Green tea polyphenol targets the mitochondria in tumor cells inducing caspase 3-dependent apoptosis

Stephen Hsu, Jill Lewis, Baldev Singh, Patricia V Schoenlein, Tokio Osaki, Mohammad Athar, Alan G. Porter, George Schuster

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Induction of apoptosis by green tea polyphenols has been observed in various tumor cell systems, but whether green tea polyphenol-induced apoptosis requires caspase 3 for execution has not been confirmed. We previously reported that green tea polyphenol-induced apoptosis involved Apaf-1 accumulation and caspase 3 activation in the cytosol. In the current study, tumor cells either with deleted caspase 3 gene or expressing wild-type caspase 3 were treated with increasing concentrations of green tea polyphenol(s), followed by morphological analysis and caspase 3 activity assay. The caspase 3 null parental cell line was further examined in comparison with a well-characterized, caspase 3 wild type oral carcinoma cell line by MTT assay and BrdU incorporation assay. The results demonstrated that, while the mitochondrial function gradually declined to insignificant levels, caspase 3 null cells did not undergo apoptosis, which suggested that green tea polyphenol-induced apoptosis is a mitochondria-targeted, caspase 3-executed mechanism.

Original languageEnglish (US)
Pages (from-to)1533-1539
Number of pages7
JournalAnticancer Research
Volume23
Issue number2 B
StatePublished - Mar 1 2003

Fingerprint

Polyphenols
Tea
Caspase 3
Mitochondria
Apoptosis
Neoplasms
Null Lymphocytes
Cell Line
Bromodeoxyuridine
Cytosol
Carcinoma

Keywords

  • Apoptosis
  • Breast cancer
  • EGCG
  • Oral cancer
  • Polyphenols
  • Tea

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Green tea polyphenol targets the mitochondria in tumor cells inducing caspase 3-dependent apoptosis. / Hsu, Stephen; Lewis, Jill; Singh, Baldev; Schoenlein, Patricia V; Osaki, Tokio; Athar, Mohammad; Porter, Alan G.; Schuster, George.

In: Anticancer Research, Vol. 23, No. 2 B, 01.03.2003, p. 1533-1539.

Research output: Contribution to journalArticle

Hsu, S, Lewis, J, Singh, B, Schoenlein, PV, Osaki, T, Athar, M, Porter, AG & Schuster, G 2003, 'Green tea polyphenol targets the mitochondria in tumor cells inducing caspase 3-dependent apoptosis', Anticancer Research, vol. 23, no. 2 B, pp. 1533-1539.
Hsu, Stephen ; Lewis, Jill ; Singh, Baldev ; Schoenlein, Patricia V ; Osaki, Tokio ; Athar, Mohammad ; Porter, Alan G. ; Schuster, George. / Green tea polyphenol targets the mitochondria in tumor cells inducing caspase 3-dependent apoptosis. In: Anticancer Research. 2003 ; Vol. 23, No. 2 B. pp. 1533-1539.
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