TY - JOUR
T1 - Green tea polyphenols induce differentiation and proliferation in epidermal keratinocytes
AU - Hsu, Stephen
AU - Bollag, Wendy B.
AU - Lewis, Jill
AU - Huang, Qin
AU - Singh, Baldev
AU - Sharawy, Mohamed
AU - Yamamoto, Tetsuya
AU - Schuster, George
PY - 2003/7/1
Y1 - 2003/7/1
N2 - The most abundant green tea polyphenol, epigallocatechin-3- gallate (EGCG), was found to induce differential effects between tumor cells and normal cells. Nevertheless, how normal epithelial cells respond to the polyphenol at concentrations for which tumor cells undergo apoptosis is undefined. The current study tested exponentially growing and aged primary human epidermal keratinocytes in response to EGCG or a mixture of the four major green tea polyphenols. EGCG elicited cell differentiation with associated induction of p57/KIP2 within 24 h in growing keratinocytes, measured by the expression of keratin 1, filaggrin, and transglutaminase activity. Aged keratinocytes, which exhibited low basal cellular activities after culturing in growth medium for up to 25 days, renewed DNA synthesis and activated succinate dehydrogenase up to 37-fold upon exposure to either EGCG or the polyphenols. These results suggest that tea polyphenols may be used for treatment of wounds or certain skin conditions characterized by altered cellular activities or metabolism.
AB - The most abundant green tea polyphenol, epigallocatechin-3- gallate (EGCG), was found to induce differential effects between tumor cells and normal cells. Nevertheless, how normal epithelial cells respond to the polyphenol at concentrations for which tumor cells undergo apoptosis is undefined. The current study tested exponentially growing and aged primary human epidermal keratinocytes in response to EGCG or a mixture of the four major green tea polyphenols. EGCG elicited cell differentiation with associated induction of p57/KIP2 within 24 h in growing keratinocytes, measured by the expression of keratin 1, filaggrin, and transglutaminase activity. Aged keratinocytes, which exhibited low basal cellular activities after culturing in growth medium for up to 25 days, renewed DNA synthesis and activated succinate dehydrogenase up to 37-fold upon exposure to either EGCG or the polyphenols. These results suggest that tea polyphenols may be used for treatment of wounds or certain skin conditions characterized by altered cellular activities or metabolism.
UR - http://www.scopus.com/inward/record.url?scp=0037937302&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037937302&partnerID=8YFLogxK
U2 - 10.1124/jpet.103.049734
DO - 10.1124/jpet.103.049734
M3 - Article
C2 - 12663686
AN - SCOPUS:0037937302
SN - 0022-3565
VL - 306
SP - 29
EP - 34
JO - Journal of Pharmacology and Experimental Therapeutics
JF - Journal of Pharmacology and Experimental Therapeutics
IS - 1
ER -