Green tea polyphenol targets the mitochondria in tumor cells inducing caspase 3-dependent apoptosis

Stephen Hsu, Jill Lewis, Baldev Singh, Patricia V Schoenlein, Tokio Osaki, Mohammad Athar, Alan G. Porter, George Schuster

Research output: Contribution to journalArticle

71 Scopus citations

Abstract

Induction of apoptosis by green tea polyphenols has been observed in various tumor cell systems, but whether green tea polyphenol-induced apoptosis requires caspase 3 for execution has not been confirmed. We previously reported that green tea polyphenol-induced apoptosis involved Apaf-1 accumulation and caspase 3 activation in the cytosol. In the current study, tumor cells either with deleted caspase 3 gene or expressing wild-type caspase 3 were treated with increasing concentrations of green tea polyphenol(s), followed by morphological analysis and caspase 3 activity assay. The caspase 3 null parental cell line was further examined in comparison with a well-characterized, caspase 3 wild type oral carcinoma cell line by MTT assay and BrdU incorporation assay. The results demonstrated that, while the mitochondrial function gradually declined to insignificant levels, caspase 3 null cells did not undergo apoptosis, which suggested that green tea polyphenol-induced apoptosis is a mitochondria-targeted, caspase 3-executed mechanism.

Original languageEnglish (US)
Pages (from-to)1533-1539
Number of pages7
JournalAnticancer Research
Volume23
Issue number2 B
StatePublished - Mar 1 2003

Keywords

  • Apoptosis
  • Breast cancer
  • EGCG
  • Oral cancer
  • Polyphenols
  • Tea

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Hsu, S., Lewis, J., Singh, B., Schoenlein, P. V., Osaki, T., Athar, M., Porter, A. G., & Schuster, G. (2003). Green tea polyphenol targets the mitochondria in tumor cells inducing caspase 3-dependent apoptosis. Anticancer Research, 23(2 B), 1533-1539.