GRO family chemokines are specialized for monocyte arrest from flow

David F. Smith, Elena Galkina, Klaus Ley, Yuqing Huo

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Chemokines participate in various processes of monocyte recruitment including monocyte arrest and migration. Our group and others have demonstrated that growth-related oncogene (GRO)-α (CXCL1) can support monocyte arrest in models of inflammation. Here we employed a parallel plate-flow chamber and Transwell reconstitution assay to test whether GRO family chemokines were sufficient for Mono Mac 6 (a human monocytic cell line) and isolated human monocyte recruitment. Our study shows that 1) GRO-α, -β (CXCL2), and -7 (CXCL3) all act as arrest chemokines for monocyte adhesion on vascular cell adhesion molecule (VCAM)-1 under flow in the presence of P-selectin; 2) CXCR2 is the functional receptor for GRO-family chemokines in monocyte arrest; however, CXCR2 is not an arrest chemokine receptor in general, since epithelial neutrophil-activating peptide ENA-78 failed to arrest monocytes; 3) GRO-α, -β, and -γ all fail to increase intracellular free Ca2+ or mediate monocyte chemotaxis; and 4) signaling through Gαi protein, phosphoinositide 3-kinase, and actin polymerization but not Ca 2+ mobilization or the mitogen-activated kinases p38 and MAPK/extracellular signal-related kinase are necessary for GRO-α-mediated Mono Mac 6 cell arrest under flow. We conclude that the GRO-family chemokines are specialized monocyte-arrest chemokines. Their role in monocyte recruitment in inflammation can be inhibited by blocking CXCR2 function or downstream signaling events.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume289
Issue number5 58-5
DOIs
StatePublished - Nov 1 2005
Externally publishedYes

Fingerprint

Oncogenes
Chemokines
Monocytes
Growth
Phosphotransferases
Inflammation
P-Selectin
1-Phosphatidylinositol 4-Kinase
Vascular Cell Adhesion Molecule-1
Chemokine Receptors
p38 Mitogen-Activated Protein Kinases
Chemotaxis
Mitogens
Polymerization
Actins
Neutrophils
Cell Line

Keywords

  • G protein
  • Growth-related oncogene
  • P-selectin
  • Phosphoinositide 3-kinase
  • Signaling
  • Vascular cell adhesion molecule-1

ASJC Scopus subject areas

  • Physiology

Cite this

GRO family chemokines are specialized for monocyte arrest from flow. / Smith, David F.; Galkina, Elena; Ley, Klaus; Huo, Yuqing.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 289, No. 5 58-5, 01.11.2005.

Research output: Contribution to journalArticle

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