneficial effects on psoriasiform lesions. The current study aimed to determine whether MAPK pathways are required for GTP-induced caspase 14 expression in NHEK and if GTPs can modulate the expression of pathological markers in the psoriasiform lesions that develop in the flaky skin mouse. The results indicate that the p38 and JNK MAPK pathways are required for EGCG-induced expression of caspase 14 in NHEK. Importantly, topical application of 0.5{\%} GTPs significantly reduced the symptoms of epidermal pathology in the flaky skin mice, associated with efficient caspase 14 processing and reduction in proliferating cell nuclear antigen levels. This suggests that GTP-activated pathways may be potential targets for novel therapeutic approaches to the treatment of some psoriasiform skin disorders.",
keywords = "Caspase 14, EGCG, Epidermal, Green tea, Keratinocyte polyphenols, Psoriasiform lesions",
author = "Stephen Hsu and Douglas Dickinson and James Borke and Walsh, {Douglas S.} and Joseph Wood and Haiyan Qin and Julia Winger and Henna Pearl and George Schuster and Bollag, {Wendy B}",
year = "2007",
month = "8",
day = "1",
doi = "10.1111/j.1600-0625.2007.00585.x",
language = "English (US)",
volume = "16",
pages = "678--684",
journal = "Experimental Dermatology",
issn = "0906-6705",
publisher = "Wiley-Blackwell",
number = "8",

}

TY - JOUR

T1 - Green tea polyphenol induces caspase 14 in epidermal keratinocytes via MAPK pathways and reduces psoriasiform lesions in the flaky skin mouse model

AU - Hsu, Stephen

AU - Dickinson, Douglas

AU - Borke, James

AU - Walsh, Douglas S.

AU - Wood, Joseph

AU - Qin, Haiyan

AU - Winger, Julia

AU - Pearl, Henna

AU - Schuster, George

AU - Bollag, Wendy B

PY - 2007/8/1

Y1 - 2007/8/1

N2 - Psoriasiform lesions are characterized by hyperproliferation and aberrant differentiation of epidermal keratinocytes, accompanied by inflammation, leading to a disrupted skin barrier with an abnormal stratum corneum. The expression and proteolytic processing of caspase 14, a member of the caspase family which is associated with epithelial cell differentiation, planned cell death, and barrier formation, is altered in psoriatic epidermis. We recently reported that human psoriatic tissues lack normal expression of caspase 14 [ J Dermatol Sci 37 (2005) 61], and caspase 14 is induced by EGCG, a green tea polyphenol (GTP), in exponentially growing normal human epidermal keratinocytes (NHEK) [ J Pharmacol Exp Ther 315 (2005) 805]. This suggests that GTPs may have beneficial effects on psoriasiform lesions. The current study aimed to determine whether MAPK pathways are required for GTP-induced caspase 14 expression in NHEK and if GTPs can modulate the expression of pathological markers in the psoriasiform lesions that develop in the flaky skin mouse. The results indicate that the p38 and JNK MAPK pathways are required for EGCG-induced expression of caspase 14 in NHEK. Importantly, topical application of 0.5% GTPs significantly reduced the symptoms of epidermal pathology in the flaky skin mice, associated with efficient caspase 14 processing and reduction in proliferating cell nuclear antigen levels. This suggests that GTP-activated pathways may be potential targets for novel therapeutic approaches to the treatment of some psoriasiform skin disorders.

AB - Psoriasiform lesions are characterized by hyperproliferation and aberrant differentiation of epidermal keratinocytes, accompanied by inflammation, leading to a disrupted skin barrier with an abnormal stratum corneum. The expression and proteolytic processing of caspase 14, a member of the caspase family which is associated with epithelial cell differentiation, planned cell death, and barrier formation, is altered in psoriatic epidermis. We recently reported that human psoriatic tissues lack normal expression of caspase 14 [ J Dermatol Sci 37 (2005) 61], and caspase 14 is induced by EGCG, a green tea polyphenol (GTP), in exponentially growing normal human epidermal keratinocytes (NHEK) [ J Pharmacol Exp Ther 315 (2005) 805]. This suggests that GTPs may have beneficial effects on psoriasiform lesions. The current study aimed to determine whether MAPK pathways are required for GTP-induced caspase 14 expression in NHEK and if GTPs can modulate the expression of pathological markers in the psoriasiform lesions that develop in the flaky skin mouse. The results indicate that the p38 and JNK MAPK pathways are required for EGCG-induced expression of caspase 14 in NHEK. Importantly, topical application of 0.5% GTPs significantly reduced the symptoms of epidermal pathology in the flaky skin mice, associated with efficient caspase 14 processing and reduction in proliferating cell nuclear antigen levels. This suggests that GTP-activated pathways may be potential targets for novel therapeutic approaches to the treatment of some psoriasiform skin disorders.

KW - Caspase 14

KW - EGCG

KW - Epidermal

KW - Green tea

KW - Keratinocyte polyphenols

KW - Psoriasiform lesions

UR - http://www.scopus.com/inward/record.url?scp=34447501969&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34447501969&partnerID=8YFLogxK

U2 - 10.1111/j.1600-0625.2007.00585.x

DO - 10.1111/j.1600-0625.2007.00585.x

M3 - Article

VL - 16

SP - 678

EP - 684

JO - Experimental Dermatology

JF - Experimental Dermatology

SN - 0906-6705

IS - 8

ER -